The impact of sperm DNA fragmentation on ICSI outcome in cases of donated oocytes

Antonouli, Sevastiani; Papatheodorou, Achilleas; Panagiotidis, Yannis; Petousis, Stamatios; Prapas, Nikos; Nottola, Stefania Annarita; Palmerini, Maria Grazia; Macchiarelli, Guido; Prapas, Yannis

doi:10.1007/s00404-019-05133-9

Cited by 35 publications

(35 citation statements)

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“…Female BMI in high sperm DFI group (15-30%) was significantly lower compared with that in low sperm DFI group (< 15%), while the distribution of primary infertility and thickness of the endometrium in the 15-30% group were significantly higher compared with that in the DFI < 15% group. Female age and male age were significantly different among the three groups, while sperm DFI significantly increased as female age and male age increased (Table 1), which was consistent with the reported literatures [12,14].…”

Section: Resultssupporting

confidence: 91%

“…Another two meta-analyses concluded that sperm DFI did not predict IVF/ICSI outcomes [10,11]. Antonouli et al [12] reported that sperm DFI did not significantly affect embryonic development or pregnancy rate in ICSI patients with donated oocytes. From the literatures published above, the effect of sperm DFI on embryonic development, implantation and pregnancy remained controversial after IVF/ICSI.…”

Section: Introductionmentioning

confidence: 99%

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Effects of the sperm DNA fragmentation index on the clinical and neonatal outcomes of intracytoplasmic sperm injection cycles

et al. 2020

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Background: Most studies have mainly focused on the effects of the sperm DNA fragmentation index (DFI) on fertilization, embryonic developmental potential and aneuploidy, pregnancy and abortion rates after in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) and have remained controversial. However, few studies have reported the effects of sperm DFI on neonatal outcomes, including stillbirths, neonatal deaths, sex, gestational age, prematurity, birthweight, low birth weight (LBW) and birth defects in newborns. Our objective was to evaluate the effects of sperm DFI on the clinical and neonatal outcomes of ICSI cycles. Methods: This retrospective study analysed a total of 2067 oocyte retrieval, 1139 transfer and 713 delivery cycles from conventional ICSI cycles, including 301, 469, and 214 live-born infants in groups segregated according to sperm DFI as the < 15%, 15-30% and > 30% groups, respectively. The clinical and neonatal outcomes were compared among the three groups. Results: Sperm DFI did not significantly affect the rates of fertilization, clinical pregnancy, miscarriage or ongoing pregnancy. Sperm DFI did not increase the risk of stillbirths or neonatal deaths. The rates of stillbirths and neonatal deaths were not significantly different among the three groups. The sex, gestational age, prematurity, birthweight and LBW of newborns in the three groups were not significantly affected by sperm DFI. Moreover, sperm DFI did not increase the number of birth defects in children. Conclusions: Sperm DFI did not affect the clinical or neonatal outcomes of ICSI cycles.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Effects of the sperm DNA fragmentation index on the clinical and neonatal outcomes of intracytoplasmic sperm injection cycles

et al. 2020

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“…Several studies suggest that sperm DNA damage is more frequent in infertile men compared with fertile men. [39][40][41][42][43] Patients presenting with oligozoospermia have more sperm chromatin alterations that reduce embryo development. 44 A relationship between the level of sperm DNA fragmentation and impaired embryo development after ICSI has been reported in several studies.…”

Section: Discussionmentioning

confidence: 99%

Increasing paternal age and ejaculatory abstinence length negatively influence the intracytoplasmic sperm injection outcomes from egg‐sharing donation cycles

