The impaired development of sheep ICSI derived embryos is not related to centriole dysfunction

Ressaissi, Yosra; Anzalone, Debora Agata; Palazzese, Luca; Czernik, Marta; Loi, Pasqualino

doi:10.1016/j.theriogenology.2020.10.008

Cited by 10 publications

(6 citation statements)

References 24 publications

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“…Tracking the changes in epigenomic and transcriptomic signatures of in vitro‐matured ewe oocytes and corresponding IVF‐derived embryos that have undergone the exposure to a variety of nutritional and metabolic stressors might contribute to a better understanding the molecular interplay between pro‐ and antioxidative events and the relative abundances of DNMT mRNA transcripts. Exploring the above‐indicated interplay might result in the improved developmental capabilities and quality parameters noticed for ovine and other mammalian embryos produced not only by gamete coincubation‐mediated IVF (Anzalone et al, 2021; Gorczyca et al, 2022) or intracytoplasmic sperm injection (ICSI)‐mediated IVF (Ramírez‐Agámez et al, 2023; Ressaissi et al, 2021; Shirazi et al, 2018), but also by somatic cell cloning (Fang et al, 2020; Gorczyca et al, 2021; Nadri et al, 2022; Wiater et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Global DNA methylation, DNA methyltransferase and stress‐related gene expression in ovine oocytes and embryos after exposure to metabolic stressors

Nandi

Tripathi

Singh

et al. 2023

Reprod Domestic Animals

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DNA methylation, considered the most prominent epigenetic mark was important for the gene regulation in embryonic development. The present study aimed at evaluating the effects of metabolic stressors [Non‐esterified fatty acid (NEFA), β‐hydroxy‐butyric acid (BHB), ammonia and urea] exposure during the in vitro ovine oocyte maturation, global DNA methylation, DNA methyltransferase and stress‐related gene expression. Colorimetric analysis of global DNA methylation and the expression of the DNA methyltransferase genes (DNMT1, DNMT3A, and DNMT3B) were assessed in the matured oocytes, 2‐cell embryos and blastocysts produced in vitro from oocytes exposed with the metabolic stressors during 24 h of the in vitro maturation (IVM). Further, the mRNA expression of the stress‐related genes (SOD1, SOD2) in the matured oocytes, 2‐cell embryos and blastocysts produced was assessed. Significant difference in global DNA methylation levels between all the treatments tested was observed when compared with control in oocytes, two‐cell embryos and blastocysts. Elevated concentration of metabolic stressors resulted in increased expressions of several stress‐related genes, i.e., SOD1, SOD2 and in mRNA expression of DNA methyltransferase genes. The present study is the first to report that the DNA methylation was sensitive to the effects of the metabolic stressors in ovine oocytes/embryos. The aberrant expressions of genes during oocyte development targeted in the present study can provide evidence for the early embryo developmental arrest and blastocysts quality. These results highlighted the sensitivity of the early embryogenesis and more precisely of the reprogramming period to metabolites challenges, in a realistic situation of elevated concentration of metabolic stressors.

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Section: Discussionmentioning

confidence: 99%

Global DNA methylation, DNA methyltransferase and stress‐related gene expression in ovine oocytes and embryos after exposure to metabolic stressors

Nandi

Tripathi

Singh

et al. 2023

Reprod Domestic Animals

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“…Alternatively, ICSI-based IVF can be achieved with the aid of the freeze-dried (lyophilized) and subsequently rehydrated sperm cells for microsurgical deposition into a cytoplasm of MII-stage oocytes [34,72]. For ICSI-fertilized oocytes, the artificial activation of their embryonic developmental program, which has been prompted by extrinsic factors (e.g., electrostimulation, ionomycin-mediated intraooplasmic iontophoretic transport of extracellular calcium sources) also plays a pivotal role [109,110]. Furthermore, epigenomic reprogrammability of oocyte-and spermatozoon-inherited chromosomes brings about the pre-and postimplantation developmental competences of IVFor ICSI-derived embryos [111][112][113].…”

Section: The Applicability Of Ex Situ Conservation Methods To Modern ...mentioning

confidence: 99%

Creating Ex Situ Protected Bioreservoirs as a Powerful Strategy for the Reproductive Biotechnology-Mediated Rescue of Threatened Polish Livestock Breeds

