The impact of medium composition and photosynthetically active radiation level on the initial in vitro growth and production of flavonoids of Vernonia condensata Baker

Rodrigues, Fabíola Rebouças; Bispo, Dayse Alessandra Almeida Silva; Brandão, Hugo Neves; Soares, Taliane Leila; Almeida, Weliton Antônio Bastos de; Santana, José Raniere Ferreira de

doi:10.1016/j.bcab.2019.101063

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…Therefore, the sampling time between the spring and autumn had a 5-day difference in order to eliminate the effect of the slow growth of waxy corn kernels in the autumn. Water deficit and low humidity would also affect the accumulation of metabolite in cereals [ 22 ], as well as the PAR level, which affected the distribution and biosynthesis of secondary metabolites in plants [ 23 ]. High accumulation of anthocyanin was seen in corns induced by UV radiation, cold temperature, and water stress [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

The Accumulation and Biosynthesis of Anthocyanin in Black, White, and Yellow Waxy Corns (Zea mays L. sinensis kulesh) during Kernel Maturation

Liu

Shan

et al. 2023

Foods

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Waxy corn kernels with different colors have high phenolic content and good application potential in medicine and food healthcare. In our work, the content changes of phenolic and anthocyanins profiles were related to genes in the anthocyanin biosynthesis pathway, and the antioxidant activities of three different colors of waxy corn kernels (black, white, and yellow) were determined during kernel development. Results showed that growing temperature and light intensity could affect the accumulation of phytochemicals and antioxidant activities in waxy corns during maturation. Phenolic and antioxidant activities decreased over kernel maturation, and spring had higher nutrition levels during the best harvest time (20 and 25 days after pollination in the spring and autumn, respectively) for waxy corns. Cyanidin-3-O-glucoside and pelargonidin-3-O-glucoside were the main anthocyanins detected in the black waxy corns. The contents of cyanidin are higher than pelargonidin followed by peonidin in the autumn, while on the other hand, pelargonidin had a slightly higher content compared to cyanidin in the spring. DFR, CF1, and ANS were the key genes affecting anthocyanin accumulation. This work provided information on the best harvest time for the pigment of waxy corn in order to achieve relatively high phenolic profiles and antioxidant activities. It also illustrated the possible relationship between weather conditions, gene expression levels, and phenolic content during kernel development.

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Section: Discussionmentioning

confidence: 99%

The Accumulation and Biosynthesis of Anthocyanin in Black, White, and Yellow Waxy Corns (Zea mays L. sinensis kulesh) during Kernel Maturation

Liu

Shan

et al. 2023

Foods

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“…In plant cell and organ cultures, sucrose serves as an energy source for cells and organ proliferation, growth, and metabolite production. Thus, the addition of sucrose to the medium leads to an increase in phenolic and flavonoid compounds in Vernonia condensata [ 47 ]. The effect of low or high sucrose concentrations on GI and DW parameters on G. dinarica hairy roots cannot be generalized since each hairy root clone reacted specifically.…”

Section: Discussionmentioning

confidence: 99%

Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Vinterhalter

Banjac

Vinterhalter

et al. 2021

Plants

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The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

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“…Species of the genus Vernonia are rich sources of sesquiterpene lactones. is group of compounds has been shown to be insect antifeedant, antifungal, cytotoxic, and antitumoral [30][31][32]. Numerous sesquiterpene lactones have been identified to be present in Vernonia amygdalina; these include vernolide, vernolepin, vernodalin, and hydroxyvernolide [28,[33][34][35].…”

Section: Introductionmentioning

confidence: 99%

“…Most research conducted so far has dwelt on the pharmacological potential of the genus Vernonia, including anti-infective, anti-inflammatory, antioxidant, and anticancer [30][31][32]. At the time of carrying out this research, there were a dearth of scientific reports on the activity of the stem-bark of Vernonia camporum on the above study conditions, except anecdotal reports from local herbalists.…”

Section: Introductionmentioning

confidence: 99%

Anthelminthic, Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and FTIR Analyses of Vernonia camporum Stem-Bark

Akoto

Acheampong

Boakye

et al. 2021

Journal of Chemistry

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Vernonia camporum is used ethnomedicinally to treat diseases such as malaria, fever, hypertension, mouth sores, pains, inflammation, and skin rashes in Africa. This study aims at investigating the biological activities (anthelminthic, anti-inflammatory, antioxidant, and antimicrobial) and separating some of the biologically active components in the methanol crude extract of the stem-bark of Vernonia camporum. Phytochemical screening, anthelminthic (Lumbricus terrestris (earthworm)), anti-inflammatory (egg albumin denaturation), antioxidant (DPPH and H2O2 scavenging and total antioxidant capacity (TAC)), and antimicrobial (agar and broth dilution method) assays were performed on the extracts. Column chromatography and FTIR spectroscopic analysis were employed to separate and analyze the least polar constituents of the methanol extract. The preliminary phytochemical analyses revealed the presence of secondary metabolites such as alkaloids, phenols, tannins, saponins, terpenoids, flavonoids, steroids, phytosterols, proteins and amino acids, phlobatannins, glycosides, cardiac glycosides, coumarins, and anthocyanins. The extracts (methanol and hexane) showed a higher concentration-dependent anthelminthic activity. The extracts exhibited high concentration-dependent anti-inflammatory activities with IC50 values of 35.83 ± 3.984 and 53.91 ± 5.8413 µg/mL, respectively. The IC50 values for methanol and hexane extracts in the DPPH assay were 17.70 ± 3.430 and 29.54 ± 1.5437 µg/mL, and those of the H2O2 assay were 243.20 ± 0.1528 and 352.20 ± 12.64 µg/mL, respectively. TAC results for methanol and hexane extracts were recorded as 31.592 ± 1.682 and 30.232 ± 0.445 gAAE/100 g, respectively. The extracts were observed to exhibit antimicrobial activity against test organisms with MICs ranging from 0.1953 to 25.00 mg/mL. Chromatographic separations gave four fractions. FTIR analysis indicated the presence of various functional groups in purified fractions of the methanol extract that confirms the presence of the phytochemicals identified in the screening test. The results indicate that both extracts of V. camporum possess anthelminthic, anti-inflammatory, antioxidant, and antimicrobial activities supporting the traditional usage of this plant.

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The impact of medium composition and photosynthetically active radiation level on the initial in vitro growth and production of flavonoids of Vernonia condensata Baker

Cited by 8 publications

References 42 publications

The Accumulation and Biosynthesis of Anthocyanin in Black, White, and Yellow Waxy Corns (Zea mays L. sinensis kulesh) during Kernel Maturation

The Accumulation and Biosynthesis of Anthocyanin in Black, White, and Yellow Waxy Corns (Zea mays L. sinensis kulesh) during Kernel Maturation

Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Anthelminthic, Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and FTIR Analyses of Vernonia camporum Stem-Bark

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