The Impact on Antioxidant Enzyme Activity and Related Gene Expression Following Adult Zebrafish (Danio rerio) Exposure to Dimethyl Phthalate

Cong, Bin; Liu, Cong; Wang, Lujie; Chai, Yingmei

doi:10.3390/ani10040717

Cited by 36 publications

(15 citation statements)

References 54 publications

(61 reference statements)

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“…The SOD activity in untreated samples showed a declining trend with a significant decrease of ~1.2-fold at 70 ppm ( p < 0.001) which continued to decline by ~1.5-fold at 110 ppm, which may be attributed to a high level of oxidative stress generated beyond the preventive potential of the existing SOD level [ 37 ]. Similar decreasing trends in SOD activities are reported wherein zebrafish larvae were exposed to increasing dimethyl phthalate concentration [ 38 ] or pesticide endosulfan [ 39 ]. In treated samples, SOD activity was found to be relatively closer to the control for initial concentrations and marginal decline at the maximum concentration, i.e., 110 ppm ( p < 0.01).…”

Section: Resultssupporting

confidence: 77%

Toxicity Mitigation of Textile Dye Reactive Blue 4 by Hairy Roots of Helianthus annuus and Testing Its Effect in In Vivo Model Systems

Tungare

Shahu

Zambare

et al. 2022

BioMed Research International

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An anthraquinone textile dye, Reactive Blue 4 (RB4), poses environmental health hazards. In this study, remediation of RB4 (30-110 ppm) was carried out by hairy roots (HRs). UV-visible spectroscopy and FTIR analysis showed that the dye undergoes decolourization followed by degradation. In addition, toxicity and safety analyses of the bioremediated dye were performed on Allium cepa and zebrafish embryos, which revealed lesser toxicity of the bioremediated dye as compared to untreated dye. For Allium cepa, the highest concentration, i.e., 110 ppm of the treated dye, showed less chromosomal aberrations with a mitotic index of 8.5 ± 0.5 , closer to control. Two-fold decrease in mortality of zebrafish embryos was observed at the highest treated dye concentration indicating toxicity mitigation. A higher level of lipid peroxidation (LPO) was recorded in the zebrafish embryo when exposed to untreated dye, suggesting a possible role of oxidative stress-inducing mortality of embryos. Further, the level of LPO was significantly normalized along with the other antioxidant enzymes in embryos after dye bioremediation. At lower concentrations, mitigated samples displayed similar antioxidant activity comparable to control underlining the fact that the dye at lesser concentration can be more easily degraded than the dye at higher concentration.

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Section: Resultssupporting

confidence: 77%

Toxicity Mitigation of Textile Dye Reactive Blue 4 by Hairy Roots of Helianthus annuus and Testing Its Effect in In Vivo Model Systems

Tungare

Shahu

Zambare

et al. 2022

BioMed Research International

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“…In the larvae exposed to ( S )‐VEN, there was a decrease in SOD activity at 30 and 3000 μg/L compared to the control (Supporting Information,Table D). This decrease in SOD activity is associated with the decrease in previously observed ROS levels, because the SOD plays an important role in the first defence system (Cong et al, 2020) when ROS levels increased. Catalase activity increased significantly in larvae exposed to 30 μg/L of ( R,S )‐VEN compared to the control but no differences were observed for ( R )‐VEN and ( S )‐VEN.…”

Section: Resultsmentioning

confidence: 81%

Enantioselective Ecotoxicity of Venlafaxine in Aquatic Organisms: Daphnia and Zebrafish