2023

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The current article presents the state of the art of the creation of bioreservoirs of cryopreserved somatic and stem cell lines and cryopreserved or lyophilized germplasm-based resources of selected farm animal species. It also presents the agricultural, biological and biotechnological determinants, and showcases the importance of the National Research Institute of Animal Production (NRIAP) in Poland in this process. The aforementioned bioreservoirs serve as an innovative research tool used for cryogenically or lyophilizogenically assisted and species-specific ex situ conservation. The latter ensures, in the long term, not only restitution, but also perpetuation of sustainable biodiversity that underlies genotypic and phenotypic, intra- and inter-population variability within pure-breeding herds of the national conserved livestock breeds.

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“…Embryo cryopreservation [60]; sperm cryopreservation [180,181]; testis xenotransplantation from humans [182] and several species, like pigs and goats [183]; germ cell transplantation [159,184]; ROSI [177,185]; First embryonic stem cells (ESCs) isolation [186,187]; artificial gamete derivation up to offspring [188]; Spermatogenesis in vitro [189]; iPS cell derivation [190]; Alginate encapsulation [191]; in vitro expansion of SSCs [192] Rat germ cell transplantation [159]; MESA [129]; iPS cell derivation [193] Hamster IVF [59]; ICSI [31] Rabbit AI first experiences [36]; Sperm cryopreservation [180]; ET/IVF first experiences [48]; ES cell derivation [194] Cats Sperm cryopreservation [180] Dog AI first experiences [36]; Sperm cryopreservation [180]; MESA [128] Pigs Germ Cell Transplantation [195]; Sperm cryopreservation (high difficulty) [72] Horse AI first experiences [36]; Sperm cryopreservation [180]; ICSI [196] Bulls Sperm cryopreservation [47,180]; sexed semen [41]; ET [48]; in vitro expansion of SSCs [197]; SSC cryopreservation [198]; SSC transplantation…”

Section: Mousementioning

confidence: 99%

Bridging the Gap: Animal Models in Next-Generation Reproductive Technologies for Male Fertility Preservation

Aponte,

Gutierrez-Reinoso,

Garcia-Herreros

2023

Life

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This review aims to explore advanced reproductive technologies for male fertility preservation, underscoring the essential role that animal models have played in shaping these techniques through historical contexts and into modern applications. Rising infertility concerns have become more prevalent in human populations recently. The surge in male fertility issues has prompted advanced reproductive technologies, with animal models playing a pivotal role in their evolution. Historically, animal models have aided our understanding in the field, from early reproductive basic research to developing techniques like artificial insemination, multiple ovulation, and in vitro fertilization. The contemporary landscape of male fertility preservation encompasses techniques such as sperm cryopreservation, testicular sperm extraction, and intracytoplasmic sperm injection, among others. The relevance of animal models will undoubtedly bridge the gap between traditional methods and revolutionary next-generation reproductive techniques, fortifying our collective efforts in enhancing male fertility preservation strategies. While we possess extensive knowledge about spermatogenesis and its regulation, largely thanks to insights from animal models that paved the way for human infertility treatments, a pressing need remains to further understand specific infertility issues unique to humans. The primary aim of this review is to provide a comprehensive analysis of how animal models have influenced the development and refinement of advanced reproductive technologies for male fertility preservation, and to assess their future potential in bridging the gap between current practices and cutting-edge fertility techniques, particularly in addressing unique human male factor infertility.

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The impaired development of sheep ICSI derived embryos is not related to centriole dysfunction

Cited by 10 publications

References 24 publications

Global DNA methylation, DNA methyltransferase and stress‐related gene expression in ovine oocytes and embryos after exposure to metabolic stressors

Global DNA methylation, DNA methyltransferase and stress‐related gene expression in ovine oocytes and embryos after exposure to metabolic stressors

Creating Ex Situ Protected Bioreservoirs as a Powerful Strategy for the Reproductive Biotechnology-Mediated Rescue of Threatened Polish Livestock Breeds

Bridging the Gap: Animal Models in Next-Generation Reproductive Technologies for Male Fertility Preservation

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