The importance of adequate reduction of peptone in the preparation of media for the pneumococcus and other organisms

Wright, Hedley D.

doi:10.1002/path.1700370208

Cited by 56 publications

(22 citation statements)

References 29 publications

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“…In the growth of pneumococci in fluid media it is common practice to use Wright's broth (Wright, 1933) or its equivalent, supplemented with serum and glucose. This medium has two disadvantages: ( a ) the serum is expensive and contaminates the microbes with antigenic foreign protein; ( b ) the incubation of the culture has to be closely watched since a t a certain stage of growth marked autolysis takes place.…”

Section: Introductionmentioning

confidence: 99%

The Culture of Streptococcus pneumoniue

Holt

1962

Journal of General Microbiology

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SUMMARYIn the fluid culture of Streptococcus pneumoniue in glucose + serum broth the pa.rt played by the serum appeared to be solely the regulation of pH value, and may be substituted by a suitable amount of sodium carbonate. The reason for the autolysis experienced in the fluid culture of pneumococci would appear to be due to hydrogen peroxide production.The addition of cysteine to a glucose + carbonate broth precluded the autolytic effect either by the cysteine giving an anaerobic condition of growth or by destroying HgO,.

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Section: Introductionmentioning

confidence: 99%

The Culture of Streptococcus pneumoniue

Holt

1962

Journal of General Microbiology

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“…The enumeration of different microorganisms was done using standard spread plate technique. The bacterial count was enumerated on nutrient agar (Wright, 1933) media plates, fungi enumerated on glucose yeast extract (GYE) media, diazotrophs enumerated on Burk's agar and PGPR's on King's B media. Microbial count was made 30 days after planting (DAP).…”

Section: Methodsmentioning

confidence: 99%

Influence of Mulching and Graded Fertility Levels on Microbial Population, Growth and Productivity of Potato (Solanum tuberosum L.)

Mukherjee¹

2017

Int.J.Curr.Microbiol.App.Sci

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“…A 24-hr. culture of Staphylococcus aureus (various strains were used) in Wright's broth (Wright, 1933) was centrifuged and the cells suspended in sterile 0-85 yo saline. Large clumps of cells were broken up by shaking with glass beads and the strength of the suspension determined by comparison with standard opacity tubes.…”

Section: Experimental Methodsmentioning

confidence: 99%

Factors Influencing the in vitro Production of Staphylococcal Coagulase

Davies

1951

Journal of General Microbiology

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SUMMARY: The amount of coagulase formed in vitro by coagulase-positive staphylococci depends on: the age of the bacterial cells, coagulase being produced only during the latter part of the lag-phase of growth; the period of incubation of the bacterial cells under ideal conditions, the optimum period depending upon the age of the cells when incubation starts; the initial concentration of bacteria. It also depends on the presence in the medium of a fermentable sugar and a factor found in brain-heart infusion and partially replaceable by peptone; the salt concentration in the medium; the initial pH value of the medium; the temperature of incubation: and upon substances present in rabbit serum and, to a lesser extent, horse serum, human serum and egg yolk, egg white and commercial nucleic acid not showing this effect. The activity of rabbit serum is destroyed by treatment with pepsin. Provided that the requisite conditions are met, cell-free coagulase which clots rabbit plasma within a few minutes, can be prepared in less than one hour.Since the demonstration that coagulase is an important factor in determining the pathogenicity of staphylococci, many papers on this substance have appeared (e.g. Christie

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The importance of adequate reduction of peptone in the preparation of media for the pneumococcus and other organisms

Cited by 56 publications

References 29 publications

The Culture of Streptococcus pneumoniue

The Culture of Streptococcus pneumoniue

Influence of Mulching and Graded Fertility Levels on Microbial Population, Growth and Productivity of Potato (Solanum tuberosum L.)

Factors Influencing the in vitro Production of Staphylococcal Coagulase

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