The impact of photobiomodulation on the chondrogenic potential of adipose-derived stromal/stem cells

Schneider, C.; Dungel, Peter; Priglinger, Eleni; Danzer, Martin; Schädl, Barbara; Nürnberger, Sylvia

doi:10.1016/j.jphotobiol.2021.112243

Cited by 14 publications

(7 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Photobiomodulation was reported to induce stem cell migration and proliferation promoting tissue regeneration (33). It was also demonstrated enhance the chondrogenic potential of ADSCs (34). In a similar study, combining ADSCs with photobiomodulation in knee arthritic models signi cantly ameliorated tissue regeneration through reduction of proin ammatory regulators and upregulation of tissue inhibitors of metalloproteinase (TIMP) and collagen 2 (COL 2) expression in the laser associated groups more than the groups treated with ADSCs only or laser only (35).…”

Section: Discussionmentioning

confidence: 95%

Photobiostimulation conjugated with stem cells or their secretome for treatment of temporomandibular joint arthritis; a comparative study

El-Qashty,

Elkashty,

Hany

2023

Preprint

View full text Add to dashboard Cite

Background: Temporomandibular joint (TMJ) arthritis is a debilitating, challenging condition and different methods have been implicated for its treatment. This study aimed to test the therapeutic potentials of low-level laser therapy (LLLT) associated with adipose derived stem cells (ADSC) or their derived secretome on a murine model induced arthritis. Methods: Forty eight rats were divided into four groups where group I was the sham control, the rest of animals were subjected to arthritis induction using complete Freund’s adjuvant, then divided as follows: group II received phosphate buffered saline (PBS) intraarticular injection and irradiation of 0 j/cm2, group III received ADSCs derived secretome and irradiation of 38 j/cm2, and group IV received ADSCs and irradiation of 38 j/cm2 as well. One and three weeks after treatment, animals were euthanized and histological, histomorphometrical, histochemical, and immunohistochemical analysis were performed with the relevant statistical tests. Results: The arthritis group showed histological signs of joint injury including cartilage atrophy, articular disc fibrosis, irregular osteochondral interface, and condylar bone resorption together with high inflammatory reaction and defective proteoglycan content. In contrast, the treated groups III and IV showed much restoration of the joint structure with normal cartilage and disc thickness. The inflammation process was significantly suppressed especially after three weeks, and the cartilage proteoglycan content was much enhanced. No significant difference between the results of the two treated groups was detected. Conclusion: LLLT conjugated with ADSCs or ADSCs derived secretome can efficiently enhance the healing of arthritic TMJs. Stem cell secretome can be applied as a safe, potent therapy. However, further investigations are required to unravel its mechanism of action and pave its way as a safe, novel, cell free therapy.

show abstract

Section: Discussionmentioning

confidence: 95%

Photobiostimulation conjugated with stem cells or their secretome for treatment of temporomandibular joint arthritis; a comparative study

El-Qashty,

Elkashty,

Hany

2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Various physical and chemical factors can be used to induce differentiation of the MSCs into the hyaline cartilage's chondrocytes. The main physical factors affecting the proliferation of chondrogenic cells are periodic mechanical stress, like cyclic strain or fluid shear stress [84][85][86], hypoxia [87], and electromagnetic radiation, so-called photobiomodulation [88][89][90][91]. Subsequent transformation of the physical signal into a biochemical signal is mediated by integrins and focal adhesion [92].…”

Section: The Clinically Used Direct Methods Of the Msc Differentiatio...mentioning

confidence: 99%

Bioadhesive and Injectable Hydrogels and Their Correlation with Mesenchymal Stem Cells Differentiation for Cartilage Repair: A Mini-Review

Kováč,

Priščáková,

Gbelcová

et al. 2023

Polymers

View full text Add to dashboard Cite

Injectable bioadhesive hydrogels, known for their capacity to carry substances and adaptability in processing, offer great potential across various biomedical applications. They are especially promising in minimally invasive stem cell-based therapies for treating cartilage damage. This approach harnesses readily available mesenchymal stem cells (MSCs) to differentiate into chondrocytes for cartilage regeneration. In this review, we investigate the relationship between bioadhesion and MSC differentiation. We summarize the fundamental principles of bioadhesion and discuss recent trends in bioadhesive hydrogels. Furthermore, we highlight their specific applications in conjunction with stem cells, particularly in the context of cartilage repair. The review also encompasses a discussion on testing methods for bioadhesive hydrogels and direct techniques for differentiating MSCs into hyaline cartilage chondrocytes. These approaches are explored within both clinical and laboratory settings, including the use of genetic tools. While this review offers valuable insights into the interconnected aspects of these topics, it underscores the need for further research to fully grasp the complexities of their relationship.

show abstract

“…Instead, systemic signals may originate from biochemical stimuli (cyclic dietary intake that changes the metabolic activity, e.g., the availability of glucose), biomechanical stimuli (locomotion activities such as dynamic compressive mechanical load), or temperature stimuli (diurnal temperature changes in the body) [ 14 , 34 ]. However, the chondrogenic differentiation of certain stem cells can be influenced by the application of photobiomodulation [ 35 ], which suggests that if some of the peripheral joints (such as joints of the hand) are not completely dark, the tissues and cells of the synovial joint, including articular cartilage, may react to specific wavelengths of light.…”

Section: The Molecular Clock In Cartilage Developmentmentioning

confidence: 99%

Hypoxic Conditions Modulate Chondrogenesis through the Circadian Clock: The Role of Hypoxia-Inducible Factor-1α

Juhász,

Hajdú,

Kovács

et al. 2024

Cells

View full text Add to dashboard Cite

Hypoxia-inducible factor-1 (HIF-1) is a heterodimer transcription factor composed of an alpha and a beta subunit. HIF-1α is a master regulator of cellular response to hypoxia by activating the transcription of genes that facilitate metabolic adaptation to hypoxia. Since chondrocytes in mature articular cartilage reside in a hypoxic environment, HIF-1α plays an important role in chondrogenesis and in the physiological lifecycle of articular cartilage. Accumulating evidence suggests interactions between the HIF pathways and the circadian clock. The circadian clock is an emerging regulator in both developing and mature chondrocytes. However, how circadian rhythm is established during the early steps of cartilage formation and through what signaling pathways it promotes the healthy chondrocyte phenotype is still not entirely known. This narrative review aims to deliver a concise analysis of the existing understanding of the dynamic interplay between HIF-1α and the molecular clock in chondrocytes, in states of both health and disease, while also incorporating creative interpretations. We explore diverse hypotheses regarding the intricate interactions among these pathways and propose relevant therapeutic strategies for cartilage disorders such as osteoarthritis.

show abstract

The impact of photobiomodulation on the chondrogenic potential of adipose-derived stromal/stem cells

Cited by 14 publications

References 60 publications

Photobiostimulation conjugated with stem cells or their secretome for treatment of temporomandibular joint arthritis; a comparative study

Photobiostimulation conjugated with stem cells or their secretome for treatment of temporomandibular joint arthritis; a comparative study

Bioadhesive and Injectable Hydrogels and Their Correlation with Mesenchymal Stem Cells Differentiation for Cartilage Repair: A Mini-Review

Hypoxic Conditions Modulate Chondrogenesis through the Circadian Clock: The Role of Hypoxia-Inducible Factor-1α

Contact Info

Product

Resources

About