The importance of molecular analyses for understanding the genetic diversity of Histoplasma capsulatum: An overview

Vite-Garín, Tania; Estrada-Bárcenas, Daniel Alfonso; Cifuentes, Joaquín; Taylor, Mária Lucía

doi:10.1016/j.riam.2013.09.013

Cited by 16 publications

(11 citation statements)

References 30 publications

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“…There is, however, no consensus genotyping method for this fungus. Although outbreaks of histoplasmosis are regularly reported (324), the current genotyping studies are more focused on the genetic variation and environmental dispersion of H. capsulatum (325).…”

Section: Histoplasma Capsulatummentioning

confidence: 99%

Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?

et al. 2017

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Genotyping studies of medically important fungi have addressed elucidation of outbreaks, nosocomial transmissions, infection routes, and genotype-phenotype correlations, of which secondary resistance has been most intensively investigated. Two methods have emerged because of their high discriminatory power and reproducibility: multilocus sequence typing (MLST) and microsatellite length polymorphism (MLP) using short tandem repeat (STR) markers. MLST relies on single-nucleotide polymorphisms within the coding regions of housekeeping genes. STR polymorphisms are based on the number of repeats of short DNA fragments, mostly outside coding regions, and thus are expected to be more polymorphic and more rapidly evolving than MLST markers. There is no consensus on a universal typing system. Either one or both of these approaches are now available for spp., spp., spp., spp., ,, and endemic mycoses. The choice of the method and the number of loci to be tested depend on the clinical question being addressed. Next-generation sequencing is becoming the most appropriate method for fungi with no MLP or MLST typing available. Whatever the molecular tool used, collection of clinical data (e.g., time of hospitalization and sharing of similar rooms) is mandatory for investigating outbreaks and nosocomial transmission.

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Section: Histoplasma Capsulatummentioning

confidence: 99%

Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?

et al. 2017

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“…capsulatum sporadically, however they could play role in its dispersal [ 19 ]. In endemic areas of histoplasmosis throughout the Americas, bats are often infected with this fungus [ 20 , 21 ]. In addition to bats, the fungus has been detected in wild mammals such as non-human primates (e.g., baboons) [ 22 , 23 ], mustelids (e.g., badgers and northern sea otter) [ 24 – 26 ], procyonids (e.g., raccoons) [ 27 ], as well as in domesticated animals such as equines [ 28 , 29 ], felines [ 30 , 31 ] and canines [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

“…capsulatum sensu stricto . Techniques such as Restriction Fragment Length Polymorphisms (RFLP), DNA hybridization, Random Amplified Polymorphic DNA (RAPD) and ITS1/2 sequencing all revealed high genetic diversity in Histoplasma , with some indication of geographical association [ 21 ]. Population structure based on molecular genotyping suggested different patterns of genetic variation among H .…”

Section: Introductionmentioning

confidence: 99%

Worldwide Phylogenetic Distributions and Population Dynamics of the Genus Histoplasma

et al. 2016

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BackgroundHistoplasma capsulatum comprises a worldwide complex of saprobiotic fungi mainly found in nitrogen/phosphate (often bird guano) enriched soils. The microconidia of Histoplasma species may be inhaled by mammalian hosts, and is followed by a rapid conversion to yeast that can persist in host tissues causing histoplasmosis, a deep pulmonary/systemic mycosis. Histoplasma capsulatum sensu lato is a complex of at least eight clades geographically distributed as follows: Australia, Netherlands, Eurasia, North American classes 1 and 2 (NAm 1 and NAm 2), Latin American groups A and B (LAm A and LAm B) and Africa. With the exception of the Eurasian cluster, those clades are considered phylogenetic species.Methodology/Principal FindingsIncreased Histoplasma sampling (n = 234) resulted in the revision of the phylogenetic distribution and population structure using 1,563 aligned nucleotides from four protein-coding regions. The LAm B clade appears to be divided into at least two highly supported clades, which are geographically restricted to either Colombia/Argentina or Brazil respectively. Moreover, a complex population genetic structure was identified within LAm A clade supporting multiple monophylogenetic species, which could be driven by rapid host or environmental adaptation (~0.5 MYA). We found two divergent clades, which include Latin American isolates (newly named as LAm A1 and LAm A2), harboring a cryptic cluster in association with bats.Conclusions/SignificanceAt least six new phylogenetic species are proposed in the Histoplasma species complex supported by different phylogenetic and population genetics methods, comprising LAm A1, LAm A2, LAm B1, LAm B2, RJ and BAC-1 phylogenetic species. The genetic isolation of Histoplasma could be a result of differential dispersion potential of naturally infected bats and other mammals. In addition, the present study guides isolate selection for future population genomics and genome wide association studies in this important pathogen complex.

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“… División: Ascomycota Histoplasma -estado anamorfo  Especie: capsulata (estado teleomorfo), capsulatum, (estado anamorfo) (Peterson et al, 1998;Vite-Garín et al, 2014) Hc var. capsulatum es un hongo termodimórfico que a temperaturas inferiores a 35° C crece como un hongo micelial entre los 10 y 14 días.…”

Section: Clasificación Taxonómicaunclassified

Histoplasmosis en caninos y felinos: signos clínicos, métodos de diagnóstico y tratamiento

Cepeda¹,

Ruales²

2017

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La histoplasmosis es una enfermedad zoonótica, causada por el hongo dimórfico Histoplasma capsulatum, endémica en zonas tropicales, subtropicales y templadas de América y África. Suelos con abundante materia orgánica, excremento de aves y guano de murciélagos constituyen el nicho ecológico de este microorganismo. Esta micosis puede afectar a gran variedad de mamíferos. En esta revisión bibliográfica se aborda el tema de la histoplasmosis canina y felina, haciendo referencia, principalmente, a los métodos diagnósticos directos e indirectos empleados para su identificación. Además, se describen las manifestaciones clínicas características de cada fase de la enfermedad y su tratamiento y se presenta un algoritmo de diagnóstico.

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The importance of molecular analyses for understanding the genetic diversity of Histoplasma capsulatum: An overview

Cited by 16 publications

References 30 publications

Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?

Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?

Worldwide Phylogenetic Distributions and Population Dynamics of the Genus Histoplasma

Histoplasmosis en caninos y felinos: signos clínicos, métodos de diagnóstico y tratamiento

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