The impact of LuxF on light intensity in bacterial bioluminescence

Brodl, Eveline; Csamay, Alexandra; Horn, Christina; Niederhauser, Johannes; Weber, Hansjörg; Macheroux, Peter

doi:10.1016/j.jphotobiol.2020.111881

Cited by 12 publications

(8 citation statements)

References 41 publications

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“…Therefore, insertion of the lux CDABE operon into the genome is an alternative method. To increase the expression of the lux CDABE core gene in the genome, other LUX components may be added; for example, luxF and luxG enhance the luminescence intensity of bacteria [ 31 , 32 ]. Improving the luminous intensity of luxCDABE can further improve the sensitivity of the system.…”

Section: Discussionmentioning

confidence: 99%

Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology

Zhong

Huang

et al. 2022

Plant Methods

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Background Ralstonia solanacearum, one of the most devastating bacterial plant pathogens, is the causal agent of bacterial wilt. Recently, several studies on resistance to bacterial wilt have been conducted using the Arabidopsis-R. solanacearum system. However, the progress of R. solanacearum infection in Arabidopsis is still unclear. Results We generated a bioluminescent R. solanacearum by expressing plasmid-based luxCDABE. Expression of luxCDABE did not alter the bacterial growth and pathogenicity. The light intensity of bioluminescent R. solanacearum was linearly related to bacterial concentrations from 104 to 108 CFU·mL−1. After root inoculation with bioluminescent R. solanacearum strain, light signals in tomato and Arabidopsis were found to be transported from roots to stems via the vasculature. Quantification of light intensity from the bioluminescent strain accurately reported the difference in disease resistance between Arabidopsis wild type and resistant mutants. Conclusions Bioluminescent R. solanacearum strain spatially and quantitatively measured bacterial growth in tomato and Arabidopsis, and offered a tool for the high-throughput study of R. solanacearum-Arabidopsis interaction in the future.

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Section: Discussionmentioning

confidence: 99%

Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology

Zhong

Huang

et al. 2022

Plant Methods

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“…Most of the genes annotated in the PHI database belonged to the reduced virulence genes and unaffected pathogenicity genes, indicating that the pathogenic ability of the strain was weak or nonpathogenic. Most luminous bacteria are nonpathogenic[18], while two subspecies of Vibrio harveyi and Photobacterium damselae are pathogens of many aquatic organisms [19,20]. Many species of Vibrio and Photobacterium have multiple resistance to many common antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing and bioinformatics analysis of Photobacterium kishitanii FJ21

Yin

Liu

et al. 2022

Preprint

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Background: Photobacterium kishitanii FJ21 is isolated and purified in the laboratory, and it is a kind of gram-negative bacteria that can emit blue-green fluorescence under normal conditions. In order to understand the lux operon that has luminescence activity, the whole-genome sequencing and bioinformatics analysis of Photobacterium kishitanii were carried out by using Nanopore sequencing technology. The sequencing data were predicted by the secondary structure and tertiary structure of the protein encoded by lux genes, genome assembly, GC content, gene prediction and functional annotation, phylogenetic tree, genome collinearity analysis and secondary metabolite synthesis gene cluster prediction. Result: Sequencing results showed that there were luxC, luxD, luxA, luxB, luxF, luxE and luxG genes in Photobacterium kishitanii FJ21, and the protein encoded by lux genes had certain hydrophilicity. The genome of this strain contains a chromosome with a total length of 4853277bp and a GC content of 39.23%. There are 3141, 1769, 2472, 4070, 3514 and 1413 genes annotated in COG, KEGG, GO, Refseq, Pfam and TIGRFAMs databases, respectively. At the same time, three types of secondary metabolite gene clusters are predicted, namely RiPP-like, betalactone and arylpolyene. 870 genes were annotated in PHI database. The antibiotic resistance genes of the strain were annotated with CARD database. Conclusion: This study reported the whole genome sequence Photobacterium kishitanii FJ21, and the related research results will provide a basis for further study of lux genes, photogenic activities and mining of other functional genes of this strain.

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“…As shown in Figure 7, a total of 2472 genes were annotated for GO function, accounting for 59.84 % of the total number of predicted genes. GO function mainly divides them into molecular function, biological process and cellular component [36]. In molecular function, there are many genes annotated by molecular transducer activity, antioxidant activity and transporter activity.…”

Section: Go Function Classificationmentioning

confidence: 99%

Whole-genome sequencing and single acute toxicity of heavy metal to Photobacterium kishitanii FJ21

Yin

Liu

et al. 2022

Preprint

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In this study, the growth morphology of FJ21 strain was observed, and its 16S rRNA and whole genome were sequenced. Then, related software was used to make genome assembly, gene structure and function annotation, genome phylogenetic tree analysis, genome collinearity analysis and prediction of secondary metabolic gene cluster analysis. Finally, the single acute toxicity of five heavy metals to FJ21 strain was detected. There were luxC, luxD, luxA, luxB, luxF, luxE and luxG genes in FJ21, and the protein encoded by lux operon had certain hydrophilicity. The genome of this strain FJ21 contains a chromosome with a total length of 4853277bp and a GC content of 39.23%. The genome of FJ21 was compared with that of Photobacterium kishitanii ATCCBAA-1194, Photobacterium phosphoreum JCM21184, Photobacterium aquimaris LC2-065, Photobacterium malacitanum CECT9190, and Photobacterium carnosum TMW 2.2021. The average nucleotide identity (ANI), tetra nucleotide signatures (Tetra), comparative genome, and phylogenetic analysis proposed that FJ21 is a strain of Photobacterium kishitanii. In the acute toxicity test, the toxicity of heavy metals to the strain FJ21 is Pb(NO3)2 > ZnSO4·7H2O > CdCl2·2.5H2O > CuSO4·5H2O > K2Cr2O7.

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The impact of LuxF on light intensity in bacterial bioluminescence

Cited by 12 publications

References 41 publications

Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology

Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology

Whole genome sequencing and bioinformatics analysis of Photobacterium kishitanii FJ21

Whole-genome sequencing and single acute toxicity of heavy metal to Photobacterium kishitanii FJ21

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