The Impact of Genetic Variability of TGF-Beta Signaling Biomarkers in Major Craniofacial Syndromes

Yapijakis, Christos; Davaria, Sofianna; Gintoni, Iphigenia; Chrousos, George P.

doi:10.1007/978-3-031-31978-5_16

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…These histological and micro-CT analyses again strongly support longer term studies in regenerative endodontics to fully assess whether healing is prevented or simply delayed. This is particularly relevant to our study and to dental clinicians given the research showing that TGFβ signaling plays a large role in regulating the dentin-pulp complex [3,5,6,9,50] and that disrupted TGFβ signaling results in oral-dental anomalies [51,52] and delayed oral wound healing [53]. It is important to note that the dentin proteome between the Tgfbr2 cko and WT mice should be nearly identical due to the Cre recombinase suppression until the experimental timeline.…”

Section: Discussionmentioning

confidence: 86%

Impaired Tertiary Dentin Secretion After Shallow Injury in Tgfbr2-Deficient Dental Pulp Cells Is Rescued by Extended CGRP Signaling

Stanwick,

Fenesha,

Hamid

et al. 2024

Preprint

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Introduction: Transforming growth factor beta receptor 2 (Tgfbr2) signaling regulates odontoblast differentiation, dentin mineralization, and the paracrine signaling that guides sensory innervation in developing mouse molars. We hypothesized that Tgfbr2 also regulates the neuro-pulpal responses to injury to activate axon sprouting and tertiary dentin secretion. Methods: We per-formed a timed deletion of Tgfbr2 using tetracycline-responsive Osterix-Cre;Tgfbr2f/f (Tgfbr2^cko) mice, which allowed them to develop normally. At 3 months, we drilled a shallow hole on the mesial side of the first mandibular molar (M1) in both control and Tgfbr2^cko mice. Hemi-mandibles were collected 4, 8, 21, and 56 days post-injury (dpi). In situ hybridization (ISH) for Sp7 was performed to confirm Osterix-Cre transcription. Microcomputed tomography (micro-CT) imaging and histology were used to examine the tertiary dentin, and immunofluorescence was used to visualize CGRP+ axons following the injury. Results: Tgfbr2 deletion was inferred by Sp7 ISH. Micro-CT and histology indicated a lower dentin volume in 21 dpi Tgfbr2^cko M1s compared to WT M1s, but the volume was comparable by 56 dpi. The duration of axon sprouting was longer in injured Tgfbr2^cko M1s compared to WT M1s. Conclusions: These results suggest that sensory afferents may support dentin repair in Tgfbr2-deficient odontoblasts.

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Section: Discussionmentioning

confidence: 86%

Impaired Tertiary Dentin Secretion After Shallow Injury in Tgfbr2-Deficient Dental Pulp Cells Is Rescued by Extended CGRP Signaling

Stanwick,

Fenesha,

Hamid

et al. 2024

Preprint

View full text Add to dashboard Cite

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“…Together with the troponin complex, it also controls the calcium-dependent interaction between actin and myosin during muscle contraction (12). Alternatively, spliced transcript variants encoding a variety of isoforms have been identified in smooth muscle and non-muscle cells (13). Tropomyosin 1 (TPM1) is a gene that codes for proteins.…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis of TPM1 Gene Polymorphism (rs11071720) in Patients with Non-Syndromic Cleft Lip/Palate in the South Indian Population. A Case Control Study

Murugesan,

Felicita,

Jayaseelan

2024

Odovtos - Int J Dent Sc

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The aim of this study was to find out if there was an association between TPM1 gene polymorphism (rs11071720) and non-syndromic cleft lip and palate in humans. Twenty five patients with non-syndromic cleft lip and palate (NSCL/P) and twenty five healthy patients as controls were enrolled in the study. Whole blood drawn from the participants was used to obtain genomic DNA. Sequence specific primers were used to amplify the region spanning the polymorphic site of the TPM1 gene using polymerase chain reaction (PCR). The genotype of the subjects was identified employing the RFLP technique. The genotype and allele frequency distributions in the non-syndromic cleft lip and cleft palate patients and control groups were compared using Chi-square test. In the NSCL/P, the genotype in the order from highest to lowest frequency was TC (48%), followed by CC (32%) and TT (20%). Although the genotype frequency of the case and the control groups were found to be in agreement with Hardy Weinberg Equilibrium, the genotype frequencies TC, CC and TT of the polymorphism (rs11071720) among the two groups were not statistically significant which was evident from the p-value 0.8472. No significant association was found between TPM1 gene (rs11071720) polymorphism and NSCL/P.

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Cardiovascular anomalies in patients with Tessier syndrome: a systematic review

Nazari,

Vaezi,

Mossavarali

et al. 2023

Eur J Pediatr

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The Impact of Genetic Variability of TGF-Beta Signaling Biomarkers in Major Craniofacial Syndromes

Cited by 3 publications

References 21 publications

Impaired Tertiary Dentin Secretion After Shallow Injury in Tgfbr2-Deficient Dental Pulp Cells Is Rescued by Extended CGRP Signaling

Impaired Tertiary Dentin Secretion After Shallow Injury in Tgfbr2-Deficient Dental Pulp Cells Is Rescued by Extended CGRP Signaling

Genetic Analysis of TPM1 Gene Polymorphism (rs11071720) in Patients with Non-Syndromic Cleft Lip/Palate in the South Indian Population. A Case Control Study

Cardiovascular anomalies in patients with Tessier syndrome: a systematic review

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