The impact of cytokine gene polymorphisms on the outcome of HLA matched sibling hematopoietic stem cell transplantation

Kamel, Azza M.; Gameel, Abdallah; Ebid, Gamal; Radwan, Eman R.; Saleh, Mahasen; Abdelfattah, Raafat

doi:10.1016/j.cyto.2018.05.003

Cited by 8 publications

(3 citation statements)

References 58 publications

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“…These patients in group C had no mutation in the known genes responsible for FHL or other primary HLH. They possibly had other unknown gene mutations in T/NK cells and/or non-T/NK cells that predisposed them to the induction/maintenance of progressive HLH ( 37 – 39 ).…”

Section: Primary-ebv Hlhmentioning

confidence: 99%

Hematopoietic Stem Cell Transplantation for the Treatment of Epstein-Barr Virus-Associated T- or NK-Cell Lymphoproliferative Diseases and Associated Disorders

Sawada

Inoue

2018

Front. Pediatr.

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Chronic active Epstein-Barr virus infection (CAEBV) is a prototype of EBV-associated T- and/or NK-cell (EBV+ T/NK-cell) lymphoproliferative disorders. Most subtypes of these are lethal. We established a unified treatment strategy composed of step 1 (immunochemotherapy: steroids, cyclosporine A, and etoposide), step 2 (multi-drug block chemotherapy), and step 3 (allogeneic hematopoietic stem cell transplantation; HSCT) for CAEBV and its related diseases. Allogeneic HSCT is the only cure for CAEBV with few exceptions. Primary-EBV infection-associated hemophagocytic lymphohistiocytosis (primary-EBV HLH) is also an EBV+ T/NK-cell lymphoproliferation. The nature of EBV+ T/NK cells in CAEBV and those in primary-EBV HLH differ. In primary-EBV HLH, most patients need step 1 only and some require step 2 for the successful induction of apoptosis in EBV-infected T cells; however, some exceptional patients require HSCT. We herein present our single institutional experience of CAEBV and primary-EBV HLH, together with that of post-transplant EBV+ T/NK-cell lymphoproliferative disease. We also discuss some practical points on HCST with a review of the literature.

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Section: Primary-ebv Hlhmentioning

confidence: 99%

Hematopoietic Stem Cell Transplantation for the Treatment of Epstein-Barr Virus-Associated T- or NK-Cell Lymphoproliferative Diseases and Associated Disorders

Sawada

Inoue

2018

Front. Pediatr.

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“…37 The SNPs of cytokine genes mainly influence the production of proteins, which determines the microenvironment of the graft. 38,39 Pretransplant genetic testing of cytokine may provide a clinically useful means for risk stratification in solid organ transplant patients. In the present study, we explored the profiles of TNF-α (−308 A/G), TGF-β1 (+869 T/C), IL-10 (−592 C/A, −819 T/C and −1082 G/A), and IFN-γ (+874 T/A) SNPs in the LTRs and the control group.…”

Section: Discussionmentioning

confidence: 99%

High-resolution melting PCR analysis for genotyping the gene polymorphism of TNF-α, TGF-β1, IL-10, and IFN-γ in lung transplant recipients

Mu¹,

Zou²,

Ji³

et al. 2022

Adv Clin Exp Med

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Background. High-resolution melting (HRM) analysis is a genotyping method which has the advantages of simple, rapid, low-cost and closed-tube operation.Objectives. This study evaluated HRM analysis as an option for detecting the single nucleotide polymorphism (SNP) of cytokine, and profiled the distribution of cytokine gene polymorphism in the lung transplant recipients (LTRs). Materials and methods.High-resolution melting-polymerase chain reaction (HRM-PCR) assays for genotyping tumor necrosis factor alpha (TNF-α) (-308 A/G), tumor growth factor beta 1 (TGF-β1) (+869 T/C), interleukin 10 (IL-10) (-592 C/A, -819 T/C, -1082 G/A), and interferon gamma (IFN-γ) (+874 T/A) SNPs were developed on the LightCycler® 480. The SNPs of the aforementioned cytokine genes in 322 LTRs and 266 normal controls were detected using HRM-PCR approach. To confirm the accuracy of the HRM-PCR assay, we randomly selected 100 samples from the LTRs and detected the aforementioned SNPs with sequencespecific primer-polymerase chain reaction (SSP-PCR) method, using a commercial kit.Results. The data show that the HRM-PCR assay can distinguish all the cytokine SNPs, and the results of HRM-PCR analysis are in complete concordance to the genotyping results obtained using a commercial kit (κ = 1.0). Our data also show that the allele and genotype frequencies of the abovementioned cytokine are not significantly different between the LTRs and the control groups (p > 0.05). In addition, we found the genotypes of TGF-β1 +869 associated with high expression phenotype were prevalent in the LTRs. On the contrary, for TNF-α -308, IL-10 and IFN-γ, the genotypes associated with low expression phenotype were most common in the LTRs. Conclusions.In this study, we described a rapid, low-cost and high-throughput HRM-PCR technology for genotyping cytokine SNPs. Our data may be utilized for future studies examining the associations of cytokine gene polymorphisms with the prognosis of the LTRs.

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“…On the other hand, no support for IL1R or CTLA4 genes was identified by Hyvärinen et al (23). Two new studies (21,22) both report association of IFNG with cGvHD, but no evidence for this locus was found by Hyvärinen et al (23). The majority of studies have reported or focused on a small number of candidate loci or SNPs, and it is not clear whether negative findings were reported.…”

Section: Genetic Polymorphisms Associated With Cgvhdmentioning

confidence: 99%

Review of Genetic Variation as a Predictive Biomarker for Chronic Graft-Versus-Host-Disease After Allogeneic Stem Cell Transplantation

et al. 2020

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The impact of cytokine gene polymorphisms on the outcome of HLA matched sibling hematopoietic stem cell transplantation

Cited by 8 publications

References 58 publications

Hematopoietic Stem Cell Transplantation for the Treatment of Epstein-Barr Virus-Associated T- or NK-Cell Lymphoproliferative Diseases and Associated Disorders

Hematopoietic Stem Cell Transplantation for the Treatment of Epstein-Barr Virus-Associated T- or NK-Cell Lymphoproliferative Diseases and Associated Disorders

High-resolution melting PCR analysis for genotyping the gene polymorphism of TNF-α, TGF-β1, IL-10, and IFN-γ in lung transplant recipients

Review of Genetic Variation as a Predictive Biomarker for Chronic Graft-Versus-Host-Disease After Allogeneic Stem Cell Transplantation

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