The impact of nanoparticles on the mucosal translocation and transport of GLP-1 across the intestinal epithelium

Multistage pH-responsive mucoadhesive nanocarriers prepared by aerosol flow reactor technology: A controlled dual protein-drug delivery system

“…Chitosan, a positively charged biopolymer, has been shown to enhance the interaction with the mucus layer in the intestinal epithelia [6,11]. Possible detachment of the biopolymer from the NPs' surface before reaching the site of action can lead to the absence of desired therapeutic effects.…”

“…For these studies, Caco-2 and HT29-MTX cells were seeded in Lab-Tek™ 8-chamber slides (Thermo Fisher Scientific, USA) individually and as co-cultures (at a ratio 9:1, Caco-2/HT29-MTX) [6,11] glutaraldehyde at room temperature for 20 min. The cell monolayers were treated and prepared in copper grids, as described elsewhere [11].…”

“…The NPs were then centrifuged at 27,600 × g and washed once with 1 mL of Milli-Q water. The drug loading of GLP-1 was calculated by indirect method using the equation given elsewhere [6]. DPP4 inhibitor was loaded into the polymeric matrix that coated the CSUn NPs.…”

Design and statistical modeling of mannose-decorated dapsone-containing nanoparticles as a strategy of targeting intestinal M-cells

“…49,50 Optimization and characterization of the preparation procedure of nanoparticles was performed using a Box-Behnken experimental design. This response surface methodology was selected because, among other advantages, it allows avoiding extreme combinations of the studied factors.…”

In vitro assays for hazard identification of nanoparticles

“…Limited uptake was observed: only aft er 90-day exposure to 2500 mg/kg bw elevated Si levels were found in spleen . More studies are available that focussed on model polystyrene nanoparticles, and they generally highlight the dependence of uptake and accumulation of polystyrene nanoparticles on several factors, including their size, surface charge, and type of coating material (Araujo et al 2014, Hillery et al 1994, Hillyer and Albrecht 2001, Hussain and Florence 1998, Hussain et al 1997, Jani et al 1989. In general, smaller polystyrene nanoparticles were taken up across the GI tract to a higher extent than the larger ones (Jani et al 1990); the non-ionic more than the carboxylated ones (Jani et al 1989) and 407 poloxamer-coated more than 188 poloxamer-coated nanoparticles Florence 1996, Hussain et al 1997).…”