The impact of early-life stress on the expression of HPA-associated genes in the adult murine brain

Reshetnikov, Vasiliy V.; Studenikina, A. A.; Ryabushkina, Julia A.; Merkulova, T. I.; Bondar, Natalia P.

doi:10.1163/1568539x-00003482

Cited by 15 publications

(16 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nonetheless, in our experiment, the higher Nr1d1 expression did not result in alteration of Nr3c1 or Nr3c2 expression in the prefrontal cortex and hippocampus of adult female mice. Similarly, in our recent study [61] on males, we did not detect changes in the expression of either Nr3c1 or Nr3c2 under the influence of early-life stress, in agreement with the absence of such changes in females, but prolonged maternal separation resulted in a higher Nr3c2/Nr3c1 mRNA ratio in the 6 Behavioural Neurology hippocampus and hypothalamus in males. Thus, we did not detect an influence of early-life stress on Nr3c1 or Nr3c2 expression in females, at least under unstressful conditions.…”

Section: Early-life Stress Increases the Expression Of Nr1d1 But Doessupporting

confidence: 91%

Maternal Separation Early in Life Alters the Expression of Genes Npas4 and Nr1d1 in Adult Female Mice: Correlation with Social Behavior

Ryabushkina

Reshetnikov

Bondar

2020

Behavioural Neurology

View full text Add to dashboard Cite

Early-life stress affects neuronal plasticity of the brain regions participating in the implementation of social behavior. Our previous studies have shown that brief and prolonged separation of pups from their mothers leads to enhanced social behavior in adult female mice. The goal of the present study was to characterize the expression of genes (which are engaged in synaptic plasticity) Egr1, Npas4, Arc, and Homer1 in the prefrontal cortex and dorsal hippocampus of adult female mice with a history of early-life stress. In addition, we evaluated the expression of stress-related genes: glucocorticoid and mineralocorticoid receptors (Nr3c1 and Nr3c2) and Nr1d1, which encodes a transcription factor (also known as REVERBα) modulating sociability and anxiety-related behavior. C57Bl/6 mice were exposed to either maternal separation (MS, 3 h once a day) or handling (HD, 15 min once a day) on postnatal days 2 through 14. In adulthood, the behavior of female mice was analyzed by some behavioral tests, and on the day after the testing of social behavior, we measured the gene expression. We found increased Npas4 expression only in the prefrontal cortex and higher Nr1d1 expression in both the prefrontal cortex and dorsal hippocampus of adult female mice with a history of MS. The expression of the studied genes did not change in HD female mice. The expression of stress-related genes Nr3c1 and Nr3c2 was unaltered in both groups. We propose that the upregulation of Npas4 and Nr1d1 in females with a history of early-life stress and the corresponding enhancement of social behavior may be regarded as an adaptation mechanism reversing possible aberrations caused by early-life stress.

show abstract

Section: Early-life Stress Increases the Expression Of Nr1d1 But Doessupporting

confidence: 91%

Maternal Separation Early in Life Alters the Expression of Genes Npas4 and Nr1d1 in Adult Female Mice: Correlation with Social Behavior

Ryabushkina

Reshetnikov

Bondar

2020

Behavioural Neurology

View full text Add to dashboard Cite

show abstract

“…The MS is a stress‐causative factor that has been used to create mental health disturbances in different studies, particularly studies on stress and reproductive system disorders . Furthermore, It was demonstrated that the implications from early life stress appear later in the adulthood …”

Section: Discussionmentioning

confidence: 99%

Implications from early life stress on the development of mouse ovarian follicles: Focus on oxidative stress

