The impact of whole human blood on the kinetic inertness of platinum(iv) prodrugs – an HPLC-ICP-MS study

Abstract

The reductive biotransformation of platinum(iv) prodrugs was investigated in whole human blood by HPLC-ICP-MS.

Serum-binding properties of isosteric ruthenium and osmium anticancer agents elucidated by SEC–ICP–MS

“…In addition, different chromatographic separation techniques were employed in combination with ICP–MS, including hydrophilic interaction liquid chromatography (HILIC) [ 19 ], reversed-phase (RP) chromatography [ 13 , 18 , 21 , 22 ], and size-exclusion chromatography (SEC) [ 12 , 14 – 17 , 23 , 24 ]. Recently, LC–ICP–MS studies were successfully applied to study the intracellular distribution of intact cisplatin [ 25 ] or platinum(IV) drug candidates in fractionated whole blood [ 26 ].…”

Heart-cut 2DSEC-RP-LC-ICP-MS as a screening tool in metal-based anticancer research

“…Reversed phase separations using polar embedded groups or other functionalized C18 material had been shown in the past to be suitable for the selective separation of oxaliplatin and its hydrolysis products. 19,20 Using 1D RP-LC-ICP-MS or 2D UHPLC SEC-RP-ICP-MS for the analysis of intact oxaliplatin in 15 mM NaCl excellent separation of oxaliplatin from hydrolysis products could be observed (Fig. 2).…”

“…The reversed phase separation was carried out using either a fully wettable C18 material or pen-tauorophenyl phases allowing stacked injections (high volume) of metal-based drugs showing medium to high retention on the reversed phase. 19,20 Due to the use of quaternary pumps, 3 mm-particle RP-LC stationary phases were selected, as imposed by the pressure limit of the pump. Reversed phase separations using polar embedded groups or other functionalized C18 material had been shown in the past to be suitable for the selective separation of oxaliplatin and its hydrolysis products.…”

Effect of pH and mobile phase additives on the chromatographic behaviour of an amide‐embedded stationary phase: Cyanocobalamin and its diaminemonochloro‐platinum(II) conjugate as a case study

“…Cellular uptake of cisplatin conjugates is typically followed via detection of their metal constituents by ICP‐MS . Yet, a mass spectrometric technique providing detailed molecular information, like MALDI‐MS, has been recently proposed for the structural characterization of CNCbl and CNCbl –Pt(II) drug (e.g.…”

“…Definite evidences have been provided that the cyanide-bridged conjugate, [CNCbl-cis-DAMCP] + , formed between the cis-[Pt(NH 3 ) 2 Cl(OH 2 )] + ion, i.e., the mono-activated form of CP, and cyanocobalamin (also known as vitamin B 12 , see Figure 1) [1,2], may act as one of these prodrugs, that ultimately leads to the release of the Pt(II) complex through intracellular enzymatic conversions [3,4]. This effect is emphasized by the overexpression of CNCbl high-affinity receptors in several tumoral cells.Cellular uptake of cisplatin conjugates is typically followed via detection of their metal constituents by ICP-MS [5,6]. Yet, a mass spectrometric technique providing detailed molecular information, like MALDI-MS, has been recently proposed…”