The impact of immunological and biomolecular investigations on the outcome of children with acute lymphoblastic leukemia - experience of III^rd Paediatric Clinic Timisoara

Abstract

Introduction. The unsatisfactory results of the survival in patients with

Do we really need genetic tests in current clinical practice?

“…For evaluation of chromosome number and morphology, GTG banding (G-banding by using trypsin for digestion and Giemsa staining) should be applied. At least 20 metaphases should be counted and karyotypes should be reviewed in compliance with the latest International System for Cytogenetic Nomenclature (ISCN) recommendations (1). Some rather pessimistic people considered cytogenetics to be outdated, but the reality is different.…”

Exploring Clinical and Biological Features of Premature Births among Pregnant Women with SARS-CoV-2 Infection during the Pregnancy Period

“…The fear of short-term death has trumped the worry about the long-term effects of steroid usage, while the convenience of availability, cheap cost, and apparent short-term therapeutic benefit have led to the irresponsible use of steroids, including self-medication, even in moderate COVID-19 patients. In COVID-19 patients, the administration of corticosteroids has increased the incidence of elevated serum glucose levels and the acute consequences of diabetic ketoacidosis that can negatively influence pregnancy and determine future complications among newborns [49,50].…”

Genetic testing in pediatrics - a narrative essay of challenges and possibilities in Romania

“…Cytogenetic analysis of hematological disorders (like acute lymphoblastic leukemia ALL, acute myeloid leukemia AML, myelodysplastic syndrome MDS, etc.) for identification of chromosomal abnormalities (aneuploidy and chromosomal rearrangements such as deletions, translocations) has become essential for diagnosis, risk-stratification, management of these diseases and monitoring of treatment response (15). Cryptic cytogenetic alterations may be revealed by CMA and by MLPA methods (16).…”

“…Cryptic cytogenetic alterations may be revealed by CMA and by MLPA methods (16). Fusion genes, the result of chromosomal translocation, are important for risk stratification and may be used as prognostic markers in children with ALL (15). Genomic studies in ALL are of clinical importance and are expected to improve diagnosis, monitoring of minimal residual disease (MRD), early detection of relapse, and for implementation of targeted therapy (17).…”