The impact of electromagnetic fields with frequency of 50 Hz on metabolic activity of cells in vitro

Koziorowska, Anna; Sołek, Przemysław; Majchrowicz, Lena; Romerowicz-Misielak, Maria

doi:10.15199/48.2017.01.39

Cited by 3 publications

(4 citation statements)

References 23 publications

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“…The amount of heat depends on the characteristics of the applied field and the nature and size of the nanoparticles [37]. Many in vitro and in vivo studies, which examined the process, have confirmed the hypothesis [30][31][32]. The studies have shown that heat and EMF have a strong synergis-tic impact on the death of tumor cells [34].…”

Section: Discussionmentioning

confidence: 99%

“…For example, it was reported that EMF can increase the concentration of free radicals through the Fenton reaction , which damaged biomolecules such as the DNA and induced cell death [28,29]. In fenton reaction hydroxyl radical is produced from hydrogen peroxide in the presence of ferrous iron: Another study showed that EMF at a 2-mT intensity and 50 Hz frequency increased intracellular cAMP and inhibited cell proliferation [30]. Another report showed that a 50-Hz EMF reduced the antioxidant defense system significantly in the mouse brain [31].…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Synthesized Superparamagnetic Iron Oxide Nanoparticles and Electromagnetic Field on Cell Death of MCF-7 Breast Cancer Cell Line

Satari

Haghighat

Jouni

et al. 2018

Multidiscip Cancer Investig

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The reduction in the size of iron oxide nanoparticles causes an increase in their surface-to-volume ratio. The large surface area of magnetic nanoparticles dramatically changes their magnetic properties. Iron oxide nanoparticles smaller than 30 nm can be considered to have a single magnetic domain. They exhibit superparamagnetic properties and are hence called superparamagnetic iron oxide nanoparticles (SPIONs). SPIONs exhibit extraordinary magnetic properties in the presence of an external magnetic field; they quickly reach magnetic saturation and do not retain any magnetism after the external magnetic field has been removed. This property prevents them from accumulating in the capillaries and causing blockage [1]. The attractive properties of SPIONs such as super-paramagnetic, high-field irreversibility, and high saturation field have made them ideal candidates for biomedical applications such as contrast agents for magnetic resonance imaging (MRI), targeted drug delivery in tumor therapy [2,3] and hyperthermia [4], controlled release of drugs [5], and targeting gene delivery or magnetofection [6,7]. Cancer is a group of diseases characterized by uncontrolled cell growth and the possibility of metastasis [8]. Breast cancer has the highest mortality after lung cancer worldwide and is the most common-Abstract Introduction: Iron oxide nanoparticles, owing to their very small size and superparamagnetic properties, have been considered a potential candidate for several medical applications such as magnetic cell separation, magnetic resonance imaging (MRI), magnetic targeted drug delivery magnetic hyperthermia. The present study aimed to synthesize and evaluate the characteristics of superparamagnetic iron oxide nanoparticles (SPIONs) and determine the mechanism by which they induce cell death in the presence of an extremely low-frequency magnetic field (ELMF). Methods: First, SPIONs were synthesized using the chemical co-precipitation method and then characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential. A vibrating-sample magnetometer (VSM) was used to measure the magnetic properties of the nanoparticles. Human MCF-7 breast cancer cells were treated with different concentrations of SPION in the absence and presence of a 50-Hz ELMF for 24 and 48 h. Cytotoxicity and cell viability percentage in the treated cells were measured by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Results: DLS and TEM analyses indicated that the SPIONs have an average size of less than 30 nm and they are superparamagnetic. VSM analyses also confirmed the superparamagnetic nature of the nanoparticles. The MTT assay indicated that high concentrations of SPIONs induced death in MCF-7 cells. In the groups treated with ELMF+SPIONs, cell death increased sharply compared with that in the groups treated with each treatment alone (P≤0.05). Conclusions: It seems that a 50-Hz ELMF in the presence of SPIONs led to cell death due to local heating

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Effects of Synthesized Superparamagnetic Iron Oxide Nanoparticles and Electromagnetic Field on Cell Death of MCF-7 Breast Cancer Cell Line

