For more than sixty years, textile vascular grafts have saved millions human lives, but achieving attributes of a native artery still remains a challenge, especially in terms of parietal distensibility, commonly called compliance, necessary to transform intermittent blood ejection into steady and continuous flows. The graft compliance is, in part, related to the fabric contexture and the kind of yarns used in relation with the prosthetic finishing treatments. The objective of the present paper was to evaluate the mechanical behavior of prosthetic fabrics, made with various polyester multifilament yarns, subjected to fatigue stresses under realistic test conditions performed in vitro. For this purpose, cyclic tests have been applied to prosthetic tubes by using both mechanical testing machine and test bench specially developed for simulating the mechanical aging induced by water pressure on the prosthetic wall. This instrument allows the injection of pulsatile water adjustable in frequency and pressure in vascular grafts previously sealed by a rubber bladder while simultaneously monitoring the prosthesis wall distention by a high resolution displacement transducer linked to data acquisition software. Compliance curves thus made it possible to identify the highdistensibility prosthetic structures whose cyclic dilation could be maintained almost stable throughout the fatigue tests.