The impact of l-arginine supplementation on the enteral phase of experimental Trichinella spiralis infection in treated and untreated mice

Fadl, Hanaa O.; Amin, Noha M; Wanas, Hanaa; El-Din, Shimaa Saad; Ibrahim, Hussein; Aboulhoda, Basma Emad; BOCKTOR, NARDEEN

doi:10.1007/s12639-020-01245-1

Cited by 4 publications

(5 citation statements)

References 54 publications

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“…The results from the present study are in the same line with Salama et al (2021) who reported an improvement in architecture of intestine in T. spiralis infected mice and treated with albendazole and prednisolone. Additionally, Fadl et al (2020) recorded that the inflammatory cells infiltration and goblet cells were significantly reduced in T. spiralis infected mice which treated by albendazole in comparison to infected non treated mice.…”

Section: Discussionmentioning

confidence: 99%

“…Life cycle commences with ingestion of raw or under cooked meat that contain the muscle larvae, where the digestive juice in the stomach dissolves the capsulelike cyst which consequently release of the infective larvae that move to the intestine with subsequent penetration to its columnar epithelium (Diaz et al, 2020). Promptly, the larvae differentiate into adults, then, after mating the adult female worms release large number of newborn larvae that penetrate the intestine (Fadl et al, 2020). Next, the larvae convey through the circulatory system into the skeletal muscle cells ISSN (Print) 2786-0272 ISSN (Online) 2786-0280 NVVJ.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Clinicopathological alterations in mice experimentally infected with Trichenella spiralis and the Nematocidal effect of Tannic acid and Albendazole

Swilam

Nasreldin²,

Abd-Elrahman³

et al. 2022

New Valley Veterinary Journal

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Trichinella spiralis (T. spiralis) is one of the parasitic nematodes of a highly zoonotic importance. It infects various vertebrates, including human. In this study eighty (n =80) Swiss albino mice were experimentally infected by T. spiralis to assess the nematocidal activity of tannic acid (TA) and albendazole (ABZ) individually or in combination during the intestinal phase of T. spiralis. Mice were divided into 10 equal groups 8 mice for each, (control): noninfected but received saline, (ABZ): non-infected and treated with ABZ 50 mg/kg, (TA1000): non-infected and treated with TA1000 µg/ml, (TA2000): non-infected and treated with TA2000 µg/ml, (ABZ+TA2000): non-infected and treated with ABZ 50 mg/kg + TA2000 µg/ml , (TS): infected non treated, (TS+ABZ) group: infected and treated with ABZ 50mg/kg, (TS+TA1000): infected and treated with TA1000 µg/ml, (TS+TA2000): infected and treated with TA2000 µg/ml and (TS+ABZ+TA2000): infected and treated with ABZ 50mg/kg + TA2000 µg/ml. Mice were sacrificed at 7 th day post infection. Results demonstrated significant reductions in the adult worm count in TS+TA1000, TS+ABZ, TS+ TA2000 and TS+ABZ+TA2000 with efficacies of 91%, 92%, 92% & 99% respectively. Results were confirmed by histopathological examination of the targeted organs. The degree of necrosis was decreased in treated groups, associated with reduction in the percentage of eosinophils. Elevated concentrations of serum ALT, AST, and urea in the infected group were decreased in treated groups, while albumin and glucose were increased.It was concluded that the combined albendazole-tannic acid therapy had the highest effect on reducing parasite burden and restoring normal histological architecture.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of Clinicopathological alterations in mice experimentally infected with Trichenella spiralis and the Nematocidal effect of Tannic acid and Albendazole

Swilam

Nasreldin²,

Abd-Elrahman³

et al. 2022

New Valley Veterinary Journal

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“…Different studies have reported different efficacy rates for alben-dazole, according to the dose, duration of administration, timing of animal sacrifice and stage of infection. Fadl et al (2020) examined the effect of albendazole during the intestinal phase of infection. The drug was administered at a dose of 50 mg/kg on day 2 post-infection for 3 days and a reduction rate of 90.91 % was achieved.…”

Section: Discussionmentioning

confidence: 99%

“…Assessment of adult worm count in small intestine The small intestines of the sacrifi ced mice were removed, opened longitudinally, washed with saline and sectioned into small pieces and incubated at 37°C in phosphate buffered saline (PBS) for 2 hours. Then the adult worms were collected and counted under a dissecting microscope (Fadl et al, 2020) Assessment of larval count in muscle The rectus abdominus muscle of each mouse was dissected and incubated in 1 % pepsin and 1 % HCl in distilled water at 37°C for 2 hours with intermittent agitation using an electric stirrer. Coarse particles were fi rst removed from the digested product on a 50 mesh/inch sieve, then larvae were collected on a 200 mesh/inch sieve, washed twice and suspended in 150 ml tap water in a conical fl ask.…”

