The importance of glutathione and phytochelatins on the selenite and arsenate detoxification in Arabidopsis thaliana

Aborode, Fatai Adigun; Raab, Andrea; Voigt, Matthias; Costa, Letícia M.; Krupp, Eva M.; Feldmann, Jörg

doi:10.1016/j.jes.2016.08.009

Cited by 44 publications

(25 citation statements)

References 54 publications

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“…Glutathione (GSH) and thioredoxin reductase (TrxR) were thought to play an important role in determining the redox state of cells , and GSH was also involved in selenate/selenite reduction . Thus, five genes gs , gst , gr , gpx , and trx B involving GSH metabolism, antioxidant, and redox regulation were selected to test if selenate affected the expression of these genes at the transcriptional level.…”

Section: Resultsmentioning

confidence: 99%

“…Toxic effects were initially thought to be due to its nonspecific incorporation into proteins by replacing sulphur in Cys and Met residues . At present, toxic effects are widely thought to result from its interaction with essential sulfhydryl‐containing enzymes and structural proteins, which led to oxidative stress at high concentrations . According to this theory, the oxidative stress of bacterial cells should be enhanced with the increase of selenate concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Both NADPH and GSH concentrations are thought to be related to the redox state of cells because TrxR and GR transfer reducing equivalents from NADPH to thioredoxin (Trx) and glutathione disulfide, respectively, and form Trx(SH) 2 and GSH acting as effective intracellular antioxidants . A balance between oxidant and antioxidant intracellular systems is vital for cell function, regulation, and adaptation to diverse growth conditions . In plants and mammals, TrxR in conjunction with Trx, as a ubiquitous oxidoreductase system, plays an important role in antioxidant and redox regulation , and this oxidoreductase system has been positioned at the core of cellular thiol redox control and antioxidant defense based on the properties of TrxR and the functions of Trx .…”

Section: Discussionmentioning

confidence: 99%

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Bacterial growth, morphology, and cell component changes in Herbaspirillum sp. WT00C exposed to high concentration of selenate

et al. 2020

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Selenium (Se) is a nonmetallic element of the chalcogens. It is primarily available in natural environments as selenate and selenite oxoanions. Although selenate/selenite reduction in many microbes is widely studied at low concentrations (<50 mM), the effects of high selenate stress on bacterial growth, morphology, and cell components have not yet been studied. In this study, the response of Herbaspirillum sp. WT00C to selenate stress at high concentration is investigated by microbiological and scanning electron microscopy (SEM) techniques as well as proteomic analysis. Bacterial growth was seriously inhibited under high selenate concentrations and its growth‐inhibitory phase was prolonged with the increase of selenate concentrations. More interestingly, this bacterium was able to recover its growth even if the selenate concentration was up to 400 mM. Its growth inhibition period shortened to 6 h when the bacterium growing in 200 mM selenate for 28 h was reinoculated to the Luria‐Bertani medium containing 200 mM selenate. The high concentration of selenate also induces marked changes in the cell dimension and surface roughness, as revealed by SEM, along with compositional changes in the cell wall shown by proteomic analysis. The bacterial growth inhibition results from the marked downregulation of the α‐subunit of DNA polymerase III and RNA helicase, whereas its growth recovery is related to its high antioxidative activities. More NADPH synthesis and the upregulation of thioredoxin reductase and GPx are beneficial for Herbaspirillum sp. WT00C to establish and maintain a balance between oxidant and antioxidant intracellular systems for defending selenate toxicity. This study is an important contribution to understanding why Herbaspirillum sp. WT00C survives in a high concentration of selenate and how the bacterial cells respond physiologically to selenate stress at high concentration.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Bacterial growth, morphology, and cell component changes in Herbaspirillum sp. WT00C exposed to high concentration of selenate

