The impact of CO gradients on C. ljungdahlii in a 125 m3 bubble column: Mass transfer, circulation time and lifeline analysis

Siebler, Flora; Lapin, A. D.; Hermann, Maria

doi:10.1016/j.ces.2019.06.018

Cited by 34 publications

(36 citation statements)

References 33 publications

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“…An integrated GSM model was used to investigate targets for gene knockouts that improve cellular performance in industrial scale [ 40 ]. There also exist other models of large-scale bubble column fermentation where the biology was modeled with a fundamental set of reactions [ 269 ] or with a biothermodynamics approach [ 7 ]. Considering the industrial importance of bubble column reactors for gas fermentation [ 39 , 275 , 284 ], the rise of these models supports further scale-up and industrialization of gas fermentation.…”

Section: Systems Biology and Genetic Engineeringmentioning

confidence: 99%

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Vees

Neuendorf

Pflügl

2020

Journal of Industrial Microbiology and Biotechnology

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The sustainable production of solvents from above ground carbon is highly desired. Several clostridia naturally produce solvents and use a variety of renewable and waste-derived substrates such as lignocellulosic biomass and gas mixtures containing H2/CO2 or CO. To enable economically viable production of solvents and biofuels such as ethanol and butanol, the high productivity of continuous bioprocesses is needed. While the first industrial-scale gas fermentation facility operates continuously, the acetone–butanol–ethanol (ABE) fermentation is traditionally operated in batch mode. This review highlights the benefits of continuous bioprocessing for solvent production and underlines the progress made towards its establishment. Based on metabolic capabilities of solvent producing clostridia, we discuss recent advances in systems-level understanding and genome engineering. On the process side, we focus on innovative fermentation methods and integrated product recovery to overcome the limitations of the classical one-stage chemostat and give an overview of the current industrial bioproduction of solvents.

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Section: Systems Biology and Genetic Engineeringmentioning

confidence: 99%

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Vees

Neuendorf

Pflügl

2020

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

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“…McClure, Kavanagh, Fletcher, and Barton () studied exposure to excess sugar concentrations in a bubble column, whereas Kuschel et al () studied the presence of different regimes in replication behavior of P. putida in an industrial reactor. Most recently, Siebler, Lapin, Hermann, and Takors () used the approach to study CO limitations in syngas fermentation with C. Ljungdahlii , indicating 97% of cells experience substrate limitations, and 84% are likely to undergo transcription changes, after stress exposures of more than 70 s. Y. Liu et al () focused on strain‐rate variations observed by plant cells and their influence on viability loss, in what constitutes an evolution of the “Energy dissipation circulation function (EDCF)” method (Jüsten, Paul, Nienow, & Thomas, ).…”

Section: Through the Organism's Eyes: The Interaction Between Hydrodymentioning

confidence: 99%

“…Three different methods for lifeline analysis were developed by Haringa et al (). In regime analysis, the lifeline gets discretized into a limited number of regimes, each with a certain characteristic, such as substrate starvation and excess, overflow/byproduct formation, and so forth (Haringa et al, ; Kuschel et al, ; Siebler et al, ). The frequency at which cells switches between regimes and the duration of exposure to each regime are quantified.…”

Section: Through the Organism's Eyes: The Interaction Between Hydrodymentioning

confidence: 99%

Coupled metabolic‐hydrodynamic modeling enabling rational scale‐up of industrial bioprocesses

