The imperative role of polymers in enzymatic cholesterol biosensors- an overview

Antony, Neethu; Unnikrishnan, Lakshmi; Mohanty, Smita; Nayak, Sanjay K.

doi:10.1080/25740881.2019.1576197

Cited by 5 publications

(2 citation statements)

References 196 publications

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“…The advantage is that the high concentration of enzyme under mild conditions can effectively prevent enzyme shedding. The disadvantage is the pH value and temperature of the polymer solution are strictly controlled ( Ji et al, 2017 ; Antony et al, 2019 ; Boselli et al, 2021 ; Coşkuner Filiz et al, 2021 ).…”

Section: Electrospun Nanofiber-based Enzymatic and Non-enzymatic Gluc...mentioning

confidence: 99%

“…Enzymatic glucose detection involves the oxidation reaction of glucose, reaction mediators, and enzymes. One of the most commonly used enzymes is glucose oxidase (GOx), which is used in glucose sensors as a catalyst, mainly due to the higher sensitivity and selectivity of GOx in response to glucose (Antony et al, 2019;Puttananjegowda et al, 2020a). Non-enzymatic glucose detection involves the oxidation reaction of glucose, which eliminates the need for reaction mediators and enzymes.…”

Section: Principles Of Glucose Sensorsmentioning

confidence: 99%

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Electrospun nanofiber-based glucose sensors for glucose detection

Zhang

Liu

et al. 2022

Front. Chem.

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Diabetes is a chronic, systemic metabolic disease that leads to multiple complications, even death. Meanwhile, the number of people with diabetes worldwide is increasing year by year. Sensors play an important role in the development of biomedical devices. The development of efficient, stable, and inexpensive glucose sensors for the continuous monitoring of blood glucose levels has received widespread attention because they can provide reliable data for diabetes prevention and diagnosis. Electrospun nanofibers are new kinds of functional nanocomposites that show incredible capabilities for high-level biosensing. This article reviews glucose sensors based on electrospun nanofibers. The principles of the glucose sensor, the types of glucose measurement, and the glucose detection methods are briefly discussed. The principle of electrospinning and its applications and advantages in glucose sensors are then introduced. This article provides a comprehensive summary of the applications and advantages of polymers and nanomaterials in electrospun nanofiber-based glucose sensors. The relevant applications and comparisons of enzymatic and non-enzymatic nanofiber-based glucose sensors are discussed in detail. The main advantages and disadvantages of glucose sensors based on electrospun nanofibers are evaluated, and some solutions are proposed. Finally, potential commercial development and improved methods for glucose sensors based on electrospinning nanofibers are discussed.

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Section: Electrospun Nanofiber-based Enzymatic and Non-enzymatic Gluc...mentioning

confidence: 99%

Section: Principles Of Glucose Sensorsmentioning

confidence: 99%

Electrospun nanofiber-based glucose sensors for glucose detection

Zhang

Liu

et al. 2022

Front. Chem.

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Biosensor and Healthcare Vis-à-Vis Cloud Computing and IoT: Towards Sophisticated Healthcare Development—An Overview

Paul

2021

Studies in Systems, Decision and Control

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An integrated microfluidic-based biosensor using a magnetically controlled MNPs-enzyme microreactor to determine cholesterol in serum with fluorometric detection

2023

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This work provides a microfluidic-based biosensor to determine total cholesterol in serum based on integrating the reaction/detection zone of a microfluidic chip of a magnetically retained enzyme microreactor (MREµR) coupled with the remote fluorometric detection through a bifurcated fiber-optic bundle (BFOB) connected with a conventional spectrofluorometer. The method is based on developing the enzymatic hydrolysis and oxidation of cholesterol at microscale size using both enzymes (cholesterol esterase (ChE) and cholesterol oxidase (ChOx)) immobilized on magnetic nanoparticles (MNPs). The biocatalyst reactions were followed by monitoring the fluorescence decreasing by the naphtofluorescein (NF) oxidation in the presence of the previous H2O2 formed. This microfluidic biosensor supposes the physical integration of a minimal MREµR as a bioactive enzyme area and the focused BFOB connected with the spectrofluorometer detector. The MREµR was formed by a 1 mm length of magnetic retained 2:1 ChE-MNP/ChOx-MNP mixture. The dynamic range of the calibration graph was 0.005–10 mmol L−1, expressed as total cholesterol concentration with a detection limit of 1.1 µmol L−1 (r2 = 0.9999, sy/x = 0.03, n = 10, r = 3). The precision expressed as the relative standard deviation (RSD%) was between 1.3 and 2.1%. The microfluidic-based biosensors showed a sampling frequency estimated at 30 h−1. The method was applied to determine cholesterol in serum samples with recovery values between 94.8 and 102%. The results of the cholesterol determination in serum were also tested by correlation with those obtained using the other two previous methods. Graphical abstract

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The imperative role of polymers in enzymatic cholesterol biosensors- an overview

Cited by 5 publications

References 196 publications

Electrospun nanofiber-based glucose sensors for glucose detection

Electrospun nanofiber-based glucose sensors for glucose detection

Biosensor and Healthcare Vis-à-Vis Cloud Computing and IoT: Towards Sophisticated Healthcare Development—An Overview

An integrated microfluidic-based biosensor using a magnetically controlled MNPs-enzyme microreactor to determine cholesterol in serum with fluorometric detection

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