The impact of hyperthermic chemotherapy on human gastric cancer cell lines: Preliminary results

Abstract

Abstract. In this preliminary study, we evaluated the impact of hyperthermia (HT) and hyperthermic chemotherapy (HTCT) on six human gastric cancer cell lines and explored the mechanisms of cell-killing effect under HTCT. Treatment conditions were categorized into 4 modes: i) normothermic control (NT), ii) HT, iii) normothermic chemotherapy (NTCT) and iv) HTCT. According to the data of MTT and LM observations, isolated HT only temporarily inhibited cell proliferation and had no cell-killing effect on gastric cancer cell lines employed in our study except for SNU-1. Combining with HT enhanced the cytotoxicity of CDDP in all gastric cell lines and the concentration inhibiting cell proliferation and inducing cell death of HTCT was much lower than that of NTCT. There was a synergistic effect of HT and chemotherapy on inhibiting proliferation in each cell line in a certain range of CDDP concentration. The data of TEM and FCM proved that HTCT induced cell death with two modesapoptosis and necrosis, and apoptosis was the major type. Microarray illustrated that, under HTCT, a total of 58 gene expressions were regulated according to the filtering criteria, including 10 extra genes with an expression change below the threshold or even unchanged when treated with either HT or CDDP alone. Five of these 10 genes were verified by QRT-PCR. These genes may include the target genes for the enhancing effect of HT on chemotherapy and their effects should be further validated by functional analysis.

Importance of genetic background for risk of relapse shown in altered prefrontal cortex gene expression during abstinence following chronic alcohol intoxication

“…A comparison is shown between the level of expression difference identified by qPCR vs. that identified in the array analysis in Table 1 with confirmation of 28 out of 32 genes, or ~88%. This rate of confirmation is consistent with our previous array analysis that used a similar approach to detect modest expression differences (Hashimoto and Wiren, 2008), and the confirmation rate is high relative to other array platforms and analytical approaches (see Treadwell et al, 2004, see Tang et al, 2006). …”

Neurotoxic Consequences of Chronic Alcohol Withdrawal: Expression Profiling Reveals Importance of Gender Over Withdrawal Severity

“…We used an uncorrected P-value to decrease the chance of excluding EtOH-regulated transcripts (false negatives). Confirmation of differences by qRT-PCR analysis (Supplementary Methods and Supplementary Figure 2 in supporting information online) with samples derived from animals distinct from those employed for the microarray hybridizations indicated a percent positive that was consistent with reports in the literature using other array platforms and analytical approaches (see Tang et al, 2006;Treadwell and Singh, 2004). We selected genes for confirmation from pathways identified as influenced by withdrawal, particularly those involved in cell death and anti-inflammatory processes.…”

Understanding the addiction cycle: A complex biology with distinct contributions of genotype vs. sex at each stage

“…Prior confirmation of expression differences identified by hybridization using qPCR was ~88% (Hashimoto et al, 2011), similar to our previous finding of an ~80% confirmation rate (Hashimoto and Wiren, 2008). This rate of confirmation is high relative to other array platforms and analytical approaches (see Treadwell and Singh, 2004, Tang et al, 2006). Overall, nearly 20% of genes examined demonstrated significant ethanol regulation during any time point in sex- and line-specific comparisons (819 of 4696 genes queried).…”