Satari

Haghighat

Jouni

et al. 2018

Multidiscip Cancer Investig

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“…Sun et al have investigated that human promyelocytic leukaemia cells exposed to a 900 MHz radiofrequency field would damage mitochondrial DNA by generating reactive oxygen species pathway [7]. Koziorowska et al report a significantly different viability decrease in the cancer line compared with the increase of viability of the normal cells at different EMF exposure times [8]. The influence of EMF on cellular mechanisms is observed both inside and outside the cell.…”

Section: Introductionmentioning

confidence: 99%

Power loss model of electromagnetic waves propagation in cell solutions by GHz electrical impedance spectroscopy

Zhang,

Liu,

Wang

et al. 2024

Meas. Sci. Technol.

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The propagation of GHz electromagnetic waves across cells in cell solutions has been analytically modelled and numerically calculated in order to elucidate the power loss in the boundary between dispersed medium and cell by establishing a theoretical model. Living and dead yeast cells are chosen as objects because of the simple cell structure and ease of observation under optical microscope. Through the model, the average power density of the incident wave Savi, reflected wave Savr, transmitted wave Savt, and ratio of the power loss ψ are calculated and compared to analyse the power loss of electromagnetic waves inside living and dead yeast cells by considering the impacts of frequency of electromagnetic wave, cell viability, concentration, and component structures of the cell. Results demonstrate decreased Savi, Savr, and Savt with rising frequency, especially noticeable below 100MHz due to enhanced absorption from cell components. Electromagnetic waves in living yeast cell solutions exhibit faster attenuation and stronger reflection compared to dead yeast cells, attributed to intact organelles and membranes intensifying absorption and scattering. The increasing cells concentration further attenuates electromagnetic waves. This work elucidates propagation and power loss of electromagnetic waves in cell solutions and provides an effective computational approach to optimize electromagnetic wave based biomedical applications.

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“…The reagent stains dead cells in blue, but does not penetrate into viable cells, so they remain unstained because the cell membrane remains a barrier. This method has been described in the paper [3].…”

mentioning

confidence: 99%

Biological effects of the EMF influence on animal cells and tissues in in vitro cultures - a summary of own research

Koziorowska

2018

ELECTROTECHNICAL REVIEW

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The effects of the electromagnetic field on cells and tissue in in vitro cultures are the subject of the research presented in this work.. Choosing different research object-cancerous and non-cancerous cells, male and female reproductive tissues, and various physical parameters of the EMF, such as the frequency of time-varying fields, magnetic induction value, field shape and time of exposure, there were tested effects (negative or positive) of impact, measured by modern methods of molecular biology through biological parameters in in vitro cultures. Streszczenie. Pole elektromagnetyczne, jako czynnik środowiskowy wpływający na komórki i tkanki hodowane in vitro jest tematem badań przedstawionych w niniejszej pracy. Wybierając różne obiekty badawcze-komórki nowotworowe i nienowotworowe, tkanki męskiego i żeńskiego układu rozrodczego oraz różne parametry fizyczne pola elektromagnetycznego, takie jak częstotliwość pól zmiennych w czasie, wartość indukcji magnetycznej, kształt pola i czas ekspozycji, badano efekty (negatywne lub pozytywne) oddziaływania pola, mierzone nowoczesnymi metodami biologii molekularnej w kulturach in vitro. (Biologiczne efekty oddziaływania PEM jako czynnika środowiskowego na komórki i tkanki zwierzęce w hodowlach in vitro-podsumowanie badań własnych)

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The impact of electromagnetic fields with frequency of 50 Hz on metabolic activity of cells in vitro

Cited by 3 publications

References 23 publications

The Effects of Synthesized Superparamagnetic Iron Oxide Nanoparticles and Electromagnetic Field on Cell Death of MCF-7 Breast Cancer Cell Line

The Effects of Synthesized Superparamagnetic Iron Oxide Nanoparticles and Electromagnetic Field on Cell Death of MCF-7 Breast Cancer Cell Line

Power loss model of electromagnetic waves propagation in cell solutions by GHz electrical impedance spectroscopy

Biological effects of the EMF influence on animal cells and tissues in in vitro cultures - a summary of own research

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