The importance of particle size and DNA condensation salt for calcium phosphate nanoparticle transfection

Pedraza, Claudio E.; Bassett, D. C.; McKee, Marc D.; Nelea, Valentin; Gbureck, Uwe; Barralet, Jake E.

doi:10.1016/j.biomaterials.2008.04.043

Cited by 85 publications

(54 citation statements)

References 37 publications

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“…Moreover, taking advantage of the wide array of ligands, which can be linked to these polymeric nanoparticles, different strategies could be used to enhance transfection efficiency when needed. In this regard, phosphate calcium and virus capsid components (adenovirus or retrovirus) have demonstrated to be highly effective systems to increase gene delivery [32,33] and could easily be incorporated into our formulations, to boost transfection efficiency.…”

Section: Efficient Transfection Of Plasmid Dnabiotinylated Nps and Exmentioning

confidence: 99%

Biotinylated polyurethane-urea nanoparticles for targeted theranostics in human hepatocellular carcinoma

et al. 2015

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Over the past years, significant efforts have been devoted to explore novel drug delivery and detection strategies for simultaneous therapy and diagnostics. The development of biotinylated polyurethane-urea nanoparticles as theranostic nanocarriers for targeted drug and plasmid delivery, for fluorescence detection of human hepatocellular carcinoma cells, is described herein. These targeted nanoparticles are specifically designed to incorporate biotin into the polymeric matrix, since many tumor types overexpress receptors for biotin as a mechanism to boost uncontrolled cell growth. The obtained nanoparticles were spherical, exhibited an average diameter ranging 110-145 nm, and showed no cytotoxicity in healthy endothelial cells. Biotinylated nanoparticles are selectively incorporated into the perinuclear and nuclear area of the human hepatocellular carcinoma cell line, HepG2, in division, but not into growing, healthy, human endothelial cells. Indeed, the simultaneous incorporation of the anticancer drugs, phenoxodiol or sunitinib, together with plasmid DNA encoding green fluorescent protein, into these nanoparticles allows a targeted pharmacological antitumor effect and furthermore, selective transfection of a reporter gene, to detect these cancer cells. The combined targeted therapy and detection strategy described here could be exploited for liver cancer therapy and diagnostics, with a moderate safety profile, and may also be a potential tool for other types of cancer.

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Section: Efficient Transfection Of Plasmid Dnabiotinylated Nps and Exmentioning

confidence: 99%

Biotinylated polyurethane-urea nanoparticles for targeted theranostics in human hepatocellular carcinoma

et al. 2015

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“…As nucleic acids alone are unable to penetrate the cell wall, efficient carriers are required [728,729]. Nanodimensional calcium orthophosphates can be represented as a unique class of the non-viral vectors, which can serve as efficient and alternative DNA carriers for targeted delivery of genes [289,696,697,708,714,[730][731][732][733][734][735][736][737][738][739][740][741][742] and cells [567,[743][744][745][746][747][748][749]. The standard transfection method using calcium orthophosphates, first introduced by Graham and van der Eb in 1973 [748], is still used in biochemistry.…”

Section: Other Applicationsmentioning

confidence: 99%

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Dorozhkin¹

2012

AJBE

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Nano-sized particles and crystals play an important role in the formation of calcified tissues of various animals. For example, nano-sized and nanocrystalline calcium orthophosphates in the form of apatites of biological origin represent the basic inorganic building blocks of bones and teeth of mammals. Namely, according the recent developments in biomineralization, tens to hundreds nanodimensional crystals of a biological apatite are self-assembled into these complex structures. This process occurs under a strict control by bioorganic matrixes. Furthermore, both a greater viability and a better proliferation of various types of cells have been detected on smaller crystals of calcium orthophosphates. Thus, the nano-sized and nanocrystalline forms of calcium orthophosphates have a great potential to revolutionize the hard tissue-engineering field, starting from bone repair and augmentation to controlled drug delivery systems. This review reports on current state of the art and recent developments on the subject, starting from synthesis and characterization to biomedical and clinical applications. Furthermore, the review also discusses possible directions for future research and development.

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“…The DNA-coated calcium phosphate nanoparticles were 10-20 nm in diameter, showed good transfection efficiency, and could be stored for weeks without loss of their transfection activity. 7,8,13,14 Moreover, the efficiency of the multishell particles is significantly higher than that of simple DNA-coated calcium phosphate nanoparticles.…”

Section: Introductionmentioning

confidence: 95%

“…7,21 In the present study, the steric effect of the adsorption of protamine sulfate onto the surface of the DNA/calcium phosphate particles may play an important role in stabilizing their primary sizes and inhibiting their further growth. This effect is due to the possible existence of an optimum zeta potential for the adhesive interactions with cell membranes, which drives the nanoparticles to approach the cell surface and promote colloidal particle stability by electrostatic interaction.…”

mentioning

confidence: 99%

“…[4][5][6] Thus, the reproducibility of this method is poor compared with other transfection methods based on liposomes and polymers, and has to be optimized for every cell line and laboratory. 1,7 Furthermore, calcium phosphate particles grow with time, and consequently, its transfection efficiency is reduced. 1 The size of calcium phosphate particles greatly affects their transfection efficiency.…”

Section: Introductionmentioning

confidence: 99%

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An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery

Liu¹,

Wang²,

He³

et al. 2011

IJN

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Background: Smaller nanoparticles facilitate the delivery of DNA into cells through endocytosis and improve transfection efficiency. The aim of this study was to determine whether protamine sulfate-coated calcium phosphate (PS-CaP) could stabilize particle size and enhance transfection efficiency. Methods: pEGFP-C1 green fluorescence protein was employed as an indicator of transfection efficiency. Atomic force microscopy was used to evaluate the morphology and the size of the particles, and an MTT assay was introduced to detect cell viability and inhibition. The classical calcium phosphate method was used as the control. Results: Atomic force microscopy images showed that the PS-CaP were much smaller than classical calcium phosphate particles. In 293 FT, HEK 293, and NIH 3T3 cells, the transfection efficiency of PS-CaP was higher than for the classical calcium phosphate particles. The difference in efficiencies implies that the smaller nanoparticles may promote the delivery of DNA into cells through endocytosis and could improve transfection efficiency. In addition, PS-CaP could be used to transfect HEK 293 cells after one week of storage at 4°C with a lesser extent of efficiency loss compared with classical calcium phosphate, indicating that protamine sulfate may increase the stability of calcium phosphate nanoparticles. The cell viability inhibition assay indicated that both nanoparticles show similar low cell toxicity. Conclusion: PS-CaP can be used as a better nonviral transfection vector compared with classical calcium phosphate.

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The importance of particle size and DNA condensation salt for calcium phosphate nanoparticle transfection

Cited by 85 publications

References 37 publications

Biotinylated polyurethane-urea nanoparticles for targeted theranostics in human hepatocellular carcinoma

Biotinylated polyurethane-urea nanoparticles for targeted theranostics in human hepatocellular carcinoma

Nanodimensional and Nanocrystalline Calcium Orthophosphates

An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery

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