The impact of epididymal proteins on sperm function

Björkgren, Ida; Sipilä, Petra

doi:10.1530/rep-18-0589

Cited by 64 publications

(43 citation statements)

References 116 publications

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“…Thus, the development of spermatozoa completely depends on the function of Sertoli cells (Franca, Hess, Dufour, Hofmann, & Griswold, ). The epididymis is another important part of the reproductive system which helps in the storage and maturation of spermatozoa (Björkgren & Sipilä, ). Spermatozoa in the epididymis are prone to oxidative stress because of lack of endogenous antioxidant defence system and efficient macro‐environmental protection exerted by Sertoli cells (Kim et al, ).…”

Section: Discussionmentioning

confidence: 99%

Ameliorative effect of nerolidol on cyclophosphamide‐induced gonadal toxicity in Swiss Albino mice: Biochemical‐, histological‐ and immunohistochemical‐based evidences

et al. 2020

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Cyclophosphamide (CP) is commonly used as antineoplastic and immunosuppressant drug with noticeable gonadotoxic profile. Nerolidol (NER) is a sesquiterpene with potent antioxidant and anti‐inflammatory properties. Thus, the present study was designed to explore its possible gonadal protective potential against cyclophosphamide‐induced testicular, epididymal, seminal and spermatozoal toxicities. Animals were divided into five groups: control (normal saline for 14 days), treatment group (NER 200 and 400 mg/kg, p.o) for 14 days along with a single dose of cyclophosphamide (200 mg/kg, i.p) on 7th day, toxic and Per se groups (cyclophosphamide 200 mg/kg i.p) on 7th day and NER 400 mg/kg for 14 days respectively. Animals were sacrificed on the 15 day, and body weight, weight of reproductive organs, testosterone level, sperm count, biochemical parameters, histopathological and immunohistochemical studies were performed in the testes, epididymis and in the serum. CP administration induced oxidative stress, nitrative stress, inflammation, reduced testosterone level, sperm count, increased expression of MPO and caused histological aberrations in the testes, epididymis and seminal vesicles. CP caused reduced sperm count, sperm motility and testosterone level which got reversed upon treatment with nerolidol in a dose‐dependent manner. Nerolidol thus acted as a gonadoprotective molecule and prevented the gonadotoxicity of CP.

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Section: Discussionmentioning

confidence: 99%

Ameliorative effect of nerolidol on cyclophosphamide‐induced gonadal toxicity in Swiss Albino mice: Biochemical‐, histological‐ and immunohistochemical‐based evidences

et al. 2020

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“…Starting from the 1960s important sperm functions, such as the acquisition of sperm progressive motility and fertilizing capacity, were hypothesized under the control of the epididymis [ 56 , 57 ]. Considering that it is not surprising that epididymosomes convey to SPZ a large cargo of proteins encompassing key classes of enzymes, chaperones and structural proteins; for example, the cysteine-rich secretory protein 1 (CRISP1) regulates Ca 2+ channels in the sperm membrane [ 58 ], the sperm adhesion molecule 1 (SPAM1, also known as PH-20) is involved in sperm-zona pellucida adhesion [ 59 ] and the macrophage migration inhibitory factor (MIF) actively participates in sperm motility acquisition [ 60 ].…”

Section: Exosomes In the Male Reproductive Tract: Their Potential mentioning

confidence: 99%

“…Along the epididymis, sperm proteins undergo several post-translational modifications, despite SPZ being silent cells [ 57 ]. This is the case of two proteins, both required for gamete interaction: IZUMO1—whose cytoplasmic domain becomes heavily phosphorylated in the epididymis—and ADAM3 (also known as cyritestin).…”