Ribeiro

Félix

Ribeiro

et al. 2022

Enviro Toxic and Chemistry

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Venlafaxine is a chiral antidepressant detected in aquatic compartments. It was recently included in the 3rd Watch List from the European Union. The present study aimed to investigate venlafaxine toxicity effects, targeting possible enantioselective effects, using two aquatic organisms, daphnia (Daphnia magna) and zebrafish (Danio rerio). Specimens were exposed to both racemate, (R,S)‐venlafaxine (VEN), and to pure enantiomers. Acute assays with daphnia showed that up to 50 000 μg/L of the (R,S)‐VEN induced no toxicity. Organisms were also exposed to sublethal concentrations (25–400 μg/L) of (R,S)‐, (R)‐ and (S)‐VEN, for 21 days. No significant effects on mortality, age at first reproduction, and size of the first clutch were observed. However, a decrease in fecundity was observed for both enantiomers at the highest concentration. Regarding zebrafish, the effects of venlafaxine on mortality, embryo development, behavior, biochemistry, and melanin pigmentation were investigated after 96 h of exposure to the range of 0.3–3000 μg/L. (R)‐VEN significantly increased the percentage of malformations in comparison with (S)‐VEN. Behavior was also enantiomer dependent, with a decrease in the total distance moved and an increase in avoidance behavior observed in organisms exposed to (R)‐VEN. Despite the biochemical variations, no changes in redox homeostasis were observed. (R)‐VEN also led to an increase in zebrafish pigmentation. The different susceptibility to venlafaxine and enantioselective effects were observed in zebrafish. Our results suggest that at environmental levels (R,S)‐VEN and pure enantiomers are not expected to induce harmful effects in both organisms, but (R)‐VEN increased malformations in zebrafish larvae, even at reported environmental levels. These results highlight the importance of including enantioselective studies for an accurate risk assessment of chiral pollutants. Environ Toxicol Chem 2022;41:1851–1864. © 2022 SETAC

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“…We observed downregulation of sod1 , sod2 and sod3 by all the doses of DINCH. It has previously been indicated that these genes were affected by several plasticizers such as DEHP, dimethyl phthalate (DMP), and butyl benzyl phthalate (BBP) in zebrafish and medaka fish ( Cong et al., 2020 ; Yang et al., 2018 ; Zhang et al., 2014 ). It has also been shown that SOD1 enzyme activity was overexpressed in response to subacute exposure to DINCH at post-natal day 21 of dams, while it was repressed upon in utero exposure to DINCH at post-natal day 60 in a dose-dependent manner ( Campioli et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) alters transcriptional profiles, lipid metabolism and behavior in zebrafish larvae

Saad

Bereketoğlu

Pradhan

2021

Heliyon

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Plasticizers are commonly used in different consumer goods and personal care products to provide flexibility, durability and elasticity to polymers. Due to their reported toxicity, the use of several plasticizers, including phthalates has been regulated and/or banned from the market. Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) is an alternative plasticizer that was introduced to replace toxic plasticizers. Increasing global demand and lack of toxicity data and safety assessment of DINCH have raised the concern to human and animal health. Hence, in the present study, we investigated the adverse effects of DINCH (at concentrations ranging from 0.01 to 10 μM) in early developmental stages of zebrafish using different endpoints such as hatching rate, developmental abnormalities, lipid content, behavior analysis and gene expression. We found that DINCH caused hatching delay in a dose-dependent manner and altered the expression of genes involved in stress response. Lipid staining using Oil Red O stain showed a slight lipid accumulation around the yolk, brain, eye and neck with increasing concentration. Genes associated with lipid transport such as fatty acid synthesis, β-oxidation, elongation, lipid transport were significantly altered by DINCH. Genes involved in cholesterol biosynthesis and homeostasis were also affected by DINCH indicating possible developmental neurotoxicity. Behavioral analysis of larvae demonstrated a distinct locomotor activity upon exposure to DINCH. The present data shows that DINCH could induce physiological and metabolic toxicity to aquatic organisms. Hence, further analyses and environmental monitoring of DINCH should be conducted to determine its safety and toxicity levels.

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The Impact on Antioxidant Enzyme Activity and Related Gene Expression Following Adult Zebrafish (Danio rerio) Exposure to Dimethyl Phthalate

Cited by 36 publications

References 54 publications

Toxicity Mitigation of Textile Dye Reactive Blue 4 by Hairy Roots of Helianthus annuus and Testing Its Effect in In Vivo Model Systems

Toxicity Mitigation of Textile Dye Reactive Blue 4 by Hairy Roots of Helianthus annuus and Testing Its Effect in In Vivo Model Systems

Enantioselective Ecotoxicity of Venlafaxine in Aquatic Organisms: Daphnia and Zebrafish

Di(isononyl) cyclohexane-1,2-dicarboxylate (DINCH) alters transcriptional profiles, lipid metabolism and behavior in zebrafish larvae

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