The impact of high-glucose or high-fat diets on the metabolomic profiling of mice

Xie, Dadi; Zhang, Yanbo; Guo, Yujin; Xue, Xianzhong; Zhao, Shiyuan; Geng, Chunmei; Li, Yuanyuan; Yang, Rui; Gan, Yulin; Li, Hanbing; Ren, Zhongfa; Jiang, Pei

doi:10.3389/fnut.2023.1171806

Cited by 3 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results showed a direct correlation of fecal L-glutamic acid levels with e-WAT and body weight gain, consistent with previous studies [34] . L-(+)-Aspartic acid, associated with lipogenesis and inflammation, was reported to be significantly enriched in the serum of obese rats and in HFD mice.…”

Section: Discussionsupporting

confidence: 93%

Chlorantraniliprole exposure aggravates high-fat diet-induced metabolic disorders in mice by regulating gut microbiota and its metabolites

Gu,

Lin,

Yang

et al. 2024

Food Science and Human Wellness

View full text Add to dashboard Cite

Food contaminants, particularly insecticides, are important factors contributing to obesity and other adverse effects. As the most widely used diamide insecticide worldwide, chlorantraniliprole (CP) is ubiquitous in food and the environment. However, the influence of CP on obesity and the gut microbiota remains unknown. In this study, we administered CP/carboxymethyl cellulose sodium to C57BL/6J mice with a high-fat diet (HFD) via gavage for 13 weeks. The CP exposure induced significant increases in body weight gain, fat mass, serum total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C). Moreover, CP aggravated the imbalance in the gut microbiota by increasing the relative abundance of obesity-associated bacteria and reducing the relative abundance of beneficial bacteria. Based on untargeted metabolomics analysis, CP was found to be involved in the regulation of pathways including 'alanine, aspartate and glutamate metabolism' and 'arginine and proline metabolism'. Notably, CP exposure primarily induced alterations in microbial metabolites within these pathways, such as L-(+)-aspartic acid and L-glutamic acid. Additionally, individual metabolites, such as the lipid mediator (oleoyl ethanolamide), also demonstrated alterations upon CP exposure. Furthermore, Spearman correlation analysis revealed several noteworthy associations between microbial alterations, metabolite changes, and phenotypes. The results of the study demonstrate a connection between microbiota, metabolites, and the effects of CP exposure on HFD-induced obesity, elucidating the critical role of the gut microbiota and its metabolites in the toxic effects of CP.

show abstract

Section: Discussionsupporting

confidence: 93%

Chlorantraniliprole exposure aggravates high-fat diet-induced metabolic disorders in mice by regulating gut microbiota and its metabolites

Gu,

Lin,

Yang

et al. 2024

Food Science and Human Wellness

View full text Add to dashboard Cite

show abstract

“…The connection between some metabolites such as myristic acid, N -acetylneuraminic acid or phenylalanine and energy homeostasis has previously been established. HFD consumption significantly increased the level of myristic acid in the liver of mice [ 25 ]. The ingestion of N -acetylneuraminic acid attenuated HFD-induced inflammation and oxidative stress, and attenuated hypercoagulation in HFD-induced hyperlipidemic rats [ 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Citrus p-Synephrine Improves Energy Homeostasis by Regulating Amino Acid Metabolism in HFD-Induced Mice

Bai,

Tan,

Tang

et al. 2024

Nutrients

View full text Add to dashboard Cite

p-Synephrine is a common alkaloid widely distributed in citrus fruits. However, the effects of p-synephrine on the metabolic profiles of individuals with energy abnormalities are still unclear. In the study, we investigated the effect of p-synephrine on energy homeostasis and metabolic profiles using a high fat diet (HFD)-induced mouse model. We found that p-synephrine inhibited the gain in body weight, liver weight and white adipose tissues weight induced by HFD. p-Synephrine supplementation also reduced levels of serum total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) but not to a statistically significant degree. Histological analysis showed that HFD induced excessive lipid accumulation and glycogen loss in the liver and adipocyte enlargement in perirenal fat tissue, while p-synephrine supplementation reversed the changes induced by HFD. Moreover, HFD feeding significantly increased mRNA expression levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) and reduced the mRNA expression level of interleukin-10 (IL-10) compared to the control group, while p-synephrine supplementation significantly reversed these HFD-induced changes. Liver and serum metabolomic analysis showed that p-synephrine supplementation significantly altered small molecule metabolites in liver and serum in HFD mice and that the changes were closely associated with improvement of energy homeostasis. Notably, amino acid metabolism pathways, both in liver and serum samples, were significantly enriched. Our study suggests that p-synephrine improves energy homeostasis probably by regulating amino acid metabolism in HFD mice, which provides a novel insight into the action mechanism of p-synephrine modulating energy homeostasis.

show abstract

Metabolic Profiling Changes Induced by Fermented Blackberries in High-Fat-Diet-Fed Mice Utilizing Gas Chromatography–Mass Spectrometry Analysis

Park,

Kim,

Lee

et al. 2024

Biology

View full text Add to dashboard Cite

The aim of this study was to investigate the metabolic changes associated with the anti-obesity effects of fermented blackberry extracts in the liver tissues of high-fat-diet-fed mice using mass spectrometry-based metabolomics analysis. C57BL/6J mice were divided into eight groups: normal-diet-fed mice, high-fat-diet-fed mice, high-fat diet treated with blackberry extract, high-fat-diet mice treated with blackberry fermented by L. plantarum, and high-fat diet with blackberry fermented by L. brevis. After 12 weeks, the high-fat-diet group exhibited a greater increase in liver weight compared to the control group, and among the groups, the group administered with blackberry fermented with L. plantarum showed the most pronounced reduction in liver weight. As the primary organ responsible for amino acid metabolism, the liver is crucial for maintaining amino acid homeostasis. In our study, we observed that the levels of several essential amino acids, including isoleucine and valine, were decreased by the high-fat diet, and were recovered by administration of blackberry extract fermented with L. plantarum. Our results demonstrated the potential of blackberry extract fermented with L. plantarum as a functional material for metabolic disorders by restoring some of the amino acid metabolism disturbances induced by a high-fat diet.

show abstract

The impact of high-glucose or high-fat diets on the metabolomic profiling of mice

Cited by 3 publications

References 38 publications

Chlorantraniliprole exposure aggravates high-fat diet-induced metabolic disorders in mice by regulating gut microbiota and its metabolites

Chlorantraniliprole exposure aggravates high-fat diet-induced metabolic disorders in mice by regulating gut microbiota and its metabolites

Citrus p-Synephrine Improves Energy Homeostasis by Regulating Amino Acid Metabolism in HFD-Induced Mice

Metabolic Profiling Changes Induced by Fermented Blackberries in High-Fat-Diet-Fed Mice Utilizing Gas Chromatography–Mass Spectrometry Analysis

Contact Info

Product

Resources

About