The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions

Nikolić, Tatjana; Kojić, Danijela; Orčić, Snežana; Batinić, Darko; Vukašinović, Elvira L.; Blagojević, Duško; Purać, Jelena

doi:10.1016/j.chemosphere.2016.08.077

Cited by 59 publications

(39 citation statements)

References 54 publications

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“…Our own research confirms that 12 h exposure to the E-field with a frequency of 50 Hz significantly affects the activity of catalase. Similar fluctuations in catalase activity were observed in the studies of Nikolića et al [ 47 ] who investigated the effect of sublethal concentrations of Cu, Pb, and Cd on catalase activity under laboratory conditions.…”

Section: Discussionsupporting

confidence: 84%

“…Li et al [ 46 ], when examining the effect of pollen nutrition, showed SOD activity above 2.6 U/mg in the middle intestine. Higher results were obtained by Nikolića et al [ 47 ] who analyzed homogenates of whole bees from areas with varying degrees of anthropopressure. Comparing SOD activity in bees from urban, industrial, and control areas, showed that bees from the polluted area have increased response of the antioxidative enzymes.…”

Section: Discussionmentioning

confidence: 84%

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Changes in the Honeybee Antioxidant System after 12 h of Exposure to Electromagnetic Field Frequency of 50 Hz and Variable Intensity

Migdał

Murawska

Strachecka

et al. 2020

Insects

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In recent years, on a global scale, more and more reports of a phenomenon called CCD (Colony Collapse Disorder) have been reported. In addition to pesticides, diseases, and other environmental stressors, electromagnetic fields are also mentioned as one of the possible causes of CCD. One of the body’s first lines of defense against harmful factors is the antioxidant system. We hypothesized that electromagnetic field upregulate the activity of SOD (superoxide dismutase), CAT (catalases), and changed FRAP (total antioxidant potential) in honeybee hemolymph. In our research, 12 h bee’s exposure to E-field was analyzed to determine changes in the antioxidant system. The frequency of 50 Hz and various intensities were used: 5.0 kV/m, 11.5 kV/m, 23.0 kV/m, and 34.5 kV/m. Superoxide dismutase was characterized by four times higher activity in the study groups as compared to the control group. Catalase activity in all groups was characterized by statistically significantly different activity between the groups. The highest activity was recorded in the 34.5 kV/m group. The lowest activity was recorded in the 11.5 kV/m group. A relationship was found between different E-field intensities and changes in the antioxidant system.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 84%

Changes in the Honeybee Antioxidant System after 12 h of Exposure to Electromagnetic Field Frequency of 50 Hz and Variable Intensity

Migdał

Murawska

Strachecka

et al. 2020

Insects

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“…This analysis was part of a larger study showing that exposure to Cd, Cu and Pb at specific concentration (selected based on their presence in pollen and similar bioassays) led to significant changes in redox status, catalase and superoxide dismutase activity and gene expression. The only increase in mortality was observed in the group fed with the highest dose of CuCl 2 (Nikolić et al, ).…”

Section: Discussionmentioning

confidence: 99%

Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure

et al. 2019

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Metallothioneins are ubiquitous proteins important in metal homeostasis and detoxification. However, they have not previously been identified in honey bees or other Hymenoptera, where metallothioneins could be of ecophysiological and ecotoxicological significance. Better understanding of the molecular responses to stress induced by toxic metals could contribute to honey bee conservation. In addition, honey bee metallothionein could represent a biomarker for monitoring environmental quality. Here we identify and characterize a metallothionein gene in Apis mellifera (AmMT). AmMT is 1,680 bp long and encodes a 48 amino acids protein with 15 cysteines and no aromatic residues. A metal response element upstream of the start codon, coupled with numerous cis‐regulatory elements indicate the functional context of AmMT. Molecular modelling predicts several transition metal binding sites, and comparative phylogenetic analysis revealed five putative metallothionein proteins in three other hymenoptera species. AmMT was characterized by cloning the full‐length coding sequence of the putative metallothionein. Recombinant AmMT was found to increase metal tolerance upon overexpression in Escherichia coli supplemented with Cd, Cu or Pb. Finally, in laboratory tests on honey bees, gene expression profiles showed a dose‐dependant relationship between Cd, Cu and Pb concentrations present in food and AmMT expression, while field experiments showed induction of AmMT in bees from an industrial site compared to those from an urban area. These studies suggest that AmMT has metal binding properties in agreement with a possible role in metal homeostasis. Further functional and structural characterization of metallothionein in honey bees and other Hymenoptera are necessary.

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“…Historically, honeybees and hive products were initially used to monitor contamination from heavy metals and radionuclides (Svoboda 1961(Svoboda , 1962, which show high similarity in terms of their chemical properties and dynamics in the biosphere. These contaminants are still monitored by analyzing bees and their products (Badiou-Beneteau et al 2013;Nikolić et al 2016;Gutiérrez et al 2015;Skorbilowicz et al 2018). Later, honeybees were most commonly used to monitor the levels of pesticides in fields and on vegetables visited by honeybees (Quigley et al 2019;Codling et al 2016;Kasiotis et al 2014;Barganska et al 2018;de Oliveira et al 2016).…”

Section: Current Applications Of Biomonitoring Using Honeybeesmentioning

confidence: 99%

Bioindicators and biomonitoring: honeybees and hive products as pollution impact assessment tools for the Mediterranean area

Girotti

Ghini

Ferri

et al. 2020

Euro-Mediterr J Environ Integr

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The global range of the environmental quality issues we all face necessitates integrated action from all of the countries that suffer from a particular environmental issue. We must be able to obtain data on the presence of pollutants and their effects on biota from any territory, regardless of its environmental, infrastructural, social, and economic conditions. Biomonitoring utilizes organisms and natural materials to obtain this information. In particular, the honeybee is a ubiquitous, easy-to-breed organism with great mobility. Its body, which is covered with hairs, picks up materials and particulates that it encounters in the environment. Therefore, bees are highly effective accumulators of materials from the soil, vegetation, air, and water. These characteristics mean that the honeybee is both a bioindicator and a passive bioaccumulator organism, making it an ideal agent for easily monitoring vast areas inexpensively, even in regions where infrastructure is scarce. In this short review, we summarize the main targets of the honeybee-based monitoring campaigns that have been carried out to date, highlighting the results obtained in assessments of organic and inorganic pollution performed by coupling more modern technologies with this long-standing practice. It is hoped that this review will make scientists more aware of the incredible potential of these delicate organisms to provide data that could prove useful in the management of environmental issues.

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The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions

Cited by 59 publications

References 54 publications

Changes in the Honeybee Antioxidant System after 12 h of Exposure to Electromagnetic Field Frequency of 50 Hz and Variable Intensity

Changes in the Honeybee Antioxidant System after 12 h of Exposure to Electromagnetic Field Frequency of 50 Hz and Variable Intensity

Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure

Bioindicators and biomonitoring: honeybees and hive products as pollution impact assessment tools for the Mediterranean area

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