The impacts and mechanisms of dietary proteins on glucose homeostasis and food intake: a pivotal role of gut hormones

Zhang, Mingkai; Zhu, Ling; Wu, Gangcheng; Zhang, Hui; Wang, Xingguo; Qi, Xiguang

doi:10.1080/10408398.2023.2256400

Cited by 4 publications

(5 citation statements)

References 106 publications

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“…Figure S3 shows that the relative GLP-1 levels induced by LRW (272.37% ± 45.96% vs 201.67% ± 10.97%) and FLR (170.20% ± 27.83% vs 131.67% ± 5.51%) all decreased ( p < 0.05) in the presence of 4-AMBA, suggesting that PepT1 activation might be involved in the GLP-1-secreting peptide-induced GLP-1 release in NCI-H716 cells. Interestingly, the relative GLP-1 levels were still higher than that of the control group ( p < 0.05), suggesting other sensing receptors, such as the calcium sensing receptor (CaSR) and G-protein receptor-93 (GPR93), may also be involved in GLP-1-secreting peptide-triggered GLP-1 secretion . For instance, GLP-1-secreting peptides SRYPS and LITVQTM stimulated GLP-1 secretion by activating CaSR and GPR93 .…”

Section: Resultsmentioning

confidence: 96%

“…Nowadays, studies have demonstrated that many protein hydrolysates exhibit the capacity to stimulate GLP-1 secretion, while only a few GLP-1-secreting peptides have been obtained from these protein hydrolysates. 11 Figure 4B shows the GLP-1 levels induced by these four tripeptides (1 mg/mL) identified from PPHs. Among these, FLR and LRW increased the GLP-1 levels by 170.20% ± 27.83% and 272.37% ± 45.96%, respectively.…”

Section: Active Verification Of Glp-1-secreting Peptidesmentioning

confidence: 99%

“…Interestingly, the relative GLP-1 levels were still higher than that of the control group (p < 0.05), suggesting other sensing receptors, such as the calcium sensing receptor (CaSR) and Gprotein receptor-93 (GPR93), may also be involved in GLP-1secreting peptide-triggered GLP-1 secretion. 11 For instance, GLP-1-secreting peptides SRYPS and LITVQTM stimulated GLP-1 secretion by activating CaSR and GPR93. 32 Similarly, several GLP-1-secreting peptides, such as γ-Glu-Phe, γ-Glu-Met, and γ-Glu-Val, also induced GLP-1 release by CaSR activation.…”

Section: Active Verification Of Glp-1-secreting Peptidesmentioning

confidence: 99%

“…10 However, GLP-1-secreting peptides that were identified from food proteins are still very scarce, and their structure−activity relationship has not been well-established. 11 More importantly, the traditional approach used to identify GLP-1-secreting peptides by activity-driven separation and purification was time-consuming and laborious. 12 Interestingly, the novel bioinformational method based on virtual screening exhibited convenience and efficiency in bioactive peptide identification.…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, two GLP-1-secreting peptides PFL and RVASMASEKM were also obtained from egg white digest . However, GLP-1-secreting peptides that were identified from food proteins are still very scarce, and their structure–activity relationship has not been well-established . More importantly, the traditional approach used to identify GLP-1-secreting peptides by activity-driven separation and purification was time-consuming and laborious .…”

Section: Introductionmentioning

confidence: 99%

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Virtual Screening of GLP-1-Secreting Peptides from Pea Protein Hydrolysates via Peptide Transporter 1 (PepT1) Activation-Based Molecular Docking

Zhang,

Zhu,

Zhang

et al. 2024

J. Agric. Food Chem.

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Dietary proteins regulate glucose homeostasis via intestinal protein sensing-induced glucagon-like peptide 1 (GLP-1) secretion. However, the reported GLP-1-secreting peptides derived from dietary proteins are few, and studies regarding GLP-1secreting peptide identification by traditional separation and purification methods are laborious. Herein, we have rapidly virtualscreened two GLP-1 secreting peptides from pea protein hydrolysates (PPHs) by peptidomic analysis and molecular docking with peptide transporter 1 (PepT1). PPH-stimulated GLP-1 secretion decreased after adding the PepT1 antagonist 4-aminobenzoic acid (4-AMBA), indicating that PepT1 activation was involved in PPH-induced GLP-1 secretion in NCI-H716 cells. Subsequently, 307 tripeptides in PPHs were obtained through peptidomic analysis. Among them, two GLP-1-secreting peptides, FLR and LRW, were identified via PepT1 activation-based molecular docking. FLR and LRW (1 mg/mL) increased GLP-1 levels to 170.20% ± 27.83% and 272.37% ± 45.96%, respectively (p < 0.05). More importantly, molecular docking implied that the interactions between peptides and the active center of PepT1 (especially Glu595, Asn329, and Asn171 in the N-pocket and Arg27 in the C-pocket) were crucial for peptide activity in stimulating GLP-1 secretion. Our study suggested that the combination of peptidomics and PepT1 activationbased molecular docking is a promising approach for identification of GLP-1-secreting peptides.