Wang

Haringa

Tang

et al. 2019

Biotech & Bioengineering

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Metabolomics aims to address what and how regulatory mechanisms are coordinated to achieve flux optimality, different metabolic objectives as well as appropriate adaptations to dynamic nutrient availability. Recent decades have witnessed that the integration of metabolomics and fluxomics within the goal of synthetic biology has arrived at generating the desired bioproducts with improved bioconversion efficiency. Absolute metabolite quantification by isotope dilution mass spectrometry represents a functional readout of cellular biochemistry and contributes to the establishment of metabolic (structured) models required in systems metabolic engineering. In industrial practices, population heterogeneity arising from fluctuating nutrient availability frequently leads to performance losses, that is reduced commercial metrics (titer, rate, and yield). Hence, the development of more stable producers and more predictable bioprocesses can benefit from a quantitative understanding of spatial and temporal cell-to-cell heterogeneity within industrial bioprocesses. Quantitative metabolomics analysis and metabolic modeling applied in computational fluid dynamics (CFD)-assisted scale-down simulators that mimic industrial heterogeneity such as fluctuations in nutrients, dissolved gases, and other stresses can procure informative clues for coping with issues during bioprocessing scale-up. In previous studies, only limited insights into the hydrodynamic conditions inside the industrial-scale bioreactor have been obtained, which makes case-by-case scale-up far from straightforward. Tracking the flow paths of cells circulating in large-scale bioreactors is a highly valuable tool for evaluating cellular performance in production tanks. The "lifelines" or "trajectories" of cells in industrial-scale bioreactors can be captured using Euler-Lagrange CFD simulation. This novel methodology can be further coupled with metabolic (structured) models to provide not only a statistical analysis of cell lifelines triggered by the environmental fluctuations but also a global assessment of the metabolic response to heterogeneity inside an industrial bioreactor. For the future, the industrial design should be dependent on the computational framework, and this integration work will allow bioprocess scale-up to the industrial scale with an end in mind. K E Y W O R D SCFD, Euler-Langrange, metabolic model, metabolomics, population heterogeneity, scale down

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“…The same applies for gaseous substrates in syngas fermentation. The availability of gaseous substrates for microbial cultivation has been mentioned in various reports (Bredwell et al, 1999; Keryanti et al, 2019; Siebler et al, 2019; Takors et al, 2018; Wan et al, 2017). However, to the best of our knowledge, online measurement of the gas transfer rates in syngas fermentation has been possible only at the fermenter scale.…”

Section: Introductionmentioning

confidence: 99%

Online monitoring of gas transfer rates during CO and CO/H₂ gas fermentation in quasi‐continuously ventilated shake flasks

Mann

Hüser

Schick

et al. 2021

Biotech & Bioengineering

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Syngas fermentation is a potential player for future emission reduction. The first demonstration and commercial plants have been successfully established. However, due to its novelty, development of syngas fermentation processes is still in its infancy, and the need to systematically unravel and understand further phenomena, such as substrate toxicity as well as gas transfer and uptake rates, still persists. This study describes a new online monitoring device based on the respiration activity monitoring system for cultivation of syngas fermenting microorganisms with gaseous substrates. The new device is designed to online monitor the carbon dioxide transfer rate (CO2TR) and the gross gas transfer rate during cultivation. Online measured data are used for the calculation of the carbon monoxide transfer rate (COTR) and hydrogen transfer rate (H2TR). In cultivation on pure CO and CO + H2, CO was continuously limiting, whereas hydrogen, when present, was sufficiently available. The maximum COTR measured was approximately 5 mmol/L/h for pure CO cultivation, and approximately 6 mmol/L/h for cultivation with additional H2 in the gas supply. Additionally, calculation of the ratio of evolved carbon dioxide to consumed monoxide, similar to the respiratory quotient for aerobic fermentation, allows the prediction of whether acetate or ethanol is predominantly produced. Clostridium ljungdahlii, a model acetogen for syngas fermentation, was cultivated using only CO, and CO in combination with H2. Online monitoring of the mentioned parameters revealed a metabolic shift in fermentation with sole CO, depending on COTR. The device presented herein allows fast process development, because crucial parameters for scale‐up can be measured online in small‐scale gas fermentation.

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The impact of CO gradients on C. ljungdahlii in a 125 m3 bubble column: Mass transfer, circulation time and lifeline analysis

Cited by 34 publications

References 33 publications

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Coupled metabolic‐hydrodynamic modeling enabling rational scale‐up of industrial bioprocesses

Online monitoring of gas transfer rates during CO and CO/H₂ gas fermentation in quasi‐continuously ventilated shake flasks

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The impact of CO gradients on C. ljungdahlii in a 125 m3 bubble column: Mass transfer, circulation time and lifeline analysis

Cited by 34 publications

References 33 publications

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Coupled metabolic‐hydrodynamic modeling enabling rational scale‐up of industrial bioprocesses

Online monitoring of gas transfer rates during CO and CO/H2 gas fermentation in quasi‐continuously ventilated shake flasks

Contact Info

Product

Resources

About

The impact of CO gradients on C. ljungdahlii in a 125 m3 bubble column: Mass transfer, circulation time and lifeline analysis

Online monitoring of gas transfer rates during CO and CO/H₂ gas fermentation in quasi‐continuously ventilated shake flasks