The Importance of miRNA Identification During Respiratory Viral Infections

Martínez-Espinoza, Iván; Baños-Lara, Ma. Del Rocío; Guerrero-Plata, Antonieta

doi:10.33696/immunology.3.101

Cited by 7 publications

(12 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cellular and viral miRNAs with roles in different viral infections are summarized in Supplementary Tables S2–S11 [ 224 , 225 , 226 , 228 , 229 , 230 , 231 , 232 , 233 , 234 , 235 , 236 , 237 , 238 , 239 , 240 , 241 , 242 , 243 , 244 , 245 ].…”

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

“…The most studied cellular miRNAs for which its expression is deregulated during HCV infection are miR122 and miR155 [ 242 , 243 ]. It is believed that HCV does not encode rv-miRNAs [ 248 ].…”

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

“…H5N1, a subtype of IAV, causes different expressions of different miRNAs in infected cells [ 243 , 244 ]. H5N1’s first-observed encoded v-miRNA was miRHA-3p, and it regulates the expression of the PCBP2 gene.…”

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

“…Coronaviruses are RNA viruses that cause severe acute respiratory syndrome, leading to high mortality [ 245 ]. Since 2020, due to the pandemic caused by COVID-19 infections, the interactions between cellular miRNAs and v-miRNAs have been meticulously studied [ 243 ]. To this date, 40 SARS-Cov-2-encoded miRNAs have been found, such as miR618, miR6501-5p, and miR144-3p.…”

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

“…All miRNAs were upregulated in COVID-19-positive patients. Their immunological implication in the course of the infection is not yet fully understood, but a few studies suggest that these miRNAs function through NFκB, JAK/STAT or TGFβ signaling pathways [ 243 ].…”

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

See 4 more Smart Citations

miRNA in Molecular Diagnostics

et al. 2022

View full text Add to dashboard Cite

MicroRNAs are a class of small non-coding RNA molecules that regulate gene expression on post-transcriptional level. Their biogenesis consists of a complex series of sequential processes, and they regulate expression of many genes involved in all cellular processes. Their function is essential for maintaining the homeostasis of a single cell; therefore, their aberrant expression contributes to development and progression of many diseases, especially malignant tumors and viral infections. Moreover, they can be associated with certain states of a specific disease, obtained in the least invasive manner for patients and analyzed with basic molecular methods used in clinical laboratories. Because of this, they have a promising potential to become very useful biomarkers and potential tools in personalized medicine approaches. In this review, miRNAs biogenesis, significance in cancer and infectious diseases, and current available test and methods for their detection are summarized.

show abstract

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

Section: Mirna In Viral Diseasesmentioning

confidence: 99%

See 3 more Smart Citations

miRNA in Molecular Diagnostics

et al. 2022

View full text Add to dashboard Cite

show abstract

Adenovirus 14p1 induced changes in miRNA expression increases lung immunopathogenesis

McIndoo

Wood

Kuffel

et al. 2022

Preprint

View full text Add to dashboard Cite

Adenovirus is a frequent cause of mild, usually self-limited infections in infants and young children. Severe infections occur in immunocompromised patients but are rarely observed in healthy, immunocompetent adults. However, there have been outbreaks around the world of infections with different adenoviral (Ad) serotypes that have resulted in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) in some of those infected. Ad14p1, the predominant circulating strain of Ad14 worldwide is one such serotype. The explanations for the severity of illness caused by Ad14p1 infection in immunocompetent patients is unknown. Previously, we have shown that A549 cells infected with Ad14 repress macrophage pro-inflammatory responses whereas cells infected with Ad14p1 fail to repress macrophages and, instead, can increase pro-inflammatory responses. Micro-RNAs (miRNA) are small noncoding RNAs that regulate gene expression at the posttranscriptional level. Adenoviral infection has been shown to modulate host miRNA expression, and we hypothesized that differences in miRNA expression between Ad14 and Ad14p1 infected cells might impact pathogenesis. A549 cells were infected with either Ad14 or Ad14p1 and total RNA samples were collected at 6, 12, 24, 36 and 48 post infection for miRNA sequencing. Cluster analysis revealed that there were 3 temporal changes in miRNA expression profiles following infection. Differential expression analysis showed 8-23 differentially expressed miRNA between Ad14 and Ad14p1 from 6 to 36hpi. However, at 48hpi there were 98 differentially expressed miRNAs in Ad14p1 infected cells compared to those infected by Ad14. Pathway enrichment analysis showed that the differentially expressed miRNA might explain the increased pathogenesis of Ad14p1caused by strain-related loss of modulation of cytokine expression. Overall, the data suggest a role for viral regulation of host miRNA expression in pathogenesis by regulating host inflammatory responses through the delivery of deregulated miRNAs by virally infected cell corpses to macrophages.

show abstract

Effect of Age, Sex, and COVID-19 Vaccination History on All-Cause Mortality: Unexpected Outcomes in a Complex Biological and Social System

Jones¹,

Ponomarenko²

2023

Preprint

View full text Add to dashboard Cite

All vaccines exhibit both specific and non-specific effects. The specific effects are measured by the efficacy against the target pathogen, while the non-specific effects can be detected by the change in all-cause mortality . All-cause mortality data (gender, age band, vaccination history, month of death) between January 2021 and May 2022 was compiled by the Office for National Statistics. COVID–19 vaccination gave good protection on many occasions but less so for younger ages. Each gender and age group shows its own unique vaccination benefit/disbenefit time profile. Individuals are free to make vaccination decisions. For example, women aged 18-39 show a cohort who do not progress beyond the first or second dose. The all-cause mortality outcomes for the Omicron variant showed a very poor response to vaccination with 70% of sex/age/vaccination stage/month combinations increasing all-cause mortality, probably due to unfavorable antigenic distance between the first-generation vaccines and this variant, and additional non-specific effects. The all-cause mortality outcomes of COVID–19 vaccination is far more nuanced than have been widely appreciated, and virus vector appear better than the mRNA vaccines in this specific respect. The latter are seemingly more likely to increase all-cause mortality especially in younger age groups. An extensive discussion/literature review is included to provide potential explanations for the observed unexpected vaccine effects.

show abstract

The Importance of miRNA Identification During Respiratory Viral Infections

Cited by 7 publications

References 52 publications

miRNA in Molecular Diagnostics

miRNA in Molecular Diagnostics

Adenovirus 14p1 induced changes in miRNA expression increases lung immunopathogenesis

Effect of Age, Sex, and COVID-19 Vaccination History on All-Cause Mortality: Unexpected Outcomes in a Complex Biological and Social System

Contact Info

Product

Resources

About