Section: Exosomes In the Male Reproductive Tract: Their Potential mentioning

confidence: 99%

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

Candenas

Chianese

2020

IJMS

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Infertility has become a global health issue, with approximately 50% of infertility cases generated by disorders in male reproduction. Spermatozoa are conveyed towards female genital tracts in a safe surrounding provided by the seminal plasma. Interestingly, this dynamically changing medium is a rich source of proteins, essential not only for sperm transport, but also for its protection and maturation. Most of the seminal proteins are acquired by spermatozoa in transit through exosomes (epididymosomes and prostasomes). The high number of seminal proteins, the increasing knowledge of their origins and biological functions and their differential expression in the case of azoospermia, asthenozoospermia, oligozoospermia and teratozoospermia or other conditions of male infertility have allowed the identification of a wide variety of biomarker candidates and their involvement in biological pathways, thus to strongly suggest that the proteomic landscape of seminal plasma may be a potential indicator of sperm dysfunction. This review summarizes the current knowledge in seminal plasma proteomics and its potentiality as a diagnostic tool in different degrees of male infertility.

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“…Four cell populations (C0: Principle Cell; C3: Clear/Narrow Cells; C4: Basal Cells; C5: Sperm cells) were distally reduced, while two populations (C1: Novel epididymal cell identified in this study, C2: Halo/T Cells) were distally increased (Figure 2C). Intriguingly, expressions of lots genes showed segment-specific or distally changed pattern (Bjorkgren and Sipila, 2019; Sipila and Bjorkgren, 2016), suggesting their expression patterns might be altered along with the cell number changes. The spatial distribution changes of each epididymal cell population might help to reveal their functionalities.…”

Section: Resultsmentioning

confidence: 99%

“…Epididymal cells secret plenty of the proteins, small RNA and extracellular vesicles into the epididymal lumen to regulate sperm maturation (Bjorkgren and Sipila, 2019). DNA binding transcription factors (TFs) play center roles in the regulation of gene expression.…”

Section: Discussionmentioning

confidence: 99%

The landscape of mouse epididymal cells defined by the single-cell RNA-Seq

Shi

Sang

Xie

et al. 2020

Preprint

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Spermatozoa acquire their fertilizing ability and forward motility properties during epididymal transit. Although lots of attempts elucidating the functions of different cell types in epididymis, the composition of epididymal tubal and cell types are still largely unknown. Using single-cell RNA sequence, we analyzed the cell constitutions and their gene expression profiles of adult epididymis derived from caput, corpus and cauda epididymis with a total of 12,597 cells. This allowed us to elucidate the full range of gene expression changes during epididymis and derive region-specific gene expression signatures along the epididymis. A total of 7 cell populations were identified with all known constituent cells of mouse epididymis, as well as two novel cell types. Our analyses revealed a segment to segment variation of the same cell type in the three different part of epididymis and generated a reference dataset of epididymal cell gene expression. Focused analyses uncovered nine subtypes of principal cell. Two subtypes of principal cell, c0.3 and c.6 respectively, in our results supported with previous finding that they mainly located in the caput of mouse epididymis and play important roles during sperm maturation. We also showed unique gene expression signatures of each cell population and key pathways that may concert epididymal epithelial cell-sperm interactions. Overall, our single-cell RNA seq datasets of epididymis provide a comprehensive potential cell types and information-rich resource for the studies of epididymal composition, epididymal microenvironment regulation by the specific cell type, or contraceptive development, as well as a gene expression roadmap to be emulated in efforts to achieve sperm maturation regulation in the epididymis.

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The impact of epididymal proteins on sperm function

Cited by 64 publications

References 116 publications

Ameliorative effect of nerolidol on cyclophosphamide‐induced gonadal toxicity in Swiss Albino mice: Biochemical‐, histological‐ and immunohistochemical‐based evidences

Ameliorative effect of nerolidol on cyclophosphamide‐induced gonadal toxicity in Swiss Albino mice: Biochemical‐, histological‐ and immunohistochemical‐based evidences

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

The landscape of mouse epididymal cells defined by the single-cell RNA-Seq

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