The Impact of Copper Ions on Oxidative Stress in Garden Cress Lepidium sativum

Abstract

Normal oxygen metabolism is an endogenous source of reactive oxygen species (ROS). The source of ROS are also many environmental factors including heavy metals. In certain concentration range, the presence of ROS is necessary to maintain proper cell function. Thus, cells have many mechanisms, which role is focused on maintaining a constant concentration of ROS. Imbalance between the formation of ROS and action of a protective antioxidant system leads to oxidative stress. This may results with a damage to the structure of proteins, lipids and nucleic acids, which in turn can lead to disturbances in the functioning of the cell and even to the death. The aim of the study was to evaluate the effect of copper ions on the metabolic activity of garden cress Lepidium sativum L. The action of copper ions with different concentrations was treated seeds. After four, six and eight days after planting in the leaves of garden cress were determined the specific activity of guaiacol peroxidase (GPOX), lipid peroxidation and protein content. Additionally intake of copper ions was determined using adsorption spectrometry technique. The results revealed that the applied doses of copper ions affected the activity of guaiacol peroxidase. The highest enzyme activity was found in plant material, which was treated with dose of copper ions 1000 mg/dm 3 regardless of day. In the same samples the lowest level of lipid peroxidation was found. The highest concentrations of total proteins was found in samples treated with the highest dose of copper ions. The copper content in the tested plant material is correlated with the applied dose of copper ions. Our results indicate reliable correlations between copper content and values of oxidative stress biomarkers in plant tissues.

Functional Characterization of MaZIP4, a Gene Regulating Copper Stress Tolerance in Mulberry (Morus atropurpurea R.)

“…MDA is a widely used marker of environmental stress in plants [37]. The content of MDA and the activity of antioxidant enzymes is expected to increase initially and then decrease due to excessive Cu [38][39][40], which is consistent with our findings. Zhou et al (2015) studied the changes of physiological and biochemical reactions of forest trees under different levels of Pb stress, and showed that the activities of superoxide dismutase (SOD), peroxidase (POD) and MDA contents in trees were significantly increased under Pb stress [41].…”

Effect of Dissolve Organic Matter Nn Cd Contaminated Cotton (Gossypium Hirsutum L.) Growth and Cd Uptake

“…For this reason, the antioxidant system consisting of enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in plants scavenges ROS to ensure normal plant growth (Shahid et al 2014). At the same time, the production of ROS also increases malondialdehyde (MDA) activity, which is positively correlated with exogenous Cd stress concentration within a certain range (Rombel-Bryzek et al 2017). In this study, leaves MDA and CAT activities increased with Cd concentration, and SOD and POD activities decreased with Cd concentration, indicating that cotton membrane system damage was increased and metabolically generated H 2 O 2 was mainly scavenged by CAT (Wu et al 2020).…”

Curcumin Is an Iconic Ligand for Detecting Environmental Pollutants

“…Copper is an important environmental pollutant [ 78 ] and according to WHO, the tolerable concentrations of copper in drinking water is 2 ppm [ 79 ]. Excess levels of copper ions lead to oxidative stress, resulting in childhood cirrhosis, prion disease, Menkes disease, Parkinson's disease, and Wilson disease [ 80 ]. Similarly, the sulfide (S 2- ) anions are generated due to synthesis of sulfur and sulfuric acid, dyes, and cosmetics.…”