The impact of extraction method and pollen concentration on community composition for pollen metabarcoding
Arne Devriese,
Gerrit Peeters,
Rein Brys
et al.
Abstract:PremisePlants and pollinators closely interact with each other to form complex networks of species interactions. Metabarcoding of pollen collections has recently been proposed as an advantageous method for the construction of such networks, but the extent to which diversity and community analyses depend on the extraction method and pollen concentration used remains unclear.MethodsIn this study, we used a dilution series of two pollen mixtures (a mock community and pooled natural pollen loads from bumblebees) t… Show more
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