The impact of EGFR stimulation and inhibition on BPDE induced DNA fragmentation in oral/oropharyngeal mucosa in vitro

Baumeister, Philipp; Heinrich, Katharina; Märte, Melanie; Reiter, M.; Schwenk‐Zieger, Sabina; Harréus, Ulrich

doi:10.1016/j.oraloncology.2011.08.006

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…(70,71) Contrarily other studies, found no correlation between EGFR expression and tumor behavior when anti-EGFR extracellular domain antibodies. (72)(73)(74) There was no difference in the area percentage of EGFR across different degrees of OSCC differentiation in our investigation, which is consistent with Ramu et al (2018). (75) This finding may imply that the level of differentiation of malignant keratinocytes is unrelated to EGFR.…”

Section: Discussionsupporting

confidence: 92%

Therapeutic efficacy of time-dependent cetuximab on experimentally induced hamster buccal pouch carcinoma

Shamia

Abd-Alhafez

Alqalshy

et al. 2022

ijhs

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Purpose: This study aimed to determine the cetuximab therapeutic potential in the time-dependent fashion in experimentally induced hamster buccal pouch (HBP) carcinoma. Material and methods: Forty Syrian male hamsters were classified into four equal groups (G) of ten each. GI: The animals remained untreated to act as negative controls. The right pouches of animals in GII, GIII, and GIV were painted three times a week for 14 weeks (w)s with 7,12-dimethylbenz (a) anthracene (DMBA). GII: No additional treatment was administered. While, the animals in GIII and GIV were treated differently. Those in GIII received cetuximab intraperitoneally (IP) three intervals a week for three (w)s, whereas those in GIV received cetuximab IP three intervals for six (w)s. After the end of the experiment, the gross observations were made, and blood samples (2ml) were withdrawn from the inner canthus of the eye for analysis of whole white blood cells (WBCs) and oxidative stress markers [glutathione (GSH) and malondialdehyde (MDA) levels]. All pouches were surgically bisected for preparation for Hematoxylin and Eosin (H&E) stain and immunohistochemistry (IHC) stain using epidermal growth factor receptor (EGFR). Other fresh tissue was used for DNA detection through a flow cytometry (FCM) test.

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Section: Discussionsupporting

confidence: 92%

Therapeutic efficacy of time-dependent cetuximab on experimentally induced hamster buccal pouch carcinoma

Shamia

Abd-Alhafez

Alqalshy

et al. 2022

ijhs

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“…This effect was totally abrogated when the receptor was blocked in advance [61, 62]. Thus, in premalignant mucosa of head and neck cancer patients, EGFR stimulation protects the cell from B(a)p, most probably as a result of EGFR downstream activation of a multidrug resistance efflux pump [63] capable of extruding the carcinogen from the cell [64, 65].…”

Section: Methodological Considerationsmentioning

confidence: 99%

Curcumin and Other Polyphenolic Compounds in Head and Neck Cancer Chemoprevention

Baumeister

Reiter

Harréus

2012

Oxidative Medicine and Cellular Longevity

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Despite clear results of observational studies linking a diet rich in fruits and vegetables to a decreased cancer risk, large interventional trials evaluating the impact of dietary micronutrient supplementation, mostly vitamins, could not show any beneficial effects. Today it has become clear that a single micronutrient, given in supernutritional doses, cannot match cancer preventive effects of whole fruits and vegetables. In this regard polyphenols came into focus, not only because of their antioxidant potential but also because of their ability to interact with molecular targets within the cells. Because polyphenols occur in many foods and beverages in high concentration and evidence for their anticancer activity is best for tissues they can come into direct contact with, field cancerization predestines upper aerodigestive tract epithelium for cancer chemoprevention by polyphenols. In this paper, we summarize cancer chemopreventive attempts with emphasis on head and neck carcinogenesis and discuss some methodological issues. We present data regarding antimutagenic effects of curcumin and epigallocatechin-3-gallate in human oropharyngeal mucosa cultures exposed to cigarette smoke condensate.

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“…These cells were exposed to tobacco carcinogen to produce DNA damage and enhanced EGFR expression. Inhibition of EGFR also suppressed DNA damage [64]. In addition one study of experimental oral carcinogenesis showed inhibition of EGFR also suppressed DNA damage [65].…”

Section: Discussionmentioning

confidence: 97%

Inhibition of EGFR Suppresses Ethyl Alcohol and Tobacco Cell Effects on Growth of Human Oral Keratinocytes and Human Papillomavirus 16 Entry as a Function of Furin

Schwartz¹,

Munaretto²,

Bagchi³

et al. 2015

JCT

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Background: Reported are increased risks for malignant transformation in human oral keratinocytes (HOK) from ethyl alcohol (ETOH), tobacco products or human papilloma virus oncogenic subtype 16 (HPV 16) infections. We examined various HOK cell responses to these factors to show inhibitors of epidermal growth factor receptor (EGFR) also inhibits furin; proprotein convertase (FC) and HPV 16 entry in HOK. Methods: Immortalized HOK by HPV 16 (HPV 16B) or human telomerase (hTERT); primary foreskin keratinocytes (NHFK), primary HOK, buccal keratinocytes (NHBK) and oral SCC-25 were treated with dibenz[a,l]pyrene (DBP), anthraquinone; nitrosamine (NNAL) or ethyl alcohol (ETOH) and acetaldehyde (AA). ETOH was tested for synthesis of malondialdehyde (MDA) and alcohol dehydrogenase expression (ADH). ETOH, and PAH were evaluated by Western immunoblot for oncogene changes, and phosphorylated EGFR expression. Inhibition of EGFR by WZ4002 and Erlotinib and/or carcinogens effect on HPV 16 entry were studied. A green fluorescent pseudovirus (PsV); chloromethylketone (CMK) an inhibitor of furin activity and Western immunoblot of furin cell distribution further characterized HPV 16 entry. Results: ETOH (10 μM) increased expression of phosphorylated EGFR and HPV 16 entry through furin activity, and membrane, nuclear and cytoskeletal accumulations. CMK suppressed HPV 16 entry and blockage of ADH while aldehyde dehydrogenase (ALDH) enhanced HPV 16 entry. Similarly PAH, DBP (4-8 nM), anthraquinone (98 nM) and NNAL (6.9 μM) enhanced HPV 16 entry through furin activity and mem-J. L. Schwartz et al. 91 brane, nuclear and cytoskeletal accumulations. Furthermore, WZ4002 and Erlotinib suppressed expressions of phosphorylated EGFR, FC activity, and HPV 16 entry. ETOH and DBP treatments also enhanced expressions of protease activated receptor-1 (PAR-1), and p21 waf1 while depressed p16 and p27 KIP1 expressions in HOK/HPV 16B cells. Conclusion: EGFR inhibitors are candidates for suppression of alcohol and tobacco effects on EGFR phosphorylated expression; keratinocyte growth, and HPV 16 entry and prevention treatment for HPV related diseases.

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The impact of EGFR stimulation and inhibition on BPDE induced DNA fragmentation in oral/oropharyngeal mucosa in vitro

Cited by 5 publications

References 32 publications

Therapeutic efficacy of time-dependent cetuximab on experimentally induced hamster buccal pouch carcinoma

Therapeutic efficacy of time-dependent cetuximab on experimentally induced hamster buccal pouch carcinoma

Curcumin and Other Polyphenolic Compounds in Head and Neck Cancer Chemoprevention

Inhibition of EGFR Suppresses Ethyl Alcohol and Tobacco Cell Effects on Growth of Human Oral Keratinocytes and Human Papillomavirus 16 Entry as a Function of Furin

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