The impact of the FOXL2 gene on ovarian granulosa cells KGN

Abstract

Purpose: This study aimed to investigate the impact of JNK regulation on the FOXL2 gene in ovarian granulosa cells KGN. Materials and methods: Bioinformatics methods were employed to identify the main pathogenic gene FOXL2 in ovarian cancer. KGN cells were randomly divided into control and experimental groups, with the experimental group treated with different concentrations of JNK inhibitors (0.1, 1, 5, 10, 50 μM) and the control group receiving an equal volume of DMSO and incubated for 12 hours. The MTT assay was utilized to assess the proliferative capacity of KGN cells treated with JNK inhibitors. A cell scratch test was conducted to evaluate their migration ability. Cell RNA was extracted, reverse transcribed into cDNA, and qRT-PCR was employed to measure the mRNA expression levels of FOXL2. Protein was extracted and Western blot was used to determine the expression levels of FOXL2 protein. Results: JNK inhibitors at concentrations of 1, 5, 10, and 50 μm all led to a decrease in FOXL2 expression. Conclusion: The FOXL2 gene exerts an influence on KGN cells through JNK regulation.