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Section: Resultsmentioning

confidence: 96%

Section: Active Verification Of Glp-1-secreting Peptidesmentioning

confidence: 99%

Section: Active Verification Of Glp-1-secreting Peptidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Virtual Screening of GLP-1-Secreting Peptides from Pea Protein Hydrolysates via Peptide Transporter 1 (PepT1) Activation-Based Molecular Docking

Zhang,

Zhu,

Zhang

et al. 2024

J. Agric. Food Chem.

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Multiple health outcomes associated with algae and its extracts supplementation: An umbrella review of systematic reviews and meta‐analyses

Wang,

Min,

Zhou

et al. 2024

Phytotherapy Research

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Algae and its extracts, widely consumed as functional foods, offer numerous health benefits; however, a comprehensive systematic summary of clinical evidence is currently lacking. The study was to assess the available evidence and provide an accurate estimate of the overall effects of algae and its extracts supplementation on various health outcomes. The comprehensive searches in PubMed, Scopus, Embase, Web of Science, and the Cochrane Library until December 22, 2023 were implemented. The random‐effects model was employed to pool the overall effect sizes (ESs) and the corresponding 95% confidence intervals (CIs) using Stata software. Moreover, detecting the methodological quality and evidence level of the eligible studies were employed by A Measurement Tool to Assess Systematic Review 2 (AMSTAR2) and the Grading of Recommendations Assessment Development and Evaluation. Ultimately, 25 articles covering 133 health outcomes were included in this umbrella review. The pooled results demonstrated that the algae and its extracts could significantly decrease body weight (ES = −1.65; 95% CI: −1.97, −1.34; p < 0.001), body mass index (BMI) (ES = −0.42; 95% CI: −0.78, −0.07; p = 0.020), waist circumference (WC) (ES = −1.40; 95% CI: −1.40, −1.39; p < 0.001), triglyceride (TG) (ES = −1.38; 95% CI: −2.15, −0.62; p < 0.001), total cholesterol (TC) (ES: −1.40; 95% CI: −2.09, −0.72; p < 0.001), very low‐density lipoprotein cholesterol (VLDL‐C) (ES = −7.85; 95% CI: −8.55, −7.15; p < 0.001), fasting blood glucose (ES = −2.68; 95% CI: −4.57, −0.79; p = 0.005), glycated hemoglobin (HbA1c) (ES = −0.15; 95% CI: −0.24, −0.07; p < 0.001), systolic blood pressure (ES = −3.21; 95% CI: −5.25, −1.17; p = 0.002), diastolic blood pressure (ES = −3.84; 95% CI: −7.02, −0.65; p = 0.018), alanine transaminase (ES = −0.42; 95% CI: −0.70, −0.14; p = 0.003), and alkaline phosphatase (ES = −0.54; 95% CI: −0.99, −0.10; p = 0.017). Due to the limited number of studies, no benefit was displayed on markers of inflammation and oxidative stress. Considering the suboptimal quality of studies and the insufficient articles pertaining to certain outcomes, further well‐designed research is imperative to substantiate the observed findings.

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Pea protein hydrolysate stimulates GLP-1 secretion in NCI-H716 cells via simultaneously activating the sensing receptors CaSR and PepT1

Zhang,

Zhu,

Zhang

et al. 2024

Food Funct.

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The impacts and mechanisms of dietary proteins on glucose homeostasis and food intake: a pivotal role of gut hormones

Cited by 4 publications

References 106 publications

Virtual Screening of GLP-1-Secreting Peptides from Pea Protein Hydrolysates via Peptide Transporter 1 (PepT1) Activation-Based Molecular Docking

Virtual Screening of GLP-1-Secreting Peptides from Pea Protein Hydrolysates via Peptide Transporter 1 (PepT1) Activation-Based Molecular Docking

Multiple health outcomes associated with algae and its extracts supplementation: An umbrella review of systematic reviews and meta‐analyses

Pea protein hydrolysate stimulates GLP-1 secretion in NCI-H716 cells via simultaneously activating the sensing receptors CaSR and PepT1

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