Ghatebi

Zavareh

Lashkarbolouki

et al. 2019

J of Obstet and Gynaecol

View full text Add to dashboard Cite

Aim: The early life stress has significant long-term effects on the development of the offspring. This study was undertaken to verify if maternal separation as a stressor agent affects the oxidative status and developmental competence of mouse pre-antral follicles (PF) during in vitro culture period. Methods: Female litters of National Medical Research Institute mice were divided into two groups: maternally separated group (MS), separated from the mothers for 6 h per day from postnatal days 2-16; and the rest considered as the control group, which left undisturbed over the 14 days. The litters were sacrificed and the ovarian tissue was harvested to isolate the PF. The PF were in vitro cultured up to 12th day when ovulation was induced. The developmental parameters and oxidative status (i.e., total antioxidant capacity and Malondialdehyde levels, as well as the activities of superoxide dismutase, glutathione peroxidase and catalase) were assessed. Results: The rates of survival, antrum formation, ovulation and oocyte maturation of PF derived from the MS group were significantly lower compared with those of the control group. Furthermore, the Malondialdehyde level of the MS group was significantly higher than that of the control group. By contrast, the total antioxidant capacity level was lower in the MS group with respect to the control group. Also, the activity of superoxide dismutase, glutathione peroxidase and catalase of PF, derived from the MS group, was significantly lower compared with those of the control group. Conclusion: Early life stress damages the developmental competence of mouse PF through induction of oxidative stress.The developmental competence of PF was assessed by measuring the follicle diameter on the 2nd and 4th day of the cultivation period, antrum formation, 1507

show abstract

“…Many studies show the critical importance of the postnatal period for individual development because stress in this period can have delayed effects on cognition, individual behavior, neuronal plasticity and epigenetics . Nevertheless, the mouse and rat studies on MS and HD effects on cognition in adult females are limited.…”

Section: Discussionmentioning

confidence: 99%

Stress early in life leads to cognitive impairments, reduced numbers of CA3 neurons and altered maternal behavior in adult female mice

Reshetnikov

Ковнер

Lepeshko

et al. 2018

Genes Brain and Behavior

View full text Add to dashboard Cite

The hippocampus is a crucial part of the limbic system involved both in cognitive processing and in the regulation of responses to stress. Adverse experiences early in life can disrupt hippocampal development and lead to impairment of the hypothalamic‐pituitary‐adrenal axis response to subsequent stressors. In our study, two types of early‐life stress were used: prolonged separation of pups from their mothers (for 3 hours/day, maternal separation, MS) and brief separation (for 15 minutes/day, handling, HD). In the first part of our study, we found that adult female mice (F0) who had experienced MS showed reduced locomotor activity and impairment of long‐term spatial and recognition memory. Analysis of various hippocampal regions showed that MS reduced the number of mature neurons in CA3 of females, which is perhaps a crucial hippocampal region for learning and memory; however, neurogenesis remained unchanged. In the second part, we measured maternal care in female mice with a history of early‐life stress (F0) as well as the behavior of their adult offspring (F1). Our results indicated that MS reduced the level of maternal care in adult females (F0) toward their own progeny and caused sex‐specific changes in the social behavior of adult offspring (F1). In contrast to MS, HD had no influence on female behavior or hippocampal plasticity. Overall, our results suggest that prolonged MS early in life affects the adult behavior of F0 female mice and hippocampal neuronal plasticity, whereas the mothers' previous experience has effects on the behavior of their F1 offspring through disturbances of mother‐infant interactions.

show abstract

The impact of early-life stress on the expression of HPA-associated genes in the adult murine brain

Cited by 15 publications

References 75 publications

Maternal Separation Early in Life Alters the Expression of Genes Npas4 and Nr1d1 in Adult Female Mice: Correlation with Social Behavior

Maternal Separation Early in Life Alters the Expression of Genes Npas4 and Nr1d1 in Adult Female Mice: Correlation with Social Behavior

Implications from early life stress on the development of mouse ovarian follicles: Focus on oxidative stress

Stress early in life leads to cognitive impairments, reduced numbers of CA3 neurons and altered maternal behavior in adult female mice

Contact Info

Product

Resources

About