The Impact of False Positive COVID-19 Result

Alsheikh, Shahad H; Ibrahim, K. A.; Alfaraj, Dunya

doi:10.7759/cureus.20375

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…In a high prevalence area, the PPV will increase but the NPV will decrease indicating that in a high prevalent risk population, false negatives will become more of an issue. While in a low prevalence setting with a decreased PPV and an increased NPV, false positives will be a concern 10,11 .…”

Section: False Negative and False Positive Resultsmentioning

confidence: 99%

RT-qPCR Protocol Optimization Assessment with Propidium Monoazide for Viable and Non-Viable Pathogen Detection

Mariam Sajid,

Mohamed Askar,

Roger Bayston

2023

Proceedings S.Z.M.C

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Real time PCR or RT-qPCR (including reverse transcriptase PCR) is a gold standard in diagnostic testing for pathogens such as bacteria and viruses that contain RNA or DNA for amplification. As a validated molecular assay technique, RT- qPCR has been instrumental in microbial detection because of its high throughput, quantification of DNA or RNA samples as well as quick turnaround especially when compared with culturing methods that can take multiple days to generate result. While the advancement of PCR has had significant impact for diagnosis of diseases such as COVID- 19through its high sensitivity and specificity, the test also brings with it its own challenges of false positive and false negative results. This is normally attributed to PCR's susceptibility to contamination or inhibitors. However, there are other conditions like DNA extraction techniques and protocols in PCR methodology that can impact the accuracy of the PCR results as well. This paper, therefore, aims to synthesize factors that contribute to false positive and negatives in PCR results, improvements to protocols that includes the use of DNA intercalating dyes such as Propidium Monoazide (PMA) in PCR assay to improve DNA extraction and discuss the importance of an optimized PCR assay for diagnostic testing efficacy from a social and economic standpoint.

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Section: False Negative and False Positive Resultsmentioning

confidence: 99%

RT-qPCR Protocol Optimization Assessment with Propidium Monoazide for Viable and Non-Viable Pathogen Detection

Mariam Sajid,

Mohamed Askar,

Roger Bayston

2023

Proceedings S.Z.M.C

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“…On the contrary, there have existed several case reports that illustrate false-positive and false-negative test results but they do not provide important information, such as the brand name of the test kit that the false result had occurred or the overall preexisting medical condition of the reported case. One can find several case reports in the current literature, with such content describing false tests either regarding nucleic acid detection or antigen and antibody detection [ 55 , 56 , 57 , 58 , 59 , 60 ]. As a result, one cannot search for the specific performed test kit protocol to read the limitations of the performed test so as to identify the precise molecular reason of the false test result.…”

Section: Current Covid-19 Pandemic-derived Molecular Diagnostics’ Fac...mentioning

confidence: 99%

The Deceptive COVID-19: Lessons from Common Molecular Diagnostics and a Novel Plan for the Prevention of the Next Pandemic

Mouliou¹

2023

Diseases

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The COVID-19 pandemic took place during the years 2020–2022 and the virus, named SARS-CoV-2, seems likely to have resulted in an endemic disease. Nevertheless, widespread COVID-19 has given rise to several major molecular diagnostics’ facts and concerns that have emerged during the overall management of this disease and the subsequent pandemic. These concerns and lessons are undeniably critical for the prevention and control of future infectious agents. Furthermore, most populaces were introduced to several new public health maintenance strategies, and again, some critical events arose. The purpose of this perspective is to thoroughly analyze all these issues and the concerns, such as the molecular diagnostics’ terminologies, their role, as well as the quantity and quality issues with a molecular diagnostics’ test result. Furthermore, it is speculated that society will be more vulnerable in the future and prone to emerging infectious diseases; thus, a novel preventive medicine’s plan for the prevention and control of future (re)emerging infectious diseases is presented, so as to aid the early prevention of future epidemics and pandemics.

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“…Pengambilan sampel Purposive diadopsi dalam penelitian ini, karena subjek sesuai kriteria tujuan penelitian berasal dari populasi yang besar dan tidak diketahui, sehingga pengacakan tidak mungkin dilakukan (Etikan, 2016). Pada data sampel diidentifikasi apakah ada peningkatan melebihi jumlah Seperti dinyatakan oleh (Healy et al, 2021) dan (Alsheikh et al, 2022), hasil tes COVID-19 yang salah dapat membawa permasalahan yang lebih berat pada kondisi fisik seseorang, dan karantina atau terapi berlebihan. Hal-hal itu perlu diantisipasi untuk penafsiranhasil penelitian ini.…”

Section: Pengolahan Dataunclassified

Model Eksponensial Estimasi Efek Pembatasan Parsial (Ppkm) Dan Varian Delta Covid-19 Di Dki Jakarta

Maharesi

2023

THEOREMS

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ABSTRAKSebagai penyakit menular yang dipicu oleh Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), kode genetika virus terus berevolusi hingga sampai pada varian dominan yang diketahui adalahvtipe Delta yang memperpanjang pandemi di seluruh dunia. Dibandingkan dengan varianlain seperti Alfa, Beta dan Omicron yang lebih baru, varian Delta adalah salah satu yang paling parah menyerang sistem imun tubuh manusia, seperti yang dapat dilihat di situs Worldometer di mana jumlah kematian harian yang tinggi di banyak negara. Kondisi ini mendorong pemerintah untuk mengambil keputusan yang sangat sulit melalui sejumlah kebijakan pembatasan mobilitas penduduk yang sangat ketat. Penelitian ini dimaksudkan untuk mengukur pengaruh jumlah tes harian, pengaruh varian delta COVID-19 dan penerapan pembatasan aktivitas parsial dan mobilitas masyarakat terhadap pertumbuhan harian kasus positif di Provinsi DKI Jakarta. Dalam penelitian ini, kasus positif harian dimodelkan sebagai fungsi pertumbuhan eksponensial basis 3yang estimasi parameternya dilakukan dengan menggunakan teknik regresi linierberganda. Hasil estimasi menunjukkan adanya pengaruh signifikan dengan taraf nyata 5 % dari varian Delta berdasarkan jumlah uji COVID-19 harian terhadap jumlah kasus baru harian dengan faktor perkalian sekitar 2,6. Sedangkan pengaruh pembatasan sebagian aktivitas dan mobilitas masyarakat, secara kasar mampu secara signifikan dapat menekan hingga setengah dari potensi kasus positif harian yang mungkin terjadi.Kata kunci: virus varian delta, pembatasan aktivitas komunitas, jumlah kasus positif harian, jumlah tes harian

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The Impact of False Positive COVID-19 Result

Cited by 3 publications

References 17 publications

RT-qPCR Protocol Optimization Assessment with Propidium Monoazide for Viable and Non-Viable Pathogen Detection

RT-qPCR Protocol Optimization Assessment with Propidium Monoazide for Viable and Non-Viable Pathogen Detection

The Deceptive COVID-19: Lessons from Common Molecular Diagnostics and a Novel Plan for the Prevention of the Next Pandemic

Model Eksponensial Estimasi Efek Pembatasan Parsial (Ppkm) Dan Varian Delta Covid-19 Di Dki Jakarta

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