The impact of vitrification on immature oocyte cell cycle and cytoskeletal integrity in a rat model

Kim, S. Samuel; Olsen, Rachel; Kim, Dojun David; Albertini, David F.

doi:10.1007/s10815-014-0216-1

Cited by 12 publications

(10 citation statements)

References 34 publications

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“…Microfilaments can also be indicators of cryodamage, as reported by [34], where microfilament structure was altered in vitrified human oocytes and did not restore to the physiological configuration before than 3 h after thawing. Precocious cell cycle entry, abnormal chromatin configurations, and disruption of the actin cytoskeleton were observed in vitrified-warmed cumulus-enclosed GV-stage rat oocytes [35].…”

Section: Vitrification and Oocyte Qualitymentioning

confidence: 97%

“…Deleterious effects of vitrification, such as retraction of cumulus cell projections, were observed in vitrified sheep COCs [79], similar to immature vitrified COCs from primed rats, were a reduction of F-actin in ooplasm and oocyte cortex was associated to irreversible but irregular retraction of transzonal projections [35]. The mechanical removal of cumulus cells from immature bovine oocytes did not affect their maturation competence but reduced blastocyst rates, when compared with intact COCs [80].…”

Section: Somatic Compartments In Ivm and Vitrificationmentioning

confidence: 99%

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Vitrification of human immature oocytes before and after in vitro maturation: a review

Khalili

Shahedi

Ashourzadeh

et al. 2017

J Assist Reprod Genet

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The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation may offer some advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation and postponing childbirth. It also eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing. In addition, a successful oocyte cryopreservation program could eliminate the need for donor and recipient menstrual cycle synchronization. Recent advances in vitrification technology have markedly improved the oocyte survival rate after warming, with fertilization and implantation rates comparable with those of fresh oocytes. Healthy live births can be achieved from the combination of IVM and vitrification, even if vitrification of in vivo matured oocytes is still more effective. Recently, attention is given to highlight whether vitrification procedures are more successful when performed before or after IVM, on immature GV-stage oocytes, or on in vitro matured MII-stage oocytes. In this review, we emphasize that, even if there are no differences in survival rates between oocytes vitrified prior to or post-IVM, reduced maturation rates of immature oocytes vitrified prior to IVM can be, at least in part, explained by underlying ultrastructural and biomolecular alterations.

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Section: Vitrification and Oocyte Qualitymentioning

confidence: 97%

Section: Somatic Compartments In Ivm and Vitrificationmentioning

confidence: 99%

Vitrification of human immature oocytes before and after in vitro maturation: a review

Khalili

Shahedi

Ashourzadeh

et al. 2017

J Assist Reprod Genet

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“…al, 1990), this suggested that there was no total benefit to cryopreserving at the germinal vesicle compared with the metaphase II stage. In many studies it has been shown that cryopreserving immature oocytes harms the ooplasm (Kim et. al, 2014), potentially due to inadequate conditions of in vitro maturation or basic oocyte defects.…”

Section: Immature Oocytesmentioning

confidence: 99%

46 Vitrification of Immature and Mature Bovine Oocytes

Hardin

Diaz

Foster

et al. 2017

Reprod. Fertil. Dev.

