The impact of deuteration on natural and synthetic lipids: A neutron diffraction study

Luchini, Alessandra; Delhom, Robin; Demé, Bruno; Laux, Valerie; Moulin, Martine; Haertlein, Michael; Pichler, Harald; Strohmeier, Gernot A.; Wacklin, Hanna; Fragneto, Giovanna

doi:10.1016/j.colsurfb.2018.02.009

Cited by 29 publications

(27 citation statements)

References 56 publications

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“…hydrogen (H) and deuterium (D), by their different neutron cross sections 8 41,42 . Therefore, the studies based on H-D contrast are numerous [43][44][45][46][47][48][49]…”

Section: Introductionmentioning

confidence: 99%

Lipid Phase Separation Induced by the Apolar Polyisoprenoid Squalane Demonstrates Its Role in Membrane Domain Formation in Archaeal Membranes

et al. 2020

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Archaea synthesize methyl-branched, ether phospholipids, which confer the archaeal membrane exceptional physicochemical properties. A novel membrane organization was proposed recently to explain the thermal and high pressure tolerance of the polyextremophilic archaeon Thermococcus barophilus. According to this theoretical model, apolar molecules could populate the midplane of the bilayer and could alter the physicochemical properties of the membrane, among which is the possibility to form membrane domains. We tested this hypothesis using neutron diffraction on a model archaeal membrane composed of two archaeal diether lipids with phosphocholine and phosphoethanolamine headgroups in the presence of the apolar polyisoprenoid squalane. We show that squalane is inserted in the midplane at a maximal concentration between 5 and 10 mol % and that squalane can modify the lateral organization of the membrane and induces the coexistence of separate phases. The lateral reorganization is temperature-and squalane concentration-dependent and could be due to the release of lipid chain frustration and the induction of a negative curvature in the lipids.

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“…hydrogen (H) and deuterium (D), by their different neutron cross sections 8 41,42 . Therefore, the studies based on H-D contrast are numerous [43][44][45][46][47][48][49]…”

Section: Introductionmentioning

confidence: 99%

Lipid Phase Separation Induced by the Apolar Polyisoprenoid Squalane Demonstrates Its Role in Membrane Domain Formation in Archaeal Membranes

et al. 2020

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“…Despite the benefits of molecular deuteration in scattering techniques, there are some considerations to bear in mind; namely if the deuteration of lipid components truly reflects the same system when non-deuterated. Deuteration is known to affect the phase transition temperature of phospholipids 21,22 and the high resolution structural composition of lipid systems 22,23 . For low resolution studies, however, hydrogenous and deuterated lipid systems are often considered to be interchangeable.…”

Section: Introductionmentioning

confidence: 99%

The Production of Matchout-Deuterated Cholesterol and the Study of Bilayer-Cholesterol Interactions

Waldie

Moulin

Porcar

et al. 2019

Sci Rep

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The deuteration of biomolecules provides advanced opportunities for neutron scattering studies. For low resolution studies using techniques such as small-angle neutron scattering and neutron reflection, the level of deuteration of a sample can be varied to match the scattering length density of a specific D2O/H2O solvent mixture. This can be of major value in structural studies where specific regions of a complex system can be highlighted, and others rendered invisible. This is especially useful in analyses of the structure and dynamics of membrane components. In mammalian membranes, the presence of cholesterol is crucial in modulating the properties of lipids and in their interaction with proteins. Here, a protocol is described for the production of partially deuterated cholesterol which has a neutron scattering length density that matches that of 100% D2O solvent (hereby named matchout cholesterol). The level of deuteration was determined by mass spectrometry and nuclear magnetic resonance. The cholesterol match-point was verified experimentally using small angle neutron scattering. The matchout cholesterol was used to investigate the incorporation of cholesterol in various phosphatidylcholine supported lipid bilayers by neutron reflectometry. The study included both saturated and unsaturated lipids, as well as lipids with varying chain lengths. It was found that cholesterol is distributed asymmetrically within the bilayer, positioned closer to the headgroups of the lipids than to the middle of the tail core, regardless of the phosphatidylcholine species.

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“…In contrast, the methylotrophic yeast Pichia pastoris (also known as Komagataella pastoris and K. phaffii) has been used to produce highly deuterium-enriched chitosan (Russell et al, 2015), lipids (De Ghellinck et al, 2014;Gerelli et al, 2014;Luchini et al, 2018) and cholesterol (Moulin et al, 2018), and uniform partially deuterium-labeled heterologous eukaryotic proteins (Bodenheimer et al, 2018;Dunne et al, 2017) for neutron scattering studies. Each of the following is likely to have contributed to the choice of P. pastoris for producing deuterium-labeled materials: the high overexpression efficiencies of secreted and membrane-incorporated proteins per unit culture volume (Macauley-Patrick et al, 2005), the low concentration of natively secreted proteins (Cregg et al, 2000;Love et al, 2016) and the ability to use relatively inexpensive D 4 -labeled methanol as a carbon source.…”

Section: Labelingmentioning

confidence: 99%

Neutron scattering in the biological sciences: progress and prospects

Ashkar

Bilheux

Bordallo³

et al. 2018

Acta Crystallogr D Struct Biol

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The scattering of neutrons can be used to provide information on the structure and dynamics of biological systems on multiple length and time scales. Pursuant to a National Science Foundation‐funded workshop in February 2018, recent developments in this field are reviewed here, as well as future prospects that can be expected given recent advances in sources, instrumentation and computational power and methods. Crystallography, solution scattering, dynamics, membranes, labeling and imaging are examined. For the extraction of maximum information, the incorporation of judicious specific deuterium labeling, the integration of several types of experiment, and interpretation using high‐performance computer simulation models are often found to be particularly powerful.

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The impact of deuteration on natural and synthetic lipids: A neutron diffraction study

Cited by 29 publications

References 56 publications

Lipid Phase Separation Induced by the Apolar Polyisoprenoid Squalane Demonstrates Its Role in Membrane Domain Formation in Archaeal Membranes

Lipid Phase Separation Induced by the Apolar Polyisoprenoid Squalane Demonstrates Its Role in Membrane Domain Formation in Archaeal Membranes

The Production of Matchout-Deuterated Cholesterol and the Study of Bilayer-Cholesterol Interactions

Neutron scattering in the biological sciences: progress and prospects

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