The impact of irrigation protocols on epoxy sealer penetration depth in dentinal tubules. Study involving laser confocal microscopy

Abstract

The aim was to assess the impact of irrigation protocols ended with ethanol or chlorhexidine on AH Plus penetration into dentinal tubules. 45 root canals were prepared to ISO 40/04, divided into three groups and irrigated with three protocols: Group 1 (control): 5.25% NaOCl; Group 2: 40% CA (citric acid), 5.25% NaOCl, 40% CA, water, ethanol; Group 3: 40% CA, 5.25% NaOCl, 40% CA, water, 2% chlorhexidine. Canals were filled using vertical condensation technique with gutta-percha and fluorescein-stained AH Plus sealer. After 72 h, 1 mm thick cross-sections were cut at 2, 5, 8 mm from the apex. Confocal laser microscope was used to measure the sealer penetration into dentinal tubules. Mean depth of sealer penetration (in micrometres) was 107, 131, 170 (Group 1); 146, 233, 317 (Group 2); 185, 301, 542 (Group 3); in apical, middle and coronal parts, respectively. Irrigation protocol ended with chlorhexidine resulted in the deeper sealer penetration compared with alcohol.

SEM Evaluation of Sealer Penetration into Dentinal Tubules with and without Ultrasonic Irrigation Activation (An-In Vitro study)

“…The exerted pressure generated during the lateral compaction of gutta-percha, resulted in entrance of the sealer even into the partially opened dentinal tubules. (24) The Adseal sealer used in the present study is epoxy resin-based sealer where it has good physical and sealing properties, but the main disadvantage is its hydrophobic nature. While CeraSeal has better physiochemical properties in addition to its hydrophilic nature and antibacterial effect.…”