The impact of electrospun films of poly(ε-caprolactone) filled with nanostructured zeolite and silica microparticles on in vitro histamine formation by Staphylococcus aureus and Salmonella Paratyphi A

Abstract

This study originally reports the preparation and characterization of electrospun films based on poly(ε-caprolactone) (PCL) with high histamine-binding capacity. To this end, submicron PCL fibers filled with nanostructured zeolite or silica (SiO2) microparticles in the 5-20 wt% range were first prepared by electrospinning. The resultant electrospun composite fiber mats were thereafter thermally post-treated at 55 °C to successfully develop contact-transparent films with reduced porosity and improved mechanical strength. The capacity of the developed composite films to entrap histamine was evaluated in vitro by the culture media method using Staphylococcus aureus (S. aureus) and Salmonella Paratyphi A (S. Paratyphi A) food-borne bacteria. Both electrospun zeoliteand SiO2-containing PCL films exhibited high histamine-binding capacity, being more effective for S. aureus. The histamine entrapment performance was significantly higher for the PCL films filled with zeolite due to the enhanced porous structure and more optimal adsorption selectivity of this mineral. The here-developed electrospun composite films can be originally applied as novel activescavenging packaging materials to entrap heat-stable histamine and other biogenic amines released from fish and fishery products.

Development of Electrospun Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Monolayers Containing Eugenol and Their Application in Multilayer Antimicrobial Food Packaging

“…In another study, compression-molded bilayer structures based on PHBV containing different essential oils at 15 wt% were produced by Requena et al (91), showing that the E and σ y values ranged between 1,141-773 MPa and 27.6-17 MPa, respectively. These results confirm that the electrospun film show lower mechanical strength due to they consist of coalesced fibers and also show higher porosity (92). In any case, all the developed monolayers showed sufficient strength to be successfully applied as interlayers in packaging applications.…”

“…In a context of packaging applications for food preservation, the present PHAs showed values of permeability relatively close to petroleum derived thermoplastics such as polyethylene terephthalate (PET), with WVP value around 5.2 × 10 −15 Kg·m·m −2 ·s −1 ·Pa −1 ( 54 , 87 ). As described during the mechanical analysis, the differences attained can be ascribed to the remaining porosity and, thus, lower continuity of the annealed fiber-based material ( 92 ). One can also observe that the incorporation of eugenol into the PHBV monolayers decreased the permeability of both vapors whereas there were also significant differences for the electrospun monolayers containing eugenol.…”

“…The WVP and LP values of the electrospun PHBV monolayers containing eugenol, food contact PHBV film, and structural PHB sheet are shown in (54,87). As described during the mechanical analysis, the differences attained can be ascribed to the remaining porosity and, thus, lower continuity of the annealed fiber-based material (92). One can also observe that the incorporation of eugenol into the PHBV monolayers decreased the permeability of both vapors whereas there were also significant differences for the electrospun monolayers containing eugenol.…”

Electrospinning of PCL-Based Blends: Processing Optimization for Their Scalable Production

“…Polymeric electrospun mats are very versatile materials and they can be used for the production of monolayer and multilayer films and/or sandwich type composites [ 6 , 7 , 8 , 9 ]. Moreover, the electrospun mats can be easily modified by reinforcing the fibers with organic [ 10 , 11 ] and inorganic nanofillers [ 12 , 13 , 14 ] and metallic nanoparticles [ 15 , 16 ]. Therefore, the electrospun mats have gained interest in the industrial sector since they can be easily processed as multifunctional materials and can be applied in different industrial fields such as controlled release of drugs [ 13 ], antioxidants [ 10 , 17 ] and/or antimicrobial [ 17 ] agents; in addition they have piezoelectric properties [ 18 ] and shape memory performance [ 19 , 20 ].…”

Preparation and Applications of Electrospun Optically Transparent Fibrous Membrane

“…A nanofibrous membrane with antibacterial property is also a major development direction of food packaging materials. Food poisoning is usually caused by histamine produced by food-borne bacteria such as Staphylococcus aureus, Proteus and Salmonella paratyphi A. Erbay et al [ 163 ] prepared a submicron PCL nanofibrous membrane filled with nano zeolite and silica (SiO 2 ) by electrospinning, and then heat-treated it to obtain a transparent fibrous membrane with significantly improved mechanical properties, which effectively captured histamine produced by Staphylococcus aureus and paratyphoid bacillus.…”