et al. 2020

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“…Generation of CRp has been studied in a wide variety of plants and microorganisms. It has been shown that CRp could be taken as a response to heavy metal contamination and it is understood that the best trigger of cysteine-rich peptides generation is Cd, followed by Pb, Hg, and Zn [2,3].…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of Copper Electrodes, Modified with Bimetallic Nanoparticles, to be Used as Sensors of Cysteine-Rich Peptides Synthesized by Tobacco Cells Exposed to Cytotoxic Levels of Cadmium

et al. 2019

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We report on two new electrochemical sensors which, coupled to differential pulse voltammetry, constitutes a useful tool for diagnosis of heavy metal pollution. The electrochemical sensors AgHgNf/Cu and the AgBiNf/Cu were obtained by deposition of bimetallic particles of AgHg or AgBi on copper electrodes covered with a Nafion (Nf) film, respectively. Micrographs of the electrode’s surface showed evenly scattered bimetallic particles, with an approximate diameter of 150 nm, embedded in the Nafion (Nf) film. In order to test the electrodes, the hydrogen evolution signal according to the Brdička reaction was measured for the determination of cysteine-rich peptides (CRp) produced by plants. To check the accuracy of the electrodes, real samples of Nicotiana tabacum cells exposed to cytotoxic levels of cadmium were tested. The AgHgNf/Cu electrode produced detection limits (DLs) of 0.088 µmol L−1 for Cysteine and 0.139µmol L−1 for Glutathione, while for the AgBiNf/Cu electrode DLs were 0.41 µmol L−1 for cysteine and 0.244 µmol L−1 for glutathione. Thus, the new electrodes could be a useful analytical electrochemical system very convenient for fieldwork. The electrodes were capable of direct, accurate, and sensitive detection of synthesized peptides, despite the complex matrix where the Nicotiana tabacum cells were grown.

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“…Whereas sulfur is heavily interfered with by polyatomic oxygen species, and bromine is heavily interfered with by argides, chlorine and fluorine are not efficiently ionized in an argon plasma because of their extremely high ionization potentials. Although sulfur has been monitored to support the determination of thioarsenicals [ 36 ] or cysteine-bound elements such as selenium [ 37 ], selective non-targeted analysis for sulfur has not been reported. However, with the help of different ICPMS technologies such as high-resolution ICPMS [ 29 ] or ICPMS–MS [ 38 ] or simply the use of ultrasonic nebulizers with desolvation [ 39 ], the detection limits can be lowered to a few micrograms of sulfur per litre per eluted species.…”

Section: Non-targeted Speciation Analysis (Status Quo and Outlook)mentioning

confidence: 99%

Importance of ICPMS for speciation analysis is changing: future trends for targeted and non-targeted element speciation analysis

2017

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This article is aimed at researchers interested in organic molecules which contain a heteroatom but who have never considered using inductively coupled plasma mass spectrometry (ICPMS) or who have used ICPMS for years and developed numerous methods for analysis of target elemental species. We try to illustrate (1) that ICPMS has been very useful for speciation analysis of metal(loid) target species and that there is now a trend to replace the costly detector with cheaper detection systems for routine target analysis, and (2) that ICPMS has been used and will be used even more in the future for non-targeted analysis of elements which are not normally associated with ICPMS analysis, such as non-metals such as sulfur, phosphorus, chlorine and fluorine. Graphical AbstractStarting with HPLC-ICPMS for non-targeted analysis of heteroatom containing molecules, once target molecule is identified alternative detectors can be used for routine measurements

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The importance of glutathione and phytochelatins on the selenite and arsenate detoxification in Arabidopsis thaliana

Cited by 44 publications

References 54 publications

Bacterial growth, morphology, and cell component changes in Herbaspirillum sp. WT00C exposed to high concentration of selenate

Bacterial growth, morphology, and cell component changes in Herbaspirillum sp. WT00C exposed to high concentration of selenate

Development and Evaluation of Copper Electrodes, Modified with Bimetallic Nanoparticles, to be Used as Sensors of Cysteine-Rich Peptides Synthesized by Tobacco Cells Exposed to Cytotoxic Levels of Cadmium

Importance of ICPMS for speciation analysis is changing: future trends for targeted and non-targeted element speciation analysis

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