The impact of temperature and Wolbachia infection on vector competence of potential dengue vectors Aedes aegypti and Aedes albopictus in the transmission of dengue virus serotype 1 in southern Taiwan

Tsai, Cheng-Hui; Chen, Tien-Huang; Lin, Cheo; Shu, Pei-Yun; Su, Chien-Ling; Teng, Hwa-Jen

doi:10.1186/s13071-017-2493-x

Cited by 21 publications

(26 citation statements)

References 34 publications

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“…It has also been suggested that temperature has an indirect effect on the vector competence of Aedes aegypti and Ae. albopictus for dengue virus transmissions through temperature dependence of Wolbachia infections (Tsai et al, 2017). To account for this assumed temperature dependency, we considered temperature in the transmission risk model, taking the temperature of the warmest quarter (bio10) provided by worldclim as a separate predictor variable.…”

Section: Resultsmentioning

confidence: 99%

Peer Review #1 of "Vector distribution and transmission risk of the Zika virus in South and Central America (v0.3)"

Ahmadi¹

2019

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Background: Zika is of great medical relevance due to its rapid geographical spread in 2015 and 2016 in South America and its serious implications, e.g. certain birth defects. Recent epidemics urgently require a better understanding of geographic patterns of the Zika virus transmission risk. This study aims to map the Zika virus transmission risk in South and Central America. We applied the maximum entropy approach, which is common for species distribution modelling, but is now also widely in use for estimating the geographical distribution of infection diseases. Methods: As predictor variables we used a set of variables considered to be potential drivers of both direct and indirect effects on the emergence of Zika. Specifically, we considered (a) the modelled habitat suitability for the two main vector species Aedes aegypti and Aedes albopictus as a proxy of vector species distributions; (b) temperature, as it has a great influence on virus transmission; (c) commonly called evidence consensus maps of human Zika virus infections on a regional scale as a proxy for virus distribution; (d) evidence consensus maps of human dengue virus infections and, (e) as possibly relevant socioeconomic factors, population density Vector distribution and transmission risk of the Zika virus in South and Central America

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Section: Resultsmentioning

confidence: 99%

Peer Review #1 of "Vector distribution and transmission risk of the Zika virus in South and Central America (v0.3)"

Ahmadi¹

2019

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“…DENV titers can reach 10 2.67 ± 0.33 to 10 4.09 ± 0.71 PFU equivalents/mL in infected female A. aegypti after oral infection with DENV [36]. The titers are higher than the sensitivity of the pan-DENV RT-iiPCR/POCKIT Central system (Table 1).…”

Section: Detection Of Dengue Virus Serotypes 1-4 In Mosquitosmentioning

confidence: 98%

“…A. aegypti mosquitoes (UGAL strain) were injected individually with DENV-1 (Hawaii Strain, 400 PFU), DENV-2 (NGC Strain, 400 PFU), DENV-3 (DN8700829A Strain, 400 PFU), or DENV-4 (DN9000475A Strain, 400 PFU) by micro-injection (nanoinjector) into the thoracic cavity. Whole mosquitoes were homogenized for plaque forming assay after 0, 1, 3, 5, 7, and 14 days post-infection [36]. DENV was found to be detectable (10 3 -10 4 PFU/ml) in mosquitoes, and viral titers (infectivity) were maintained in the mosquitoes that had been cultured for 2 weeks without significant attenuation.…”

