The impact of terahertz radiation on an extremophilic archaean Halorubrum saccharovorum proteome

Горячковская, Н.; Константинова, Г.; Мещерякова, А.; Bannikova, V. P.; Demidov, A.A.; Брянская, В.; Shcheglov, A. V.; Semenov, I. N.; Ощепков, Ю.; Попик, Array М.; Peltek, E.

doi:10.18699/vj16.205

Cited by 2 publications

(2 citation statements)

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“…This observation was confirmed by the rapid and sustained activation of the cellular systems that compensate for the inhibition of the respiratory and glycolytic systems, namely the upregulation of ATP synthase and of a wide range of enzymes that generate NADH. With regards to the translation system, a similar effect was previously shown when cells of the thermostable archaea Halorubrum saccharovorum were exposed to long-term, non-thermal THz radiation (2.3 THz, 5 h), where the expression of genes encoding the structure of translation initiation and elongation factors was also reduced by irradiation [ 63 ].…”

Section: Discussionmentioning

confidence: 60%

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Specific Features of the Proteomic Response of Thermophilic Bacterium Geobacillus icigianus to Terahertz Irradiation

Bannikova

Khlebodarova

Vasilieva

et al. 2022

IJMS

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Studying the effects of terahertz (THz) radiation on the proteome of temperature-sensitive organisms is limited by a number of significant technical difficulties, one of which is maintaining an optimal temperature range to avoid thermal shock as much as possible. In the case of extremophilic species with an increased temperature tolerance, it is easier to isolate the effects of THz radiation directly. We studied the proteomic response to terahertz radiation of the thermophilic Geobacillus icigianus, persisting under wide temperature fluctuations with a 60 °C optimum. The experiments were performed with a terahertz free-electron laser (FEL) from the Siberian Center for Synchrotron and Terahertz Radiation, designed and employed by the Institute of Nuclear Physics of the SB of the RAS. A G. icigianus culture in LB medium was THz-irradiated for 15 min with 0.23 W/cm2 and 130 μm, using a specially designed cuvette. The life cycle of this bacterium proceeds under conditions of wide temperature and osmotic fluctuations, which makes its enzyme systems stress-resistant. The expression of several proteins was shown to change immediately after fifteen minutes of irradiation and after ten minutes of incubation at the end of exposure. The metabolic systems of electron transport, regulation of transcription and translation, cell growth and chemotaxis, synthesis of peptidoglycan, riboflavin, NADH, FAD and pyridoxal phosphate cofactors, Krebs cycle, ATP synthesis, chaperone and protease activity, and DNA repair, including methylated DNA, take part in the fast response to THz radiation. When the response developed after incubation, the systems of the cell’s anti-stress defense, chemotaxis, and, partially, cell growth were restored, but the respiration and energy metabolism, biosynthesis of riboflavin, cofactors, peptidoglycan, and translation system components remained affected and the amino acid metabolism system was involved.

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Section: Discussionmentioning

confidence: 60%

“…Furthermore, it was shown that ATPase HslU, being a molecular chaperon, prevents cell growth inhibitor SulA aggregation and its elimination via HslV-mediated degradation [ 59 , 63 ]. Cell growth deceleration may be a consequence of the increased concentration of HslU during THz irradiation.…”

Section: Discussionmentioning

confidence: 99%