The importance of ctokeratins in the early detection of oral squamous cell carcinoma

Abstract

Background: Oral cancer is usually diagnosed at advanced stages. The pattern of keratin expression in normal epithelia and the change in their expression in premalignant lesions and carcinomas have suggested the possibilities of improving diagnosis. The aim of this study is to determine the use of acidic cytokeratins (CKs) as biomarkers of histopathological progression in oral carcinogenesis. Materials and Methods: A total of 50 paraffin blocks of histological specimens diagnosed as hyperplastic epithelium, dysplastic epithelium, well-differentiated squamous cell carcinoma (SCC) and poorly-differentiated SCC (10 specimens each) were included in this study, in addition to 10 normal oral mucosal samples. All samples were stained immunohistochemically with CKs (10-ab1, 14, 16-ab1, 18-dc10 and 19-abs10) using Ventana Medical Systems (Arizona-USA). The expression of CKs antigen was evaluated as absent, mild, moderate and severe. Results: CK10-ab1 was found to be positive in the suprabasal layers of all specimens in normal and hyperplastic epithelium, while it was moderate in dysplastic epithelium and mild in well-differentiated SCC. CK10-ab1 was negative in all samples with poorly-differentiated SCC ( P < 0.005). CK14 was positive in all specimens of all groups whereas CK16-ab1 was negative in all specimens of all groups. The stain of CKs 18-dc10 and 19-abs10 was restricted to the basal cells only in normal, hyperplastic and dysplastic epithelium, while it was mild in well-differentiated and poorly-differentiated SCC ( P < 0.01). Conclusion: CK10-ab1 disappeared gradually with the progression of malignant changes of squamous cells whereas CKs 18-dc10 and 19-abs10 increased gradually at the same time. Such changes in the protein mapping of squamous cells need more investigation for a better understanding of oral SCC.

Integrated proteomics and phosphoproteomics revealed druggable kinases in neoadjuvant chemotherapy resistant tongue cancer

“…Our proteomic data revealed higher expression type II keratin, KRT1, and its heterodimer type I partner KRT10 along with other keratins such as KRT72, KRT71, and KRT84. Previous studies have linked altered KRT10 expression to the dysplastic progression of oral cancer ( Ali et al, 2018 ). In addition, expression of Keratin 1/10 was identified in premalignant oral lesions ( Kannan et al, 1994 ; Ali et al, 2018 ).…”

“…Previous studies have linked altered KRT10 expression to the dysplastic progression of oral cancer ( Ali et al, 2018 ). In addition, expression of Keratin 1/10 was identified in premalignant oral lesions ( Kannan et al, 1994 ; Ali et al, 2018 ). Interestingly, we also observed high and extensive phosphorylation of type I (KRT) and type II (KRT) keratins.…”

Consistent downregulation of the cleft lip/palate-associated genes IRF6 and GRHL3 in carcinomas

“…Overexpression of IRF6 and GRHL3 in SCC-68 partially normalized this differentiation defect and K10 was reinduced in an IRF6 and/or GRHL3-dependent way (Figure 4). K10, which is restricted to post-mitotic cells in the spinous layer of the skin, has been found gradually disappearing with SCC progression and strongly correlating with the differentiation status of SCCs (55)(56)(57)(58). Lack of K10 results in defects in skin barrier repair upon experimental barrier disruption, skin hydration, and acid sphingomyelinase activity (59).…”

Expression of cornulin in tongue squamous cell carcinoma

“…Cytokeratins (CKs) have been reported to be indicative of squamous cell differentiation [ 24 ]. Keratin 4 (CK4) is a marker of dysregulation of oral epithelial differentiation [ 25 ], CK10 expressed strongly in squamous cells [ 26 ], CK14 fell as the severity of the disease progressed from low- to high-grade dysplasia to SCC [ 27 ]. CK1/10 are markers for keratinised epithelium and CK4/13 can be used as markers of non-keratinised epithelium [ 28 ].…”