The Impact of Implementation of Rapid<i>Quick</i>FISH Testing for Detection of Coagulase-Negative Staphylococci at a Community-Based Hospital: Table 1

Koncelik, Denise L.; Hernandez, J.

doi:10.1093/ajcp/aqv005

Cited by 11 publications

(14 citation statements)

References 17 publications

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“…Indeed, most of the studies that have favorable outcomes from implementing PNA FISH ® or QuickFISH ® , incorporated an AMS program. 12,19 In our study, all of the participating hospitals had active AMS programs as demonstrated by previous studies done by our group (data not published).…”

Section: Discussionsupporting

confidence: 69%

“…Our results are in accordance to many other studies that have also reported that FISH methods provide faster results compared to conventional cultures. 6,9,12,13 Ly and colleagues' 11 also found a reduction in the duration of antibiotic treatment (-2.0 days; p = 0.01), as well as Forrest et al which evaluated the clinical utility of enterococcal PNA FISH ® , showing less time to effective therapy (1.3 vs. 3.1 days). 9 Although in our study a difference in mortality of 22% among the groups was observed (QuickFISH ® 13% versus BC 35%), it is not possible to establish a statistical association between the implementation of the QuickFISH ® and its direct impact on mortality; the study sample is a limitation as well as its design ; furthermore, part of difference in mortality between the groups could be explained in part to the higher proportion of patients with candidemia in the control group which have been shown to have significantly higher mortality compared to bacterial infections.…”

Section: Discussionmentioning

confidence: 85%

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Impact of a Rapid Molecular Diagnostic Test for the Identification of Bloodstream Infections in Intensive Care Units: Experience from a Developing Country

Salcedo

Hernández-Gómez

Pallares

et al. 2019

Preprint

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Background Rapid diagnostics have been demonstrated to be a crucial component of antimicrobial stewardship programs. However, most of the studies have been conducted in developed countries where health-care facilities have 24/7 microbiology laboratories. Colombia is an example of a developing country with limited resources in which hospitals are not able to implement a 24/7 model and samples are usually processed once a day. Here, we compared time to pathogen identification by QuickFISH® with conventional cultures and its effect on decrease duration of therapy in critical patients. Methods A multicenter, ambispective cohort study was conducted in four high-complexity ICU hospitals between 2016-2017. Adult patients admitted to the ICU with positive blood cultures and signs of systemic inflammatory response syndrome were included in the study. Patients with bloodstream infections identified by either QuickFISH® or PNA FISH® were observed prospectively and compared with those patients with bloodstream infections identified by conventional blood cultures alone who were analyzed retrospectively. Duration of treatment, time to final reports and survival rate were compared between the two groups. Additionally, the performance of the molecular test was compared with the conventional blood culture. Results A total of 153 patients were included in the study. Among them, 72 (47%) were in the QuickFISH® / PNA FISH® group and 81 (53%) in the conventional blood culture group. 87% of the patients had a bacteria identified (n=133) and 13% (n=20) a candida. QuickFISH® / PNA FISH® had 96% (89%-100%) concordance with blood culture. The microbiological identification report was 26 hours faster in the QuickFISH® group than in blood culture group (29 hours vs. 55 hours; p = 0.0001). The duration of antimicrobial therapy was 3.2 days shorter in the QuickFISH® group compared to the BC group (13.7 days vs. 16.9 days; p = 0.026). Conclusions Molecular diagnostic methods such as QuickFISH® reduce the time to final reports as well as the duration of therapy in ICU patients with bloodstream infections. Despite having more impact in 24/7 laboratories, QuickFISH® methods may be a promising diagnostic tool in developing countries if incorporated with antimicrobial stewardship programs.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 85%

Impact of a Rapid Molecular Diagnostic Test for the Identification of Bloodstream Infections in Intensive Care Units: Experience from a Developing Country

Salcedo

Hernández-Gómez

Pallares

et al. 2019

Preprint

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“…Together with culture and NA amplification techniques, this has potential for a ‘staging’ of biofilm infections in the future and the development of specific therapeutic strategies for the different stages. PNA FISH is becoming more widespread in diagnostic labs with the advent of validated kits designed for high‐throughput examination following blood culture. This approach may be useful in the case of specific microbial pathogens such as Staphylococcus aureus and in cases where a faster time to positivity is desirable . This approach may also help to target appropriate antimicrobial therapy .…”

