The impact of mesenchymal stem cells on doxorubicin‐induced testicular toxicity and progeny outcome of male prepubertal rats

Abstract

Background: Many therapies to treat cancer are gonadotoxic and can lead to infertility. New strategies to diminish the side effects and protective plans during and after chemotherapy are needed. Therefore, bone marrow mesenchymal stem cells (BM-MSCs) as a novel solution were investigated against doxorubicin (Dox)-induced toxicity in rat testes. Methods: Forty male albino prepubertal rats were divided into four groups, 10 rats per each group. The first was injected intraperitoneally with saline as control. The second group was injected intravenously with a single dose of BM-MSCs (2 × 10 6 cells). The third was injected intraperitoneally with a single dose of Dox (5 mg/kg b.wt). The fourth was injected with both Dox and BM-MSCs as previously mentioned. Rats were cohabited each separately with an untreated adult female after 8 weeks of treatment to examine Dox effects on male's fertility. Results: BM-MSCs counteract the deleterious effects of Dox on body, testicular weight as well as sperm quality by increasing sperm concentration and reducing the rate of abnormal sperm. BM-MSCs reduced significantly the testicular oxidative stress by reducing the elevated level of malondialdehyde and increasing the antioxidant capacity. Histologically, the testicular atrophy, severe damage of spermatogenesis and the significant reduction of the diameter and germinative cell layer thickness of the seminiferous tubules caused by Dox were significantly recovered after administration of the BM-MSCs. Conclusion: BM-MSCs have a significant role in restoring the structural efficiency of male reproductive system in rats after Dox treatment. K E Y W O R D Sbone marrow derived-mesenchymal stem cell, doxorubicin, male fertility, oxidative stress, testicular toxicity

Neuroprotective Potential of Bone Marrow-Derived Mesenchymal Stem Cells Following Chemotherapy

“…Different strategies have been used to ameliorate the adverse effects of CP, including its combination with other drugs, either chemical or natural products [6,7]. However, recent advances in regenerative medicine have focused on mesenchymal stem cells (MSCs) as a therapeutic option for various types of organ toxicity induced by chemotherapy [8][9][10][11] as well as different models of brain injury [12][13][14].…”

Comparison of the protective effects of curcumin and caffeic acid phenethyl ester against doxorubicin‐induced testicular toxicity

“…Abdelaziz et al (2019) revealed that MDA levels, which are indicators of lipid peroxidation, were increased in DOX-induced testicular toxicity and there was decreased total antioxidant capacity Cabral et al (2018). showed that MDA levels in the DOX group were significantly increased in DOX-induced testicular dysfunction in rats Benzer et al (2018).…”

Can mesenchymal stem cells ameliorate testicular damage? Current researches

“…Morphologically, testicular atrophy due to Dox, diameter of seminiferous tubules and germinative cell layer thickness were significantly reduced after BM-MSC transplantation. BM-MSCs were effective in restoring the structural efficiency of the reproductive system in testicular injury [44].…”