The impact of Ni, Co and Mo supplementation on methane yield from anaerobic mono-digestion of maize silage

Evranos, Beyza; Demirel, Burak

doi:10.1080/09593330.2014.997297

Cited by 46 publications

(22 citation statements)

References 38 publications

(45 reference statements)

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“…To avoid a possible undersupply of TE, commercial TE supplements are added to biogas reactors based on the operator’s experience (Lemmer et al, 2010; Schmidt, 2011; Lindorfer et al, 2012; Schmidt et al, 2013; Evranos and Demirel, 2015). Correct dosing of TE supplements is very important, since undersupply can cause process instability or low methane yield, whereas overdosage may have toxic effects on the microorganisms and impairs the compliance of the digestate with the requirements for fertilizer (Thanh et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Trace Elements Induce Predominance among Methanogenic Activity in Anaerobic Digestion

et al. 2016

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Trace elements (TE) play an essential role in all organisms due to their functions in enzyme complexes. In anaerobic digesters, control, and supplementation of TEs lead to stable and more efficient methane production processes while TE deficits cause process imbalances. However, the underlying metabolic mechanisms and the adaptation of the affected microbial communities to such deficits are not yet fully understood. Here, we investigated the microbial community dynamics and resulting process changes induced by TE deprivation. Two identical lab-scale continuous stirred tank reactors fed with distiller’s grains and supplemented with TEs (cobalt, molybdenum, nickel, tungsten) and a commercial iron additive were operated in parallel. After 72 weeks of identical operation, the feeding regime of one reactor was changed by omitting TE supplements and reducing the amount of iron additive. Both reactors were operated for further 21 weeks. Various process parameters (biogas production and composition, total solids and volatile solids, TE concentration, volatile fatty acids, total ammonium nitrogen, total organic acids/alkalinity ratio, and pH) and the composition and activity of the microbial communities were monitored over the total experimental time. While the methane yield remained stable, the concentrations of hydrogen sulfide, total ammonia nitrogen, and acetate increased in the TE-depleted reactor compared to the well-supplied control reactor. Methanosarcina and Methanoculleus dominated the methanogenic communities in both reactors. However, the activity ratio of these two genera was shown to depend on TE supplementation explainable by different TE requirements of their energy conservation systems. Methanosarcina dominated the well-supplied anaerobic digester, pointing to acetoclastic methanogenesis as the dominant methanogenic pathway. Under TE deprivation, Methanoculleus and thus hydrogenotrophic methanogenesis was favored although Methanosarcina was not overgrown by Methanoculleus. Multivariate statistics revealed that the decline of nickel, cobalt, molybdenum, tungsten, and manganese most strongly influenced the balance of mcrA transcripts from both genera. Hydrogenotrophic methanogens seem to be favored under nickel- and cobalt-deficient conditions as their metabolism requires less nickel-dependent enzymes and corrinoid cofactors than the acetoclastic and methylotrophic pathways. Thus, TE supply is critical to sustain the activity of the versatile high-performance methanogen Methanosarcina.

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Section: Introductionmentioning

confidence: 99%

Trace Elements Induce Predominance among Methanogenic Activity in Anaerobic Digestion

et al. 2016

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“…Ni and Co are important metal ions for methanogenesis and their supplementation to anaerobic bioreactors may be beneficial for methane production (Gustavsson et al, 2011; Evranos and Demirel, 2015). However, several studies show that no significant enhancement is obtained from metal addition, or that the benefit is dependent on the substrate fed to the microorganisms (Zandvoort et al, 2006b; Park et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effect of Nickel and Cobalt on Methanogenic Enrichment Cultures and Role of Biogenic Sulfide in Metal Toxicity Attenuation

Paulo

Ramiro-García

Mourik³

et al. 2017

Front. Microbiol.

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Metals play an important role in microbial metabolism by acting as cofactors for many enzymes. Supplementation of biological processes with metals may result in improved performance, but high metal concentrations are often toxic to microorganisms. In this work, methanogenic enrichment cultures growing on H2/CO2 or acetate were supplemented with trace concentrations of nickel (Ni) and cobalt (Co), but no significant increase in methane production was observed in most of the tested conditions. However, high concentrations of these metals were detrimental to methanogenic activity of the cultures. Cumulative methane production (after 6 days of incubation) from H2/CO2 was 40% lower in the presence of 8 mM of Ni or 30 mM of Co, compared to controls without metal supplementation. When acetate was used as substrate, cumulative methane production was also reduced: by 18% with 8 mM of Ni and by 53% with 30 mM of Co (after 6 days of incubation). Metal precipitation with sulfide was further tested as a possible method to alleviate metal toxicity. Anaerobic sludge was incubated with Co (30 mM) and Ni (8 mM) in the presence of sulfate or sulfide. The addition of sulfide helped to mitigate the toxic effect of the metals. Methane production from H2/CO2 was negatively affected in the presence of sulfate, possibly due to competition of hydrogenotrophic methanogens by sulfate-reducing bacteria. However, in the enrichment cultures growing on acetate, biogenically produced sulfide had a positive effect and more methane was produced in these incubations than in similar assays without sulfate addition. The outcome of competition between methanogens and sulfate-reducing bacteria is a determinant factor for the success of using biogenic sulfide as detoxification method.

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“…Nanotechnology and NPs are attracting increasing attention among researchers owing to their unique characteristics. NPs are used in various branches of science and industry, also to improve the productivity of biogas . However, further studies are needed to understand fully the impact of NPs on activity of microorganisms, and to determine optimal conditions for an AD process.…”

Section: Feedstocks Technologies and Factors Design To Intensify Bimentioning

confidence: 99%

Intensification of biogas production using various technologies: A review

et al. 2020

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Summary Anaerobic digestion (AD) is an organic matter conversion technology which offers a wide range of options for production of biogas from organic biomass, providing an excellent opportunity to convert abundant bioresources into safe, eco‐friendly, renewable energy. Important factors in the process of AD are the biodiversity of microorganisms, chemical load of oxygen demand, and content of water and total solids. A challenge for the future is to find technologies that will maximally enhance biogas production and to find pathways for biogas to supersede well‐established technologies and practices in the contemporary heavily fossil fuel‐based energy system. Current studies on technologies of biogas production indicate a number of possibilities of using appropriate biological and physicochemical additives, like added enzymes or fruit pomace, as well as immobilizing microorganisms on biofilters. Anaerobic biorefinery is an emerging concept that generates not only bioenergy but also high‐value biochemical products from the same feedstock. This study is a review of articles describing the intensification of biogas production by using various technologies.

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The impact of Ni, Co and Mo supplementation on methane yield from anaerobic mono-digestion of maize silage

Cited by 46 publications

References 38 publications

Trace Elements Induce Predominance among Methanogenic Activity in Anaerobic Digestion

Trace Elements Induce Predominance among Methanogenic Activity in Anaerobic Digestion

Effect of Nickel and Cobalt on Methanogenic Enrichment Cultures and Role of Biogenic Sulfide in Metal Toxicity Attenuation

Intensification of biogas production using various technologies: A review

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