The Impact of Egg Ozonation on Hatching Success, Larval Growth, and Survival of Atlantic Cod, Atlantic Salmon, and Rainbow Trout

Fry, Jessica S.; Casanova, Juan Pérez; Hamoutene, Dounia; Lush, L.; Walsh, Andy; Couturier, C.

doi:10.1080/08997659.2014.983278

Cited by 6 publications

(4 citation statements)

References 35 publications

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“…The degree of ozone penetration across egg shells was not correlated with gas concentration in the environment. This is in agreement with previous reports indicating that there are a high number of pores on the egg shell blunt end just above the air cell (Fry et al 2015). Braun et al (2011), have investigated the feasibility of gaseous ozone to reduce the number of microorganisms on the shell surface, of Salmonella enteritidis (S.e) in particular, of avian hatching eggs.…”

Section: Resultssupporting

confidence: 91%

Ozone treatment as a disinfectant of commercial eggs to preserve function quality

Parvin

Zakeri

Hidarnejad

et al. 2020

Indian J of Anim Sci

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Ozone is a strong oxidant used for the disinfection of surfaces, drinking water and foods. However, since ozone not only destroys bacteria but may also damage eggs, it is necessary to clarify the effects of ozone treatment on commercial egg components. In this study, different doses of ozone gas, 2, 4, 6 and 10 ppm were used. Salmonella entritidis infected eggs were dosed in different experimental groups at different doses of ozone for 15 min. Factors such as TBC, Salmonella entritidis, pH of egg contents, shell hardness, peroxide content in egg contents and albumen viscosity were measured after 10 days of application. The best amount of ozone gas was obtained at concentrations of 6 and 10 ppm for disinfection of different parts of the egg. While the amount of peroxide produced at 10 ppm was more than standard and harmful. Generally, the application of ozone gas at doses of 4 ppm, especially at 6 ppm, showed the best results for the measured factors. The use of gas at 6 ppm for 15 min can be used to keep the eggs fresh with maximum disinfection. This can be done in the egg industry, in order to remove Salmonella entritidis and maintain the quality of commercial eggs.

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Section: Resultssupporting

confidence: 91%

Ozone treatment as a disinfectant of commercial eggs to preserve function quality

Parvin

Zakeri

Hidarnejad

et al. 2020

Indian J of Anim Sci

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“…Such delays or decreases have been reported as side effects of ozonation in some marine fish species, such as Latris lineata (BATTAGLENE;MOREHEAD 2006). On the other hand, as demonstrated in zebrafish here, Oncorhynchus mykiss and Hippoglossus hippoglossus embryos have shown satisfactory hatching and growth results in ozonated water (FRY et al, 2015).…”

Section: Discussionsupporting

confidence: 68%

“…Hao et al (2015) determined that the safe concentration of ozonated water for zebrafish embryos was 0.1 mg/L and reported that this concentration allowed survival and did not harm the animals. Resistance to ozonation was also found by other authors in embryos of marine fish such as Gadus morhua and Hippoglossus hippoglossus, being 3 mg/L and 2 mg/L respectively (FRY et al, 2015;GROTMOL et al, 2003). The embryos of the marine fish species Hippoglossus hippoglossus showed greater sensitivity to ozonation (GROTMOL et al, 2003).…”

Section: Discussionsupporting

confidence: 61%

Use of ozone in the culture medium of zebrafish (Danio rerio) embryos

Virote

Martimiano

Fabem

et al. 2022

AVB

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In this study, we sought to demonstrate the toxicity of different concentrations of ozonated water in zebrafish embryos up to 120 hours postfertilization (hpf ) and their antimicrobial activity advantages. For the test, we placed 40 embryos per treatment in Petri dishes containing 30 ml of solution to be tested. The ozone concentrations used were calculated from an initial concentration of 72 μg/ml using the O&L 1.5 RM ozone generator (Ozone & Life, Brazil). Five serial dilutions 1:1 was performed in egg water to produce the ozone treatments. We also analyzed a treatment with 30 ml of egg water without ozone, with only the addition of methylene blue, and a treatment with 30 ml of egg water without the addition of any antifungal agent. The plates were incubated at 28 ± 1°C, and the embryos were analyzed daily until 120 hpf. The survival rate, incubation period, and possible deformities were analyzed. In addition, egg water microbiological analyses were performed to detect total coliforms and fungi and yeasts. The data were tested for normality, and analysis of variance was performed within Minitab®version 1.8 software. The results showed that there was no significant difference in embryo survival or hatching rate between treatments with different ozone concentrations (p > 0.05), and no embryonic deformities were found under any of the ozone concentrations evaluated. It can be concluded that ozone within the concentrations analyzed is not toxic to zebrafish embryos and has antifungal and antimicrobial action.

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“…In Fry et al. (), four treatments (ozone at low, standard and high concentrations, and Perosan) exhibited no effect on the yolk sac conversion efficiency of rainbow trout Oncorhynchus mykiss larvae of DAH 0–30. As discussed above, these effects are usually species, disinfection agent and concentration specific.…”

Section: Discussionmentioning

confidence: 96%

Phenotypic effects of antibiotic-induced axenity and egg disinfection in early larval European seabass (Dicentrarchus labraxL.)

et al. 2018

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In the present study, the hypothesis that the difference in axenic conditions in the incubation and rearing environment of European seabass larvae induces size and shape effects on the specimens is tested. This difference is studied between xenic and axenic seabass larvae of DAH (day after hatching) 0, 5, 11 and 15. The axenic rearing protocol involves an egg disinfection with glutaraldehyde after the primary one with iodine in the hatchery, and the hypothesis that this secondary disinfection induces size and shape effects is also tested. In order to accomplish this, three egg and larvae treatments are included: “DA” (disinfected axenic), “DX” (disinfected xenic) and “NX” (nondisinfected xenic). Regarding the effect of antibiotic‐induced axenity, DA larvae exhibited larger bodies than both DX and NX on DAH 5 and 11. They also had a smaller yolk sac than DX at hatching, but consumed it slower. Towards the end of the experiment, DA larvae were thicker, but slightly more curved than DX and NX, which may be an abnormal shape, or a slightly more advanced ontogenetic stage. As far as egg disinfection, it had significant but very moderate shape effects on DAH 5 and 11, and disinfected larvae consumed their yolk sac faster. To the best of our knowledge, this study is one of the first to illustrate the subtle but significant size and shape effects caused by antibiotic‐induced axenity and secondary egg disinfection in the early larval stages, which suggest the existence of bacterial mechanisms that play a phenotypic role.

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The Impact of Egg Ozonation on Hatching Success, Larval Growth, and Survival of Atlantic Cod, Atlantic Salmon, and Rainbow Trout

Cited by 6 publications

References 35 publications

Ozone treatment as a disinfectant of commercial eggs to preserve function quality

Ozone treatment as a disinfectant of commercial eggs to preserve function quality

Use of ozone in the culture medium of zebrafish (Danio rerio) embryos

Phenotypic effects of antibiotic-induced axenity and egg disinfection in early larval European seabass (Dicentrarchus labraxL.)

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