The impact of PLGA scaffold orientation on in vitro cartilage regeneration

Zhang, Yingying; Yang, Fei; Li, Kai; Shen, Hong; Zhu, Yonglin; Zhang, Wenjie; Liu, Wei; Wang, Shenguo; Cao, Yilin; Zhou, Guangdong

doi:10.1016/j.biomaterials.2012.01.006

Cited by 111 publications

(106 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, our results are not in agreement with what the traditionally thought of in terms of having the aligned nanofi bers to be better suited than random nanofi bers for producing cartilage tissue in vitro. [ 13,19,39 ] Regarding the role of nanofi bers dimensionality, our PCR data unexpectedly show a signifi cantly higher cartilage specifi c gene activity in 2D environment versus the 3D environment, in agreement with the visual morphological inspection above for favored Collagen II and glycosaminoglycans deposition. We eliminated possible differences in medium or nutrition exposure of the two environments by measuring the activity of only middle part of construct.…”

Section: Earlysupporting

confidence: 85%

“…Nevertheless, our nanofi bers scaffolds strongly supersede the pellets in their ability to enhance chondrogenic differentiation of ADSCs thus confi rming other studies indicating increased ECM production and cartilage specifi c gene expression on polymer fi brous scaffolds, while supporting cell proliferation. [ 13,43 ] Typically, organized fi brils within a scaffold are thought to promote cell migration and thus probably contribute to improving cartilage regeneration. [ 13,39,44 ] Our previous studies with endothelial cells also support this view [ 27 ] contrary to typical observations.…”

Section: Earlymentioning

confidence: 99%

“…However the data for other cell types including chondrocytes are rather sparse and controversial. [13,14] Recent findings demonstrated that oriented nanofibrous scaffolds have the potential for engineering blood vessels, [12] neural tissue, [15] and ligaments, [16] for cartilage, however, such studies are rather missing or incomplete. [17][18][19] Another important issue is the source of the cells that might promise proper cartilage repair.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen‐Based Nanofibers

Forget¹,

Awaja

Gugutkov

et al. 2016

Macromolecular Bioscience

View full text Add to dashboard Cite

Mimicking the complex intricacies of the extra cellular matrix including 3D configurations and aligned fibrous structures were traditionally perused for producing cartilage tissue from stem cells. This study shows that human adipose derived mesenchymal stem cells (hADMSCs) establishes significant chondrogenic differentiation and may generate quality cartilage when cultured on 2D and randomly oriented fibrinogen/poly‐lactic acid nanofibers compared to 3D sandwich‐like environments. The adhering cells show well‐developed focal adhesion complexes and actin cytoskeleton arrangements confirming the proper cellular interaction with either random or aligned nanofibers. However, quantitative reverse transcription‐polymerase chain reaction analysis for Collagen 2 and Collagen 10 genes expression confirms favorable chondrogenic response of hADMSCs on random nanofibers and shows substantially higher efficacy of their differentiation in 2D configuration versus 3D constructs. These findings introduce a new direction for cartilage tissue engineering through providing a simple platform for the routine generation of transplantable stem cells derived articular cartilage replacement that might improve joint function.

show abstract

Section: Earlysupporting

confidence: 85%

Section: Earlymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen‐Based Nanofibers

Forget¹,

Awaja

Gugutkov

et al. 2016

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…A porous structure manufactured with a processing technique that can add to this versatility is required; this can be achieved with electrospinning. Electrospinning is an ideal technique for producing 3D networks of fibers of tunable size, orientation, composition, and density that mimic the properties of native extracellular matrix (ECM)1, 2, 3 and can generate scaffolds with spatially arranged functionalization through layering of various polymers during electrospinning 4, 5, 6, 7, 8, 9. Developing a controlled method that allows multiple zonally arranged functional groups within a continuous scaffold allows for the production of a hierarchical structure that can modulate cell behavior within each functional zone.…”

Section: Introductionmentioning

confidence: 99%

Modular and Versatile Spatial Functionalization of Tissue Engineering Scaffolds through Fiber‐Initiated Controlled Radical Polymerization

Harrison

Steele

Chapman

et al. 2015

Adv Funct Materials

View full text Add to dashboard Cite

Native tissues are typically heterogeneous and hierarchically organized, and generating scaffolds that can mimic these properties is critical for tissue engineering applications. By uniquely combining controlled radical polymerization (CRP), end‐functionalization of polymers, and advanced electrospinning techniques, a modular and versatile approach is introduced to generate scaffolds with spatially organized functionality. Poly‐ε‐caprolactone is end functionalized with either a polymerization‐initiating group or a cell‐binding peptide motif cyclic Arg‐Gly‐Asp‐Ser (cRGDS), and are each sequentially electrospun to produce zonally discrete bilayers within a continuous fiber scaffold. The polymerization‐initiating group is then used to graft an antifouling polymer bottlebrush based on poly(ethylene glycol) from the fiber surface using CRP exclusively within one bilayer of the scaffold. The ability to include additional multifunctionality during CRP is showcased by integrating a biotinylated monomer unit into the polymerization step allowing postmodification of the scaffold with streptavidin‐coupled moieties. These combined processing techniques result in an effective bilayered and dual‐functionality scaffold with a cell‐adhesive surface and an opposing antifouling non‐cell‐adhesive surface in zonally specific regions across the thickness of the scaffold, demonstrated through fluorescent labelling and cell adhesion studies. This modular and versatile approach combines strategies to produce scaffolds with tailorable properties for many applications in tissue engineering and regenerative medicine.

show abstract

“…That is why polyesters have been studied for a long time [14][15][16][17][18][19][20]. A lot of efforts have been done on improving the biocompatibility of the polyester porous scaffolds [21][22][23][24][25][26][27][28], and examinations of their medical applications [27][28][29][30][31][32][33].…”

mentioning

confidence: 99%

Effects of L-lactic acid and D,L-lactic acid on viability and osteogenic differentiation of mesenchymal stem cells

Wang

Ding

2013

Chin. Sci. Bull.

View full text Add to dashboard Cite

Poly(lactic acid) (PLA) and other aliphatic polyesters containing the unit of lactic acid are very popular biodegradable materials. While the degradation products, lactic acids, have been worried to bring with negative influence on biocompatibility, the focused experimental studies are less reported. This study is aimed at an in vitro examination of cytotoxicity of both L-lactic acid and D,L-lactic acid. Mesenchymal stem cells (MSCs) derived from rat bone marrow are employed to test the cytotoxicity of the lactic acids. Considering that the addition of lactic acids not only introduces lactate groups but also alters medium pH and ion strength, these three candidate effects are examined in a decoupled way by setting different comparison groups. The results confirm that the change of medium pH is the predominant factor. It has also been found that D-lactate is more cytotoxic than L-lactate at high concentrations. Yet, either L-or D,L-lactic acids seem acceptable in most of medical applications, because the cytotoxicity is significant only when the concentrations are as high as 20 mmol/L for both of them.

show abstract

The impact of PLGA scaffold orientation on in vitro cartilage regeneration

Cited by 111 publications

References 46 publications

Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen‐Based Nanofibers

Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen‐Based Nanofibers

Modular and Versatile Spatial Functionalization of Tissue Engineering Scaffolds through Fiber‐Initiated Controlled Radical Polymerization

Effects of L-lactic acid and D,L-lactic acid on viability and osteogenic differentiation of mesenchymal stem cells

Contact Info

Product

Resources

About