Section: Diagnostic Methodsmentioning

confidence: 99%

“…• PNA FISH is becoming more widespread in diagnostic labs with the advent of validated kits designed for high-throughput examination following blood culture. This approach may be useful in the case of specific microbial pathogens such as Staphylococcus aureus and in cases where a faster time to positivity is desirable (85). This approach may also help to target appropriate antimicrobial therapy (86,87).…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Microbiological diagnosis of device‐related biofilm infections

Larsen

Lorenzen

et al. 2017

APMIS

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Medical device-related infections cause undue patient distress, increased morbidity and mortality and pose a huge financial burden on healthcare services. The pathogens are frequently distributed heterogeneously in biofilms, which can persist without being effectively cleared by host immune defenses and antibiotic therapy. At present, there is no 'gold standard' available to reveal the presence of device-related biofilm infections. However, adequate sample collection and logistics, standardised diagnostic methods, and interpretation of results by experienced personnel are important steps in efficient diagnosis and treatment of these infections. The focus of this mini review is on prosthethic joint and cardiovascular implantable device infections, which exemplify permanent devices that are placed in a sterile body site. These device-related infections represent some of the most challenging in terms of both diagnosis and treatment.

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“…This technique requires prior Gram staining and subsequently the slide is viewed under a fluorescence microscope, turn-around time 1.5-3 h. Recent introduction of the next-generation test, the QuickFISH V R , the time for processing has been further reduced and the turn-around time is currently around 0.5 h. A number of different QuickFISH V R assays have been developed and validated, each capable of detecting up to a maximum of three different species per assay. Staphylococcus QuickFISH V R differentiates Staphylococcus aureus from coagulase-negative staphylococci (CoNS) [9][10][11][12], Enterococcus QuickFISH V R for identification of Gram-positive cocci in cluster differentiates Enterococcus faecalis from other Enterococcus spp., including Enterococcus faecium [12][13][14], the Gram-Negative QuickFISH V R which differentiates Escherichia coli from Pseudomonas aeruginosa and Klebsiella pneumonia [12], and finally the Candida QuickFISH V R assay differentiating Candida albicans from Candida glabrata and Candida parapsilosis [15].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of QuickFISH and maldi Sepsityper for identification of bacteria in bloodstream infection

Enroth

Retz

Andersson

et al. 2019

Infectious Diseases

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Background: Early detection of bacteria and their antibiotic susceptibility patterns are critical to guide therapeutic decision-making for optimal care of septic patients. The current gold standard, blood culturing followed by subculture on agar plates for subsequent identification, is too slow leading to excessive use of broad-spectrum antibiotic with harmful consequences for the patient and, in the long run, the public health. The aim of the present study was to assess the performance of two commercial assays, QuickFISHV R (OpGen) and Maldi Sepsityper TM (Bruker Daltonics) for early and accurate identification of microorganisms directly from positive blood cultures. Materials and methods: During two substudies of positive blood cultures, the two commercial assays were assessed against the routine method used at the clinical microbiology laboratory, Unilabs AB, at Skaraborg Hospital, Sweden. Results: The Maldi Sepsityper TM assay enabled earlier microorganism identification. Using the cutoff for definite species identification according to the reference method (>2.0), sufficiently accurate species identification was achieved, but only among Gram-negative bacteria. The QuickFISH V R assay was time-saving and showed high concordance with the reference method, 94.8% (95% CI 88.4-98.3), when the causative agent was covered by the QuickFISH V R assay. Conclusions: The use of the commercial assays may shorten the time to identification of causative agents in bloodstream infections and can be a good complement to the current clinical routine diagnostics. Nevertheless, the performance of the commercial assays is considerably affected by the characteristics of the causative agents.

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The Impact of Implementation of RapidQuickFISH Testing for Detection of Coagulase-Negative Staphylococci at a Community-Based Hospital: Table 1

Abstract: The QuickFISH assay, with its ability to provide timely and actionable results nearly simultaneously with the Gram stain, in conjunction with an effective antimicrobial stewardship program, has been adopted as standard of care at our community-based hospital.

Cited by 11 publications

References 17 publications

Impact of a Rapid Molecular Diagnostic Test for the Identification of Bloodstream Infections in Intensive Care Units: Experience from a Developing Country

Impact of a Rapid Molecular Diagnostic Test for the Identification of Bloodstream Infections in Intensive Care Units: Experience from a Developing Country

Microbiological diagnosis of device‐related biofilm infections

Evaluation of QuickFISH and maldi Sepsityper for identification of bacteria in bloodstream infection

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