Section: Parasitological Methods For the Evaluation Of Parasite Burdenmentioning

confidence: 99%

Investigation of the effect of curcumin on oxidative stress, local inflammatory response, COX-2 expression, and microvessel density in Trichinella spiralis induced enteritis, myositis and myocarditis in mice

et al. 2022

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Summary Background Curcumin exerts anti-oxidant and anti-inflammatory properties that have proven to be of value in the management of several parasitic infections. Objective Investigation of the value of curcumin in the management of trichinosis either alone or as an adjuvant to albendazole. Methods Animals received either curcumin 150 mg/kg, curcumin 300 mg/kg, albendazole 50 mg/ kg or combined curcumin 150mg/kg and albendazole 50 mg/kg and were compared with control infected and non-infected mice. Estimation of intestinal and muscular parasitic load and blood malondialdehyde level, in addition to the histopathological examination of small intestine, skeletal muscle tissue and heart was performed. Also, assessment of the local expression of cyclooxygenase-2 enzyme (COX-2) and CD34 in these samples was done by immunohistochemistry. Results Curcumin was found efficient in reducing parasitic load. It also lowered serum MDA level, local COX-2 and CD34 expression. An evident anti-inflammatory effect of curcumin was observed in intestinal, skeletal muscle and cardiac muscle histopathological sections. Conclusion The anti-inflammatory, anti-oxidant and anti-angiogenic effects of curcumin can help to improve trichinellosis-induced pathology. Curcumin can therefore be of value as an adjuvant therapy to conventional antiparasitic agents and can also produce promising results when used alone at higher doses.

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“…The small intestines were opened longitudinally, washed with saline and cut into small pieces. Incubation at 37 °C was done in phosphate buffered saline (PBS) for 2 h. The adult worms were then collected and counted under a dissecting microscope ( Fadl et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Investigation of the effect of the calcium channel blocker, verapamil, on the parasite burden, inflammatory response and angiogenesis in experimental Trichinella spiralis infection in mice

Fadil

Mahmoud

El-Ahl

et al. 2022

Food and Waterborne Parasitology

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Trichinella spiralis larvae have very special characters that make them able to completely transform the function of the affected muscle cells towards a self-serving environment, offering them nourishment and protection via what is known as “nurse cells”. This setting may be affected by drugs that are used for the treatment of co-morbidities and co-infections as calcium channel blockers, which are widely used in clinical practice. In the present study, the effects of verapamil, ivermectin (IVM), and their combined administration on the parasitic burden, immuno-pathology and angiogenesis were investigated during experimental trichinellosis. Estimation of intestinal adult parasitic stages and muscle larvae was done. VEGF gene expression and CD31 immunohistochemical local expression were measured to investigate angiogenesis, in addition to histopathological examination to explore the extent of inflammation. Although verapamil did not have an effect on the adult worm count during the intestinal phase, it induced an anti-inflammatory effect on intestinal pathology. During the muscle phase, it was very effective in reducing the larval count by 93.78%. IVM effectively reduced the worm count by 85.34%, and the muscle larval count by 97.84%, while combined verapamil and IVM administration resulted in a significant reduction in both adult parasites by 69.5% and larval stages by 99%. Both verapamil and IVM and their combination induced a potent decrease in local CD31 protein expression and VEGF gene expression. The important role of calcium and calcium channels during the pathology of trichinellosis, in addition to the pivotal role of calcium on biological processes such as immunity and angiogenesis, make calcium-channel blockers promising candidates for drug repurposing in the management of helminthic infection.

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The impact of l-arginine supplementation on the enteral phase of experimental Trichinella spiralis infection in treated and untreated mice

Cited by 4 publications

References 54 publications

Evaluation of Clinicopathological alterations in mice experimentally infected with Trichenella spiralis and the Nematocidal effect of Tannic acid and Albendazole

Evaluation of Clinicopathological alterations in mice experimentally infected with Trichenella spiralis and the Nematocidal effect of Tannic acid and Albendazole

Investigation of the effect of curcumin on oxidative stress, local inflammatory response, COX-2 expression, and microvessel density in Trichinella spiralis induced enteritis, myositis and myocarditis in mice

Investigation of the effect of the calcium channel blocker, verapamil, on the parasite burden, inflammatory response and angiogenesis in experimental Trichinella spiralis infection in mice

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