The impacts of biosecurity measures on Campylobacter contamination in broiler houses and slaughterhouses in the Netherlands: A simulation modelling approach

Horvat, Andrijana; Luning, Pieternel A.; DiGennaro, Catherine; Rommens, Edien; Daalen, Els van; Koene, Miriam; Jalali, Mohammad S.

doi:10.1016/j.foodcont.2022.109151

Cited by 6 publications

(7 citation statements)

References 45 publications

(63 reference statements)

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“…All chickens sampled on this farm had negative caecal growth of Campylobacter but exhibited positive growth on the surface of neck skins from the same flock post-slaughter. The observed negative growth in the caeca could be explained by good management practices and biosecurity performed on the farm 63 , 64 .…”

Section: Discussionmentioning

confidence: 92%

Genomic tailoring of autogenous poultry vaccines to reduce Campylobacter from farm to fork

Calland,

Pesonen,

Mehat

et al. 2024

npj Vaccines

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Campylobacter is a leading cause of food-borne gastroenteritis worldwide, linked to the consumption of contaminated poultry meat. Targeting this pathogen at source, vaccines for poultry can provide short-term caecal reductions in Campylobacter numbers in the chicken intestine. However, this approach is unlikely to reduce Campylobacter in the food chain or human incidence. This is likely as vaccines typically target only a subset of the high genomic strain diversity circulating among chicken flocks, and rapid evolution diminishes vaccine efficacy over time. To address this, we used a genomic approach to develop a whole-cell autogenous vaccine targeting isolates harbouring genes linked to survival outside of the host. We hyper-immunised a whole major UK breeder farm to passively target offspring colonisation using maternally-derived antibody. Monitoring progeny, broiler flocks revealed a near-complete shift in the post-vaccination Campylobacter population with an ~50% reduction in isolates harbouring extra-intestinal survival genes and a significant reduction of Campylobacter cells surviving on the surface of meat. Based on these findings, we developed a logistic regression model that predicted that vaccine efficacy could be extended to target 65% of a population of clinically relevant strains. Immuno-manipulation of poultry microbiomes towards less harmful commensal isolates by competitive exclusion, has major potential for reducing pathogens in the food production chain.

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Section: Discussionmentioning

confidence: 92%

Genomic tailoring of autogenous poultry vaccines to reduce Campylobacter from farm to fork

Calland,

Pesonen,

Mehat

et al. 2024

npj Vaccines

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“…High biosecurity measures are required on farms to prevent the introduction of Campylobacter into the flock and to protect flocks from colonisation [33]. Horvat et al ( 2022) used a simulation model and showed that insect control had the strongest impact of all tested intervention measures to prevent Campylobacter contamination by reducing the percentage of highly contaminated (>1000 cfu/g) neck samples from 13% to 8% [34]. The low contamination levels of Campylobacter in broilers at primary production seem to have a major impact on reducing Campylobacter enteritis cases, as Foddai et al (2022) investigated that targeted management measures on high-risk farms could significantly reduce the risk of Campylobacter infection for the consumer [35].…”

Section: Discussionmentioning

confidence: 99%

Process Hygiene Criterion for Campylobacter and Number of Campylobacter Enteritis Cases in Northwest Germany

Nolte,

Spieß,

Jacobs

et al. 2024

Foods

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Campylobacteriosis is the most commonly reported bacterial foodborne disease in the European Union. Its transmission is often associated with the consumption of poultry meat. In 2018, Regulation (EC) No. 2017/1495 introduced a process hygiene criterion and with this, the testing requirements for Campylobacter. The results of microbiological testing for Campylobacter of chicken carcass neck skin samples from several slaughter lines in Northwest Germany collected by the food business operators and contamination levels (cfu/g Campylobacter) of these samples were analysed from 2018 to 2021. Classification into three different categories was made based on contamination levels. The proportion of highly contaminated (category three) neck samples (>1000 cfu/g) decreased from 2018 to 2021. Our analysis showed a relationship between the number of neck samples with high Campylobacter contamination levels (>1000 cfu/g) and human cases in Northwest Germany. Spearman’s rank test (p < 0.01) showed a higher correlation in 2018 (0.66) and 2019 (0.58) compared to 2020 and 2021. Campylobacter enteritis cases in Northwest Germany stayed at a low level in 2020 and 2021. It remains unclear whether the decrease in reported Campylobacter enteritis cases is related to a decrease in Campylobacter levels on chicken carcasses or due to other reasons like underreporting during the COVID-19 pandemic, and therefore must be investigated in further analyses.