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While vitrification has become a valuable system used in oocyte and embryo preservation, there is still much to be learned in optimizing this protocol. Both mature and immature oocytes can be vitrified but each presents challenging aspects. Mature oocytes have microfilaments that are not yet developed in immature oocytes, which are fragile and may be disrupted by ice crystal formation during freezing. Further, currently many different cryoprotectants are used in different concentrations, most being combinations of dimethyl sulfoxide (DMSO), glycerol, and ethylene glycol. This study aimed to determine if vitrification solutions composed of ethylene glycol and either dimethyl sulfoxide or glycerol resulted in more-competent post-thaw oocytes, and to determine if maturation stage affected optimal vitrification solution. As validation of the IVF protocol, fresh mature oocytes from a commercial source were fertilized and proportion, with pronuclei formation 48 h post-IVF was recorded. Two experiments evaluated 2 cryoprotectant solutions by analysing post-vitrification and thaw competence of in vitro-fertilized oocytes to form pronuclei. Oocytes in both studies were exposed to 2 sequential vitrification solutions containing 10% DMSO or glycerol, 10% ethylene glycol and 0.5 M sucrose, and then 20% DMSO/glycerol and ethylene glycol and 0.5 M sucrose, before vitrification on cryolocks. In the first study, immature bovine oocytes (n = 200) were vitrified. Following thawing and IVM, they were analysed for pronuclei formation, with 8.49% and 0% fertilization following vitrification in DMSO and glycerol, respectively (P < 0.01). In the second study, mature oocytes were vitrified (n = 200), thawed, and fertilized using the same methods as in study 1. In total, 12.62% and 3.4% of the mature oocytes were successfully fertilized following vitrification in DMSO and glycerol, respectively (P < 0.05). Fisher’s exact test was used for all statistics in both studies. These results suggest that DMSO in combination with ethylene glycol may be superior to glycerol for vitrification of both immature and mature bovine oocytes.

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“…These oocytes resulted in high post thaw survival and, significantly, improved developmental competence [38]. Moreover, a protective effect of antifreeze protein 3 on the cortical F-actin of vitrified/warmed mature mouse oocytes has been reported [44].…”

Section: Discussionmentioning

confidence: 94%

“…The debate on how vitrification procedures affect the microfilament network of the immature or mature oocytes has been reported in several studies [8,13,[17][18][19][20][21][38][39][40]. Consequences of vitrification/warming on the actin cytoskeleton depended on the CPAs used, the oocyte maturation stage and varied between species.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the impact of vitrification on the actin cytoskeleton of in vitro matured ovine oocytes by means of Raman microspectroscopy

Bogliolo

Murrone

Piccinini

et al. 2014

J Assist Reprod Genet

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Purpose Investigation of the changes induced by vitrification on the cortical F-actin of in vitro matured ovine oocytes by Raman microspectroscopy (RMS). Methods Cumulus-oocyte complexes, recovered from the ovaries of slaughtered sheep, were matured in vitro and vitrified following the Minimum Essential Volume method using cryotops. The cortical region of metaphase II (MII) oocytes (1) exposed to vitrification solutions but not cryopreserved (CPA-exp), (2) vitrified/warmed (VITRI), and (3) untreated (CTR) was analyzed by RMS. A chemical map of one quadrant of single CPA-exp, VITRI and CTR oocytes was, also, performed. In order to identify the region of Raman spectra representative of the cortical F-actin modification, a group of in vitro matured oocytes were incubated with latrunculin-A (LATA), a specific F-actin destabilizing drug, and processed for RMS analysis. Thereafter, all the oocytes were stained with rhodamine phalloidin and evaluated by fluorescence confocal microscopy. Raman spectra of the oocytes were, statistically, analyzed using Principal Component Analysis (PCA). Results The PCA score plots showed a marked discrimination between CTR oocytes and CPA-exp/ VITRI groups. The main differences, highlighted by PCA loadings, were referable to proteins (1657, 1440 and 1300 cm ) and, as indicated by LATA experiments, also included the changes of the F-actin. Analysis by confocal microscopy revealed a clear alteration of the cortical F-actin of CPA-exp and VITRI oocytes confirming RMS results. Conclusions Raman microspectroscopy may represent an alternative analytical tool for investigating the biochemical modification of the oocyte cortex, including the F-actin cytoskeleton, during vitrification of in vitro matured ovine oocytes.

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The impact of vitrification on immature oocyte cell cycle and cytoskeletal integrity in a rat model

Cited by 12 publications

References 34 publications

Vitrification of human immature oocytes before and after in vitro maturation: a review

Vitrification of human immature oocytes before and after in vitro maturation: a review

46 Vitrification of Immature and Mature Bovine Oocytes

Evaluation of the impact of vitrification on the actin cytoskeleton of in vitro matured ovine oocytes by means of Raman microspectroscopy

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