The impact of diets with different magnesium contents on magnesium and calcium in serum and tissues of the rat

Zimmermann, Petra; Weiß, Ursula; Classen, H. G.; Wendt, Brigitte; Epple, August; Zöllner, Helmward; Temmel, Werner; Weger, Meltem; Porta, S.

doi:10.1016/s0024-3205(00)00688-3

Cited by 21 publications

(18 citation statements)

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“…Considering that the R163C RyR1 mutation in skeletal muscle resulted in a significantly increased cytosolic Ca 2ϩ (about 2-fold of the normal resting concentration, this study and that by Yang et al (30), an activation of several calcium-dependent pathways was expected leading to the activation of a slow-twitch fiber program to increase the resistance to fatigue, the oxidative capacity, and the calcium buffering capacity of mitochondria. The values for total calcium contents of WT mitochondria evaluated in this study were within the range of those previously reported for rodent skeletal muscle using ICP-MS or other techniques (82)(83)(84)(85)(86). The significantly higher concentrations of mitochondrial calcium content in R163C reported here may stem from the chronically elevated (2-4-fold) resting intracellular Ca 2ϩ concentrations in intact MHS skeletal muscle from various species (76 -78).…”

Section: Discussionsupporting

confidence: 84%

“…Given that exposure of mitochondria to a sustained higher calcium concentration may lead to higher calcium uptake and net calcium accumulation, dissipation of the electrochemical gradient, decreased ATP production, and/or organelle swelling or bursting (79 -81), the calcium concentrations in mitochondrial and PM fractions were evaluated in WT and R163C skeletal muscle by ICP-MS. The values for total calcium contents of WT were within those reported for rodent skeletal muscle using ICP-MS or other techniques (690 -1500 nmol/g muscle wet weight) (82)(83)(84)(85)(86). Skeletal muscle mitochondria from R163C mice had 5.7 times more Ca 2ϩ than WT, and the Ca 2ϩ concentration in the cytosolic fraction was 1.8-fold higher than WT (Table 4).…”

Section: Higher Mitochondrial Calcium In Ryr1 R163c Skeletal Muscle Isupporting

confidence: 73%

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Basal Bioenergetic Abnormalities in Skeletal Muscle from Ryanodine Receptor Malignant Hyperthermia-susceptible R163C Knock-in Mice

Giulivi

Ross-Inta

Omanska-Klusek

et al. 2011

Journal of Biological Chemistry

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Malignant hyperthermia (MH) and central core disease in humans have been associated with mutations in the skeletal ryanodine receptor (RyR1). Heterozygous mice expressing the human MH/central core disease RyR1 R163C mutation exhibit MH when exposed to halothane or heat stress. Considering that many MH symptoms resemble those that could ensue from a mitochondrial dysfunction (e.g. metabolic acidosis and hyperthermia) and that MH-susceptible mice or humans have a higher than normal cytoplasmic Ca 2؉ concentration at rest, we evaluated the role of mitochondria in skeletal muscle from R163C compared with wild type mice under basal (untriggered) conditions. R163C skeletal muscle exhibited a significant increase in matrix Ca 2؉ , increased reactive oxygen species production, lower expression of mitochondrial proteins, and higher mtDNA copy number. These changes, in conjunction with lower myoglobin and glycogen contents, Myh4 and GAPDH transcript levels, GAPDH activity, and lower glucose utilization suggested a switch to a compromised bioenergetic state characterized by both low oxidative phosphorylation and glycolysis. The shift in bioenergetic state was accompanied by a dysregulation of Ca 2؉ -responsive signaling pathways regulated by calcineurin and ERK1/2. Chronically elevated resting Ca 2؉ in R163C skeletal muscle elicited the maintenance of a fast-twitch fiber program and the development of insulin resistance-like phenotype as part of a metabolic adaptation to the R163C RyR1 mutation.

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Section: Discussionsupporting

confidence: 84%

Section: Higher Mitochondrial Calcium In Ryr1 R163c Skeletal Muscle Isupporting

confidence: 73%

Basal Bioenergetic Abnormalities in Skeletal Muscle from Ryanodine Receptor Malignant Hyperthermia-susceptible R163C Knock-in Mice

Giulivi

Ross-Inta

Omanska-Klusek

et al. 2011

Journal of Biological Chemistry

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“…However, there was no significant treatment effect on plasma Ca and P concentration. The current study result agrees with that of Zimmermann et al (2000) who reported that serum Mg of the rodents was highly dependent on dietary Mg level. Hess and Britton (1997) reported that excess Mg (dietary Mg levels of 0.15, 0.36, 0.53, 0.76, and 0.91%) reduced plasma Ca, and increased plasma Mg.…”

