The impact of Drew Noden's work on our understanding of craniofacial musculoskeletal integration

Nödl, Marie-Therese; Tsai, Stephanie; Galloway, Jenna L.

doi:10.1002/dvdy.471

Cited by 2 publications

(2 citation statements)

References 125 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differentiating between these possibilities will likely require a deeper understanding of gene regulatory networks (GRNs) regulating tendon progenitor specification, as Scx remains the earliest reported tendon marker 24,25 . Tendons in the craniofacial region like the MST are derived from cranial neural crest cells (CNCCs), while those in limbs are derived from the lateral plate mesoderm 26–28 . Regional differences in the GRNs underlying tendon development and regeneration are therefore likely to exist and have yet to be fully explored.…”

Section: Discussionmentioning

confidence: 99%

Endogenous Tenocyte Activation Underlies the Regenerative Capacity of Adult Zebrafish Tendon

Tsai

Villaseñor

Shah

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Tendons are essential, frequently injured connective tissues that transmit forces from muscle to bone. Their unique highly ordered, matrix-rich structure is critical for proper function. While adult mammalian tendons heal after acute injuries, endogenous tendon cells, or tenocytes, fail to respond appropriately, resulting in the formation of disorganized fibrovascular scar tissue with impaired function and increased propensity for re-injury. Here, we show that unlike mammals, adult zebrafish tenocytes activate upon injury and fully regenerate the tendon. Using a full tear injury model in the adult zebrafish craniofacial tendon, we defined the hallmark stages and cellular basis of tendon regeneration through multiphoton imaging, lineage tracing, and transmission electron microscopy approaches. Remarkably, we observe that the zebrafish tendon can regenerate and restore normal collagen matrix ultrastructure by 6 months post-injury (mpi). We show that tendon regeneration progresses in three main phases: inflammation within 24 hours post-injury (hpi), cellular proliferation and formation of a cellular bridge between the severed tendon ends at 3-5 days post-injury (dpi), and re-differentiation and matrix remodeling beginning from 5 dpi to 6 mpi. Importantly, we demonstrate that pre-existing tenocytes are the main cellular source of regeneration. Collectively, our work debuts the zebrafish tendon as one of the only reported adult tendon regenerative models and positions it as an invaluable comparative system to identify regenerative mechanisms that may inspire new tendon injury treatments in the clinic.

show abstract

Section: Discussionmentioning

confidence: 99%

Endogenous Tenocyte Activation Underlies the Regenerative Capacity of Adult Zebrafish Tendon

Tsai

Villaseñor

Shah

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Differentiating between these possibilities will likely require a deeper understanding of gene regulatory networks (GRNs) regulating tendon progenitor specification, as Scx remains the earliest reported tendon marker 26,27 . Tendons in the craniofacial region like the MST are derived from cranial neural crest cells (CNCCs), while those in limbs are derived from the lateral plate mesoderm 25,28,29 . Regional differences in the GRNs underlying tendon development and regeneration are therefore likely to exist and have yet to be fully explored.…”

Section: Discussionmentioning

confidence: 99%

Endogenous tenocyte activation underlies the regenerative capacity of the adult zebrafish tendon

Tsai,

Villaseñor,

Shah

et al. 2023

npj Regen Med

Self Cite

View full text Add to dashboard Cite

Tendons are essential, frequently injured connective tissues that transmit forces from muscle to bone. Their unique highly ordered, matrix-rich structure is critical for proper function. While adult mammalian tendons heal after acute injuries, endogenous tendon cells, or tenocytes, fail to respond appropriately, resulting in the formation of disorganized fibrovascular scar tissue with impaired function and increased propensity for re-injury. Here, we show that, unlike mammals, adult zebrafish tenocytes activate upon injury and fully regenerate the tendon. Using a full tear injury model in the adult zebrafish craniofacial tendon, we defined the hallmark stages and cellular basis of tendon regeneration through multiphoton imaging, lineage tracing, and transmission electron microscopy approaches. Remarkably, we observe that zebrafish tendons regenerate and restore normal collagen matrix ultrastructure by 6 months post-injury (mpi). Tendon regeneration progresses in three main phases: inflammation within 24 h post-injury (hpi), cellular proliferation and formation of a cellular bridge between the severed tendon ends at 3–5 days post-injury (dpi), and re-differentiation and matrix remodeling beginning from 5 dpi to 6 mpi. Importantly, we demonstrate that pre-existing tenocytes are the main cellular source of regeneration, proliferating and migrating upon injury to ultimately bridge the tendon ends. Finally, we show that TGF-β signaling is required for tenocyte recruitment and bridge formation. Collectively, our work debuts and aptly positions the adult zebrafish tendon as an invaluable comparative system to elucidate regenerative mechanisms that may inspire new therapeutic strategies.

show abstract

The impact of Drew Noden's work on our understanding of craniofacial musculoskeletal integration

Cited by 2 publications

References 125 publications

Endogenous Tenocyte Activation Underlies the Regenerative Capacity of Adult Zebrafish Tendon

Endogenous Tenocyte Activation Underlies the Regenerative Capacity of Adult Zebrafish Tendon

Endogenous tenocyte activation underlies the regenerative capacity of the adult zebrafish tendon

Contact Info

Product

Resources

About