et al. 2019

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Background:The paternal role in embryogenesis is much more than providing a haploid genome. The spermatozoa have crucial roles related to embryogenesis' epigenetic regulation, fusion of gametes, and cleavage, which may influence intracytoplasmic sperm injection outcomes. Objectives:To study whether paternal age, length of ejaculatory abstinence and semen parameters influence the outcomes of intracytoplasmic sperm injection in the oocyte recipients from egg-sharing donation cycles. Materials and methods:This historical cohort study involved 427 oocyte recipient intracytoplasmic sperm injection cycles, from 321 patients undergoing egg-sharing donation cycles from January 2015 to May 2017, in a private in vitro fertilization center.Electronic medical records of donors and recipients intracytoplasmic sperm injection cycles were reviewed. The impact of male age, length of ejaculatory abstinence, and semen parameters on recipients' intracytoplasmic sperm injection outcomes were investigated using general mixed models. Implantation, pregnancy, and live birth rates were the primary outcome measures. Results: Paternal age negatively affected fertilization, day 3 top-quality embryos, day 3 normal embryo cleavage speed, blastocyst formation, high-quality blastocysts, and implantation, and was correlated with diminished pregnancy and live birth rates. Length of ejaculatory abstinence negatively influenced the rates of day 3 top-quality embryos, normal embryo development on day 3, blastocyst development, and implantation. Sperm count positively affected the rates of fertilization, blastocyst development, and implantation. The percentage of progressive sperm motility positively affected day 3 normal embryo development rate and implantation rate. Total motile sperm count positively affected blastocyst development rate and implantation rate. Discussion and Conclusion: Additional tracking of the influence of male characteristics on intracytoplasmic sperm injection outcomes should be stimulated. Paternal age is not manageable, but reducing length of ejaculatory abstinence could be an alternative approach to improve the outcomes of intracytoplasmic sperm injection. | 595 SETTI ET al. How to cite this article: Setti AS, Braga DPAF, Iaconelli Junior A, Borges Junior E. Increasing paternal age and ejaculatory abstinence length negatively influence the intracytoplasmic sperm injection outcomes from egg-sharing donation cycles.

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“… 31 Besides, although SCD tests measured fragmentation indirectly that was less sensitive than others, it might reflect later outcomes such as birth delivery and abortion rates. 14 , 42 Primarily, because of the advantages of the SCD assay, we used it along with the equipment available in our laboratory to assess sperm DNA fragmentation levels during this study. Moreover, previous studies have shown that SCD can help predict the prognosis for male infertility and provide results similar to those of other methods when assessing sperm DNA fragmentation levels.…”

Section: Discussionmentioning

confidence: 99%

“… 12 Conversely, other studies concluded that the DFI was not correlated with blastocyst aneuploidy or morphological grading, 13 total blastocyst number, or the number of blastocysts with good quality. 14 …”

Section: Introductionmentioning

confidence: 99%

Predictive Significance of Sperm DNA Fragmentation Testing in Early Pregnancy Loss in Infertile Couples Undergoing Intracytoplasmic Sperm Injection

Lê

Nguyen

et al. 2021

RRU

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Objective This study aimed to determine the role of sperm DNA fragmentation as a marker that could predict early pregnancy loss (EPL), either independently or in combination with another marker or markers, after intracytoplasmic sperm injection (ICSI) cycles. Methods This prospective descriptive cohort study retrieved data from 162 couples who underwent their first ICSI cycles at the Center for Reproductive Endocrinology and Infertility of Hue University Hospital in Vietnam from May 2018 to December 2019. General characteristics, semen parameters, sperm DNA fragmentation index (DFI), fertilization, embryo development, pregnancy rates, and EPL were assessed. The receiver-operating characteristic (ROC) method was performed to identify the threshold of DFI in EPL. Multivariate analysis was used to demonstrate the relationship between the sperm DNA fragmentation level and EPL. Results Of 162 ICSI cycles, 23 (14.2%) involved EPL. There was no significant difference between the sperm DNA fragmentation rate and the overall rate of pregnancy loss, although the negative pregnancy outcome group had a median DFI that was higher than that of the positive pregnancy outcome group (20% vs 17.8%). The ROC analysis indicated that a sperm DNA fragmentation rate of 16.6% was the priority cut-off that could be used to distinguish EPL with a sensitivity of 73.9% (95% confidence interval [CI], 67.15–80.67) and specificity of 47.48% (95% CI, 39.79–55.17). The multivariate analysis confirmed that in female factors such as age, body mass index (BMI), and sperm DNA fragmentation level affected the EPL rate. However, a combination of the sperm DNA fragmentation level and female age or female BMI could not sufficiently predict EPL. Conclusion EPL can be affected by multiple factors including sperm DNA fragmentation; however, there is no sufficient evidence indicating that sperm DNA fragmentation, both as a single marker and combined with other markers, is a good predictor of EPL.

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The impact of sperm DNA fragmentation on ICSI outcome in cases of donated oocytes

Cited by 35 publications

References 58 publications

Effects of the sperm DNA fragmentation index on the clinical and neonatal outcomes of intracytoplasmic sperm injection cycles

Effects of the sperm DNA fragmentation index on the clinical and neonatal outcomes of intracytoplasmic sperm injection cycles

Increasing paternal age and ejaculatory abstinence length negatively influence the intracytoplasmic sperm injection outcomes from egg‐sharing donation cycles

Predictive Significance of Sperm DNA Fragmentation Testing in Early Pregnancy Loss in Infertile Couples Undergoing Intracytoplasmic Sperm Injection

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