Section: Discussionsupporting

confidence: 93%

Effects of Magnesium Sources and Levels on Some Tissue Magnesium Concentration and Bone Mechanical Properties in Broiler

Çufadar¹,

Göçmen²,

Kanbur³

2017

SJAFS

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This experiment was conducted to determine the effect of inorganic and organic sources and levels of magnesium (Mg) supplementation on plasma, tibia, meat and liver Mg concentration and mechanical properties of bones in broilers. A total of one day old 450 broiler chicks were used and assigned to six experiment groups each having five replicate, randomly. There were 15 chicks in each replicates. In the experiment magnesium sulphate (MgSO 4 ) was used as inorganic Mg source and magnesium proteinate was used as organic Mg source. Experimental diets were supplemented provide 0 (control), 0.2 and 0.4 % Mg levels inorganic and organic Mg source of basal ration, and experiment period was six weeks. Main effect of Mg source and source x level interaction effect had not significant on plasma, liver, breast and thigh meat Mg concentration. While treatments did not significant effect on liver, breast and thigh meat Mg concentration, the main effect of Mg levels was significant effect on plasma Mg concentration. Plasma Mg concentration which were fed with ration added 0.2 and 0.4 % Mg level was higher than the control group. Tibia Ca concentration which were fed with ration added organic*0.2 and 0.4 % Mg levels were higher than the other groups. Tibia P concentration of organic Mg source fed with the group were higher than the inorganic source. The highest tibia Mg concentration were found to fed with organic*0.4 % Mg level of group. Tibia shear force and tibia stress which were fed with ration added 0.2 and 0.4 % Mg levels were higher than the control group. Supplementation of Mg in broiler diet was increased plasma Mg concentration and tibia shear force and also, tibia Ca and P concentration of organic Mg source fed with the group were higher than the inorganic Mg source.

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“…There is competitive inhibition of gradientdependent intestinal absorption, not only between magnesium and calcium 67,94 but also between magnesium and phosphorus provided calcium is adequate but magnesium absorption may increase at the expense of phosphorus when serum calcium is low 94 . Magnesium depletion is associated with increased serum ionised magnesium and calcium and decreased ionised phosphate 101,102 . Magnesium also interacts with vitamin D, such that magnesium deficiency impairs the synthesis of 1,25(OH)2D even during dietary calcium deprivation 176 and can lead on to resistance to 1,25(OH)2D 177 .…”

Section: Other Multinutrient Interactionsmentioning

confidence: 99%

Cardiovascular Calcification and Bone: A Comparison of The Effects of Dietary and Serum Calcium, Phosphorous, Magnesium and Vitamin D

Nicoll¹

2015

ICFJ

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This comparison of the effects of calcium, phosphorus, magnesium and vitamin D on cardiovascular (CV) calcification and bone has shown that in general the micronutrients that promote bone health also protect the arteries. We have shown that adequate amounts of all three minerals should be ingested, paying particular attention to supplementing calcium to bind high phosphorus and to ensure the maximum benefit from supplementing vitamin D. It appears that the optimum intake for bone is >800 mg/d calcium, with postmenopausal women possibly requiring a total intake of >1100mg/d. Both CV and bone studies suggest achieving a serum 25(OH)D level of >75nmol/l. These relationships are valid for a Caucasian population, however, and may not hold in African Americans and Asians. The recent concerns that calcium supplementation may increase CV disease risk has largely proved groundless, with higher calcium intake improving dyslipidaemia, hypertension and mortality. With respect to higher serum phosphate, there is an association with CV calcification and CVD risk even within the normal range, suggesting that the reference ranges may need to be redefined for ‘at risk’ patients. CV calcification was reduced in CKD patients with magnesium intake in the range 384-669mg/d. When considering the complex interplay of the action of the minerals together with their regulators vitamin D, PTH and FGF23, it is clear that this is a very sophisticated system which attempts to maintain calcium homeostasis to the possible detriment of bone and arteries. This reinforces the need to ensure adequate calcium intake before supplementing vitamin D.

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The impact of diets with different magnesium contents on magnesium and calcium in serum and tissues of the rat

Cited by 21 publications

References 0 publications

Basal Bioenergetic Abnormalities in Skeletal Muscle from Ryanodine Receptor Malignant Hyperthermia-susceptible R163C Knock-in Mice

Basal Bioenergetic Abnormalities in Skeletal Muscle from Ryanodine Receptor Malignant Hyperthermia-susceptible R163C Knock-in Mice

Effects of Magnesium Sources and Levels on Some Tissue Magnesium Concentration and Bone Mechanical Properties in Broiler

Cardiovascular Calcification and Bone: A Comparison of The Effects of Dietary and Serum Calcium, Phosphorous, Magnesium and Vitamin D

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