Section: Mosquito Specimensmentioning

confidence: 99%

A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos

Tsai¹,

Liu²,

Lin³

et al. 2019

PLoS ONE

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Background The insulated isothermal PCR (iiPCR) technology enables consistent PCR amplification and detection in a simple heating device. A pan-dengue virus (DENV) RT-iiPCR, targeting the 5’ untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate devices for nucleic acid extraction and PCR amplification/detection) to offer performance comparable to a laboratory real-time PCR. Working on the same technologies, a compact automated sample-in-answer-out system (POCKIT Central Nucleic Acid Analyser) has been available commercially for iiPCR, minimizing human error risks and allowing easy molecular bio-detection near points of need. Here, we evaluated the analytical and clinical performance of the pan-DENV RT-iiPCR on the fully automated system by comparison to those on the semi-automated system. Methodology/Principal findings Testing sera containing serial diluted DENV-1, -2, -3, or -4 cell culture stock, the pan-DENV RT-iiPCR system had similar 100% detection endpoints on the two systems; i.e. at 1, 10, 1 and 10 PFU/ml, respectively, on the fully automated system, and at 10, 1, 10 and 10 PFU/ml, respectively, on the semi-automated system. Furthermore, both fully automated and semi-automated PCR system can detect all four DENV serotypes in mosquitos. Clinical performance of the reagent on the two systems was evaluated by testing 60 human serum samples. Both systems detected the same 40 samples (ten DENV-1, -2, -3, and -4 positive each) and did not detect the other 20; 100% agreement (κ = 1) was found between the two systems. Conclusions/Significance With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease.

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“…Viral titers of dengue are high in the head, thorax (where the salivary glands are located) and midgut of the mosquito (Tsai et al, 2017), which provide reliable dengue positivity screening via molecular methods (Pankhong et al, 2002;Thavara et al, 2006). Thus, in the present study, the head and thorax of individual Aedes mosquitoes were dissected using a forceps and scalpel under a digital microscope (Dino-Lite Edge, AnMo Electronics Corporation, Taiwan) and used for subsequent reverse-transcription Polymerase Chain Reaction (RT-PCR).…”

Section: Rna Extraction and Reverse Transcriptionmentioning

confidence: 99%

Dengue virus surveillance: circulation of DENV-1, DENV-2, DENV-3 and DENV-4 in mosquitoes around Taman Connaught, Cheras

Heng

Chia

Nashwaan

et al. 2019

APJMBB

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Dengue, a mosquito-borne viral infection that causes dengue fever, hemorrhagic fever and dengue shock syndrome, is on a drastic rise in recent years. In Malaysia, the distribution of dengue viruses is determined based on localized studies, where these data are essential to predict the epidemiology of dengue infections. Therefore, a study was conducted in Taman Connaught, Cheras, determining dengue virus serotypes in field caught Aedes mosquitoes. Out of 1,147 mosquitoes captured from September to December 2017, 156 Aedes spp. mosquitoes were identified based on their physical features using a digital microscope. Total RNA was then extracted from the head and thorax of the mosquitoes and subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) with DENV-1, DENV-2, DENV-3 and DENV-4 primer sets, respectively. The results show that at least one DENV serotype was detected in 91% (n=142) out of 156 Aedes spp. mosquitoes. The predominant serotype detected was DENV-2 [47.44% (n=74)], followed by DENV-4 [8.97% (n=14)], DENV-3 [7.05% (n=11)] and DENV-1 [3.85% (n=6)], respectively. Co-circulation of DENV-2 and -4 was the predominant co-infection detected in Aedes spp. mosquitoes. We also present the first report of DENV-1, -2 and -4 co-infection in field-caught Aedes spp. Localized surveillance mechanisms based on serotype circulation in the mosquito vector can be an effective monitoring system for the government and public health sector in order to reduce the incidence of dengue hotspots.

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The impact of temperature and Wolbachia infection on vector competence of potential dengue vectors Aedes aegypti and Aedes albopictus in the transmission of dengue virus serotype 1 in southern Taiwan

Cited by 21 publications

References 34 publications

Peer Review #1 of "Vector distribution and transmission risk of the Zika virus in South and Central America (v0.3)"

Peer Review #1 of "Vector distribution and transmission risk of the Zika virus in South and Central America (v0.3)"

A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos

Dengue virus surveillance: circulation of DENV-1, DENV-2, DENV-3 and DENV-4 in mosquitoes around Taman Connaught, Cheras

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