The impact of quorum sensing on the virulence of<i>Vibrio anguillarum</i>towards gnotobiotic sea bass (<i>Dicentrarchus labrax</i>) larvae

“…Besides its therapeutic benefits, Z. acanthopodium is expected to be used in more widely application such as in the aquaculture industry. One of the common problems in aquaculture is a pathogenic infection caused by Tenacibaculum maritimum, Vibrio alginolyticus, V. anguillarum, V. harveyi (Li et al 2018;Liu et al 2016; B I OD I V E R S I TA S 21 (9): 4147-4154, September 2020 4148 Småge et al 2016). The administration of antibiotics in aquaculture to cure or prevent infection has become a serious problem since it leads to antimicrobial resistance in pathogens (Mo et al 2015;Santos and Ramos 2018).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit

et al. 2020

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Abstract. Sibero MT, Siswanto AP, Frederick EH, Wijaya AP, Syafitri E, Farabi K, Murwani R, Saito S, Igaras Y. 2020. Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit. Biodiversitas 21: 4147-4154. The local community in North Sumatra has utilized andaliman fruit (Zanthoxylum acanthopodium) as spices for traditional cuisines because it has a unique flavor. Information on the antimicrobial activity of Z. acanthopodium fruit against aquaculture pathogens and its bioactivity against leukemia cell lines are limited. The purposes of this study were to evaluate the antimicrobial activity of Z. acanthopodium fruit against Tenacibaculum maritimum, Vibrio alginolyticus, V. anguillarum, V. harveyi that are known as pathogens in aquaculture; to determine cytotoxic property against murine P388 leukemia cells; and to characterize its metabolites profile. The sample was extracted using methanol by the maceration method. Antibacterial assay was conducted by Kirby-Bauer disc diffusion method; while cytotoxicity assay using the XTT method. Proximate analysis showed that Z. acanthopodium fruit contained 63.41% of moisture, 24.73% of crude fiber, 9.81% of crude protein, 6.90% of ash, and 2.55% of crude fat. Several phytochemical components were detected, such as alkaloid, flavonoid, tannin, triterpenoid, and steroid. The GC/MS analysis indicated the presence of various compounds from terpenoid and terpenes derivatives. This study indicated that Z. acanthopodium fruit was not potential as antibacterial agents against the aquaculture pathogens; however, the methanol extract showed cytotoxic potential with IC50 19.14 µg/mL against murine P388 leukemia cells.

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“…aeruginosa . V. anguillarum is a Gram-negative bacterium and causative agent of vibriosis, which is pathogenic to a variety of aquatic organisms, such as fish and crustaceans, with importance in aquaculture industry [76,77,78,79]. Ps.…”

Section: Discussionmentioning

confidence: 99%

Rapid Metabolome and Bioactivity Profiling of Fungi Associated with the Leaf and Rhizosphere of the Baltic Seagrass Zostera marina

et al. 2019

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Zostera marina (eelgrass) is a marine foundation species with key ecological roles in coastal habitats. Its bacterial microbiota has been well studied, but very little is known about its mycobiome. In this study, we have isolated and identified 13 fungal strains, dominated by Penicillium species (10 strains), from the leaf and the root rhizosphere of Baltic Z. marina. The organic extracts of the fungi that were cultured by an OSMAC (One-Strain–Many-Compounds) regime using five liquid culture media under both static and shaking conditions were investigated for their chemical and bioactivity profiles. All extracts showed strong anti-quorum sensing activity, and the majority of the Penicillium extracts displayed antimicrobial or anti-biofilm activity against Gram-negative environmental marine and human pathogens. HPLC-DAD-MS-based rapid metabolome analyses of the extracts indicated the high influence of culture conditions on the secondary metabolite (SM) profiles. Among 69 compounds detected in all Penicillium sp. extracts, 46 were successfully dereplicated. Analysis of SM relatedness in culture conditions by Hierarchical Cluster Analysis (HCA) revealed generally low similarity and showed a strong effect of medium selection on chemical profiles of Penicillium sp. This is the first study assessing both the metabolite and bioactivity profile of the fungi associated with Baltic eelgrass Z. marina.

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The impact of quorum sensing on the virulence ofVibrio anguillarumtowards gnotobiotic sea bass (Dicentrarchus labrax) larvae

Cited by 6 publications

References 29 publications

The impact of the multichannel quorum sensing systems of Vibrio tasmaniensis and Vibrio crassostreae on virulence towards blue mussel (Mytilus edulis) larvae

The impact of the multichannel quorum sensing systems of Vibrio tasmaniensis and Vibrio crassostreae on virulence towards blue mussel (Mytilus edulis) larvae

Antibacterial, cytotoxicity and metabolite profiling of crude methanolic extract from andaliman (Zanthoxylum acanthopodium) fruit

Rapid Metabolome and Bioactivity Profiling of Fungi Associated with the Leaf and Rhizosphere of the Baltic Seagrass Zostera marina

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