The Impact of Leukemia on the Detection of Short Tandem Repeat (STR) Markers

Alharbi, Sara F; Alamri, Asim; El-Shehawi, Ahmed M.

doi:10.7759/cureus.30954

Cited by 3 publications

(2 citation statements)

References 79 publications

(75 reference statements)

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“…A study by Alharbi et al [ 38 ] investigated the impact of leukaemia on the detection and stability of short tandem repeat (STR) markers. The study involved analysing DNA samples from 15 individuals with chronic myeloid leukaemia (CML) and 15 healthy controls.…”

Section: Effects Of Carcinomas On Genetic Fingerprintsmentioning

confidence: 99%

Effect of Carcinomas on Autosomal Trait Screening: A Review Article

Alhatim,

Abdullah,

Abu Bakar

et al. 2023

CIMB

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This review highlights the effect of carcinomas on the results of the examination of autosomal genetic traits for identification and paternity tests when carcinoid tissue is the only source and no other samples are available. In DNA typing or genetic fingerprinting, variable elements are isolated and identified within the base pair sequences that form the DNA. The person’s probable identity can be determined by analysing nucleotide sequences in particular regions of DNA unique to everyone. Genetics plays an increasingly important role in the risk stratification and management of carcinoma patients. The available information from previous studies has indicated that in some incidents, including mass disasters and crimes such as terrorist incidents, biological evidence may not be available at the scene of the accident, except for some unknown human remains found in the form of undefined human tissues. If these tissues have cancerous tumours, it may affect the examination of the genetic traits derived from these samples, thereby resulting in a failure to identify the person. Pathology units, more often, verify the identity of the patients who were diagnosed with cancer in reference to their deceased tumorous relatives. Genetic fingerprinting (GF) is also used in paternity testing when the alleged parent disappeared or died and earlier was diagnosed and treated for cancer.

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Section: Effects Of Carcinomas On Genetic Fingerprintsmentioning

confidence: 99%

Effect of Carcinomas on Autosomal Trait Screening: A Review Article

Alhatim,

Abdullah,

Abu Bakar

et al. 2023

CIMB

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“…Short tandem repeats (STRs), also known as Microsatellites are small sequences of DNA (two to six base pairs) which are tandemly repeated and these repeat units are different among individuals, making them highly distinguishable 1 and are highly useful and widely used in human identity testing applications. 2,3 the artifacts of polymerase slippages i.e stutter peaks, ultimately interfering with the analysis of chimerism pattern.…”

Section: Introductionmentioning

confidence: 99%

Densitometry of STR-PAGE for donor chimerism in acute leukemia’s: A simple method for routine use

Nayyar,

Ahmed

2024

Pak J Med Sci

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Objective: To evaluate a PCR based method of polyacrylamide gel electrophoresis of short tandem repeats and its quantification for detecting donor chimerism after haematopoietic stem cell transplantation in acute leukaemias. Methods: The descriptive study was conducted at Genetic Resource Centre (GRC) Lab Rawalpindi from Feb 2018 - Nov 2020. A total of twenty patients with acute leukaemias having undergone HSCT were selected and assessed for the analysis of chimerism status. DNA extraction from the whole blood was done by chelex method and short tandem repeats were amplified by using conventional STR- PCR assay. Electrophoresis was carried out and 6% polyacrylamide gels were used for the resultant amplified DNA products and then followed by their densitometry. These patients had undergone HSCT from Pakistan Institute of Medical Science and Armed Forces Bone Marrow Transplant Centre. Results: The peaks in the PAGE densitometry represented the donor chimerism in all post transplant samples of the patients. Conclusion: Our study showed that densitometry of STR PCR PAGE is a useful and cheaper method for demonstration of donor chimerism in acute leukaemia patients having undergone HSCT. Hence this method can be a valuable option in the monitoring of chimerism status in these patients and therefore helps in preventing graft failure by fast and early treatment strategies for these patients. doi: https://doi.org/10.12669/pjms.40.5.9216 How to cite this: Nayyar A, Ahmed S. Densitometry of STR-PAGE for donor chimerism in acute leukemia’s: A simple method for routine use. Pak J Med Sci. 2024;40(5):---------. doi: https://doi.org/10.12669/pjms.40.5.9216 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Forensic short tandem repeat markers alteration in cancerous tissues: a scoping review

Omar,

Hafiza,

Zafarina

et al. 2024

Egypt J Forensic Sci

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Background Short Tandem Repeats (STRs) are segments of DNA composed of a short sequence of nucleotides that repeat consecutively. These repeating sequences exhibit distinct lengths and nucleotide sequences among individuals, showcasing high variability and uniqueness. The STR profile remains consistent across all cells in an individual’s body. Nonetheless, changes in the STR profile have been documented in cancerous tissues. This scoping review aimed to investigate the occurrence and pattern of forensic STR markers alterations in cancerous tissues. We conducted a scoping review of the English-language publications published between 2002 and 2022 in the PubMed, Science Direct, and Scopus databases and a manual search of reference lists from reviewed papers. The review was carried out in compliance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews. Results Our search resulted in a total of 1,065 articles associating forensic STR studies with cancerous tissues. A total of 18 of these studies met our inclusion criteria. The D18S51 marker was most often found to be altered when associated with cancers such as breast, colorectal, gastric, gynaecology, and lung cancers. Following with that, FGA, VWA, D19S433, and D13S317 markers could as well be seen to have allelic alteration in cancerous tissues. Four other STR markers (TPOX, D7S820, D2S1338, and Penta D) could be potentially represented as stable STR markers in cancerous tissues. Conclusions According to our review, colorectal cancer tissue has the highest level of genomic instability compared to that of other cancer types. In summary, the genetic instability caused by faulty DNA mismatch repair processes in human carcinomas can pose challenges for forensic genotyping and DNA profile matching.

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The Impact of Leukemia on the Detection of Short Tandem Repeat (STR) Markers

Cited by 3 publications

References 79 publications

Effect of Carcinomas on Autosomal Trait Screening: A Review Article

Effect of Carcinomas on Autosomal Trait Screening: A Review Article

Densitometry of STR-PAGE for donor chimerism in acute leukemia’s: A simple method for routine use

Forensic short tandem repeat markers alteration in cancerous tissues: a scoping review

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