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“…Preventing fly entry during summer leads to a decrease in the prevalence of Campylobacter positive flocks at slaughter (Hald et al, 2007). Generally, insect control interventions reduce the peak percentage of contaminated chickens and neck samples of chicken carcasses (Horvat et al, 2022). The problem can be solved by regular removal of carcasses, repairing all leaking water lines and keeping the manure dry, and relocating used litter from the farm.…”

Section: Discussionmentioning

confidence: 99%

“…Vectors for transmission of the pathogen potentially could be farmers (boots, clothes, and equipment), rodents, and insects (Newell and Fearnley, 2003;Battersby et al, 2016;Royden et al, 2021). Studies showed that multiple interventions, hygiene, biosecurity measures, and additional and complementary interventions (probiotics, vaccination) help in controlling the incidence of Campylobacter infection in poultry broiler flocks (Gibbens et al, 2001;Newell et al, 2011;Horvat et al, 2022). A linear relationship between the flock prevalence and incidence of campylobacteriosis in humans is confirmed (Rosenquist et al, 2003), so reducing the prevalence of positive flocks should contribute to the reduction of disease in humans (Newell and Fearnley, 2003;EFSA, 2011).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Biosecurity Measures May Contribute to the Reduction of Campylobacter Incidence in Slaughterhouses

Maletić,

Kureljušić,

Katanić

2023

Meat Technology

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As a preventive measure, biosecurity is the first line of defense against many pathogens. Applied biosecurity measures can reduce the prevalence of Campylobacter infection in commercial broiler populations. A systematic evaluation, encompassing at least annual monitoring of applied biosecurity measures and on-farm prevalence of Campylobacter infection is highly recommended.This study was performed on three broiler farms with the aim to assess the effectiveness of the biosecurity measures applied. Broiler farms included in the study previously had problems with Campylobacter infections, and therefore, after the intervention through a risk-based scoring system and bacteriological testing of samples from the farm and the corresponding carcasses in the slaughterhouse, several biosecurity measures were implemented. Obtained results showed that after the intervention, farms increased their external biosecurity by 16.34%, internal biosecurity by 22%, and overall biosecurity by 18.34%. The major interventions concerned the removal of manure and carcasses, all improved measures taken for feed and drinking water, and measures in the subcategory of cleaning and disinfection protocols carried out between two production cycles. After the improvements, during the screening process on the farms, Campylobacter was not isolated from pooled fecal samples in any of the broiler houses. This indicates that at least six houses (two houses per farm) were Campylobacter-negative at broiler slaughter age. In pooled neck skin samples originating from studied farms, Campylobacter was not isolated after the improved measures were implemented.The results showed that the assessment of biosecurity protocols on broiler farms is a useful tool, and Campylobacter can serve as a biomarker for the efficiency of the implemented biosecurity protocols.

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The impacts of biosecurity measures on Campylobacter contamination in broiler houses and slaughterhouses in the Netherlands: A simulation modelling approach

Cited by 6 publications

References 45 publications

Genomic tailoring of autogenous poultry vaccines to reduce Campylobacter from farm to fork

Genomic tailoring of autogenous poultry vaccines to reduce Campylobacter from farm to fork

Process Hygiene Criterion for Campylobacter and Number of Campylobacter Enteritis Cases in Northwest Germany

Enhanced Biosecurity Measures May Contribute to the Reduction of Campylobacter Incidence in Slaughterhouses

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