The impact of low-volume uterine lavage on endometrial biopsy classification

Linton, J.K.; Sertich, Patricia L.

doi:10.1016/j.theriogenology.2016.03.028

Cited by 5 publications

(6 citation statements)

References 9 publications

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“…An increase in uterine tissue perfusion was observed within 4 h of the procedure but the uterine hemodynamic returned to baseline by 24 h, indicating that these procedures may induce a short acute inflammatory response. Although previous reports indicate that performing UL, induces endometrial irritation caused by the fluid (Brook, 1993) or by the device (Kasimanickam et al, 2005b), studies in mares (Ball et al, 1988, Linton andSertich, 2016) and women (Hannan et al, 2012) have demonstrated that UL did not induce significant morphological changes to the endometrial tissue (Koblischke et al, 2008). Cheong et al (2012) and Thome et al (2016) performed UL and CB 4 h. after insemination and found that the procedures did not affect the PR in treated mares (Brinsko et al, 1991).…”

Section: Discussionmentioning

confidence: 92%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

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In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

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Section: Discussionmentioning

confidence: 92%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

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“…Endometrial biopsies were taken within 15 min of UL. It should be stressed that it was already shown that UL prior to endometrial biopsy does not affect the number of neutrophils in endometrial vessels or tissues [16].…”

Section: Part Ii: Comparison Of Cytological Methods With Endometrial ...mentioning

confidence: 88%

“…Biopsies were obtained as detailed elsewhere [13,16]. The collected material was fixed in formalin, routinely processed and stained with Hematoxylin-Eosin.…”

Section: Endometrial Biopsiesmentioning

confidence: 99%

The advantages of using cytospins of uterine lavage fluid for the diagnosis of equine endometritis

Marcos

Ribeiro²,

Santos

et al. 2020

Theriogenology

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“…Linton and Sertich (21) resalta el amplio espectro de actividad in vitro que tiene la cefquinoma frente a bacterias como Streptococcus spp, Staphylococcus spp, Pseudomonas spp, Escherichia coli, entre otros (21); Los resultados encontrados en el presente estudio sobre el uso clínico reproductivo y la eficacia que presentan las infusiones intrauterinas con cefquinoma representan una nueva posibilidad sobre la terapia clínica de la endometritis bacteriana en yeguas, ya que debido al poco uso farmacológico por esta vía son bajas las tasas de resistencia bacteriana a este agente antibiótico.…”

Section: /7unclassified

Evaluación de la eficacia clínica de tres protocolos antibióticos en yeguas diagnosticadas con endometritis bacteriana

Rodríguez

Ruíz-Jaramillo

Ruiz-Buitrago

2021

Rev Colombiana Cienc Anim. RECIA

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La endometritis bacteriana es una de las causas más comunes de falla reproductiva en yeguas, generalmente asociada a agentes bacterianos como Streptococcus equi, Echerichia coli, Enterococcus spp, Klebsiella sp., entre otros. De manera común se utilizan terapias antimicrobianas a base de fármacos betalactámicos y aminoglucósidos infiltrados a nivel uterino o en terapia sistémica. Para el presente estudio se tiene como objetivo principal evaluar la eficacia clínica de tres protocolos antibióticos en yeguas con diagnóstico de endometritis bacteriana y verificar su desempeño reproductivo postratamiento. La muestra se realizó con 90 yeguas diagnosticadas con infección uterina. Mediante examen clínico reproductivo, vaginoscopia y ecografía. Se tomaron muestras de cultivo uterino por medio de hisopado, a su vez se realizó antibiograma. Las yeguas fueron divididas en tres grupos de tratamiento antibiótico asignados según el resultado del antibiograma: penicilina G procaínica (n=4), ceftiofur (n=10) y gentamicina (n=20). Posteriormente se evaluó la eficacia clínica y el desempeño reproductivo de las yeguas. De las tratadas con penicilina, 75% (3/4) se recuperaron y dos quedaron gestantes; de las tratadas con gentamicina, 75% (15/20) se recuperaron y 8 quedaron gestantes; en tanto que de las tratadas con ceftiofur, 70% (7/10) se recuperaron y a 5 se le confirmó gestación. Se concluye no hubo diferencias clínicas o reproductivas postratamiento por efecto del tipo de antibiótico empleado.

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The impact of low-volume uterine lavage on endometrial biopsy classification

Cited by 5 publications

References 9 publications

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

The advantages of using cytospins of uterine lavage fluid for the diagnosis of equine endometritis

Evaluación de la eficacia clínica de tres protocolos antibióticos en yeguas diagnosticadas con endometritis bacteriana

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